Iziselo zobisi ezineasidi ziye zanda kakhulu kwiminyaka yakutshanje ngenxa yeenzuzo zabo zempilo kunye nencasa eyodwa. Nangona kunjalo, ezi ziselo zinokuba ngumngeni ukuzinzisa, njengoko i-asidi ebisi inokubangela ukuba iiprotheni zidibanise kwaye zenze i-aggregates, ekhokelela kwi-sedimentation kunye nokwahlukana. Enye indlela esebenzayo yokuzinzisa iziselo zobisi ezine-asidi kukusetyenziswa kwe-carboxymethyl cellulose (CMC), i-polymer e-soluble yamanzi enokuthi idibanise neeprotheni kunye nezinye izithako zokwenza ukumiswa okuzinzileyo. Kweli nqaku, siza kuxubusha indlela yokusebenza yokuzinzisa iziselo zobisi ezine-asidi yi-CMC.

Ubume kunye neePropati zeCMC

I-CMC iphuma kwi-cellulose, ipholima yendalo efumaneka kwiindonga zeeseli zezityalo. Yenziwe ngokuguqulwa kweekhemikhali i-cellulose kunye namaqela e-carboxymethyl, ephucula ukunyibilika kwamanzi kunye nezinye iimpawu. I-CMC yipolymer enamasebe amakhulu kunye nomqolo omde wekhonkco kunye namatyathanga amaninzi asecaleni amaqela e-carboxymethyl. Iqondo lokutshintshela (DS) le-CMC libhekiselele kwinani lamaqela e-carboxymethyl ngeyunithi ye-cellulose, kwaye imisela iqondo lokunyibilika kunye ne-viscosity ye-CMC.

Inkqubo yokuSebenza ye-CMC ekuzinziseni iziselo zobisi olune-Acidified

Ukongezwa kwe-CMC kwiziselo zobisi ezineasidi kunokuphucula uzinzo ngeendlela ezininzi:

1. I-Electrostatic Repulsion: Amaqela e-carboxymethyl kwi-CMC ahlawuliswa kakubi kwaye angakwazi ukusebenzisana neeprotheyini ezihlawulwe ngokufanelekileyo kunye nezinye izithako ebisini, ezenza amandla aphikisayo avimbela iiprotheni ukuba zidibanise kwaye zihlale. Oku kugxothwa kwe-electrostatic kuzinzisa ukunqunyanyiswa kunye nokuthintela intlenga.
2. Ukusebenzisana kwe-Hydrophilic: Ubume be-hydrophilic ye-CMC buvumela ukuba idibanise iimolekyuli zamanzi kunye nezinye izinto ze-hydrophilic ebisini, zenze umaleko okhuselayo ojikeleze iiprotheni kunye nokuthintela ukusebenzisana kwazo.
3. Isithintelo esiNcinci: Ubume besebe leCMCinokudala impembelelo yokuthintela i-steric, ithintele iiproteni ukuba zingadibanisi kwaye zenze ii-aggregates. Amatyathanga amade, aguquguqukayo e-CMC nawo ayakwazi ukujikeleza amasuntswana eprotheyini, enze umqobo othintela ukuba angadibani omnye nomnye.
4. I-Viscosity: Ukongezwa kwe-CMC kwiziselo zobisi ezine-asidi kunokunyusa i-viscosity yazo, enokuthi ithintele i-sedimentation ngokunciphisa isantya sokumisa amasuntswana. Ukwanda kwe-viscosity kunokudala ukunqunyanyiswa okuzinzileyo ngokuphucula intsebenziswano phakathi kwe-CMC kunye nezinye izithako kubisi.

Imiba echaphazela ukuzinziswa kweziselo zobisi olune-Acidified yi-CMC

Ukusebenza kwe-CMC ekuzinziseni iziselo zobisi ezineasidi kuxhomekeke kwizinto ezininzi, kuquka:

1. I-pH: Ukuzinza kweziselo zobisi ezineasidi kuphenjelelwa kakhulu yi-pH. Kwixabiso eliphantsi le-pH, iiprotheyini ezisebisini ziye zenziwa i-denatured kwaye zenze i-aggregates ngokulula, nto leyo eyenza uzinzo lube ngumngeni ngakumbi. I-CMC ingazinzisa iziselo zobisi ezineasidi kumaxabiso e-pH asezantsi njenge-3.5, kodwa ukusebenza kwawo kuyehla kumaxabiso aphantsi e-pH.
2. Ukugxila kwe-CMC: Ukuxinana kwe-CMC kubisi kuchaphazela iimpawu zayo zokuzinzisa. Ukugxininiswa okuphezulu kwe-CMC kunokukhokelela ekwandeni kwe-viscosity kunye nokuphuculwa kozinzo, kodwa ukugxila okuphezulu kakhulu kunokubangela ukuthungwa kunye nencasa engafunekiyo.
3. Ugxininiso lweProtheyini: Ukugxininiswa kunye nohlobo lweeprotheni ebisi kunokuchaphazela ukuzinza kwesiselo. I-CMC iyasebenza kakhulu ekuzinziseni iziselo ezinoxinzelelo lweprotheyini ephantsi, kodwa inokuzinzisa iziselo ezinoxinzelelo oluphezulu lweprotheyini ukuba amasuntswana eprotheyini mancinci kwaye asasazwe ngokulinganayo.
4. Iimeko Zokulungisa: Iimeko zokulungiswa ezisetyenziselwa ukuvelisa isiselo sobisi esineasidi zinokuchaphazela ukuzinza kwaso. Amandla aphezulu okucheba kunye nokushisa kunokubangela iprotheni denaturation kunye nokuhlanganiswa, okukhokelela ekungazinzini. Iimeko zokulungisa kufuneka ziphuculwe ukunciphisa iprotheni.