Kugadzirira kweHydrogel Microspheres kubva kuHydroxypropyl Methyl Cellulose

Kuedza uku kunotora reverse phase suspension polymerization nzira, uchishandisa hydroxypropyl methylcellulose (HPMC) seyakagadzirwa, sodium hydroxide solution sechikamu chemvura, cyclohexane sechikamu chemafuta, uye divinyl sulfone (DVS) semuchinjiko-unobatanidza Musanganiswa weTween- 20 uye Span-60 seanoparadzanisa, achikurudzira pakukurumidza kwe400-900r / min kugadzirira hydrogel microspheres.

Mazwi akakosha: hydroxypropyl methylcellulose; hydrogel; microspheres; dispersant

1.Overview

1.1 Tsanangudzo yehydrogel

Hydrogel (Hydrogel) imhando yepamusoro molecular polymer iyo ine yakawanda yemvura mune network chimiro uye isinganyungudika mumvura. Chikamu chemapoka e-hydrophobic uye hydrophilic residues inounzwa mumvura-inonyungudika polymer ine network yakachinjika chimiro, uye iyo hydrophilic Izvo zvakasara zvinosunga kumamorekuru emvura, zvichibatanidza mamorekuru emvura mukati metiweki, nepo masara ehydrophobic anozvimba nemvura kuti aite muchinjiko. -akabatanidzwa mapolima. Jellies uye contact lens muupenyu hwezuva nezuva zvose hydrogel zvigadzirwa. Maererano nehukuru uye chimiro che hydrogel, inogona kukamurwa kuva macroscopic gel uye microscopic gel (microsphere), uye yekare inogona kugoverwa kuva columnar, porous sponge, fibrous, membranous, spherical, etc. Ikozvino yakagadzirirwa microspheres uye nanoscale microspheres. kuve nekupfava kwakanaka, elasticity, kuchengetedza kwemvura uye biocompatibility, uye inoshandiswa mukutsvaga kwemishonga yakabatwa.

1.2 Kukosha kwekusarudza musoro wenyaya

Mumakore achangopfuura, kuitira kuti isangane nezvinodiwa zvekuchengetedzwa kwezvakatipoteredza, polymer hydrogel zvinhu zvishoma nezvishoma zvakakwezva kutarisisa kwakapararira nekuda kwezvakanaka hydrophilic zvimiro uye biocompatibility. Hydrogel microspheres yakagadzirirwa kubva kuhydroxypropyl methylcellulose seyakagadzirwa muyedzo iyi. Hydroxypropyl methylcellulose isiri-ionic cellulose ether, hupfu chena, isinganhuhwi uye isingaravike, uye ine hunhu husingadzokerike hwezvimwe zvekugadzira polymer zvinhu, saka ine yakakwirira kutsvagisa kukosha mumunda wepolymer.

1.3 Chimiro chebudiriro munyika nekune dzimwe nyika

Hydrogel imhando yemishonga yemishonga iyo yakakwezva kutarisisa kwakawanda munharaunda yezvekurapa yepasirese mumakore achangopfuura uye yakakurumidza kukura. Sezvo Wichterle naLim vakaburitsa basa ravo rekupayona paHEMA cross-linked hydrogels muna 1960, tsvakiridzo uye kuongororwa kwemahydrogel kwakaramba kuchidzika. Pakati pe-1970s, Tanaka akawana pH-sensitive hydrogels paaiyera chiyero chekuzvimba chechembere acrylamide gels, ichimaka nhanho nyowani mukudzidza kwemahydrogel. nyika yangu iri padanho rekuvandudzwa kwehydrogel. Nekuda kwegadziriro yakakura yegadziriro yemishonga yechinyakare yechiChinese uye zvinhu zvakaomarara, zvinonetsa kuburitsa chinhu chimwe chete chakachena kana akawanda mazvikamu achishanda pamwe chete, uye dosi yacho yakakura, saka kuvandudzwa kwemushonga weChinese hydrogel kunogona kunonoka.

1.4 Zvishandiso zvekuyedza nemisimboti

1.4.1 Hydroxypropyl methylcellulose

Hydroxypropyl methyl cellulose (HPMC), yakabva kumethyl cellulose, yakakosha yakasanganiswa ether, inova isiri-ionic mvura-inonyungudika mapolymers, uye haina hwema, isinganakidze uye isina chepfu.

Industrial HPMC iri muchimiro chehupfu chena kana chena yakasununguka faibha, uye mhinduro yayo ine aqueous ine basa repamusoro, kujeka kwepamusoro uye kushanda kwakagadzikana. Nekuti HPMC ine pfuma yekupisa gelation, chigadzirwa aqueous mhinduro inodziya kuti igadzire gel uye inonaya, uye yobva yanyungudika mushure mekutonhora, uye tembiricha yegelation yezvakasiyana zvechigadzirwa zvakasiyana. Izvo zvimiro zvezvakasiyana zvakatemwa zveHPMC zvakare zvakasiyana. Iyo solubility inoshanduka ne viscosity uye haina kukanganiswa ne pH kukosha. Iyo yakaderera viscosity, iyo yakakura solubility. Sezvo zviri muboka remethoxyl zvinodzikira, iyo gel point yeHPMC inowedzera, kunyungudika kwemvura kunoderera, uye basa repamusoro rinodzikira. Muindasitiri yebiomedical, inonyanya kushandiswa seyero-inodzora polymer zvinhu zvekupfekedza zvinhu, zvemufirimu zvinhu, uye gadziriro-yakaburitswa-kuburitswa. Inogona zvakare kushandiswa seyakagadzikana, inomisa mumiriri, inonamira piritsi, uye viscosity enhancer.

1.4.2 Musimboti

Uchishandisa reverse phase suspension polymerization method, uchishandisa Tween-20, Span-60 compound dispersant uye Tween-20 sevaparadziri vakasiyana, sarudza kukosha kweHLB (surfactant is an amphiphile with hydrophilic group uye lipophilic group Molecule, uwandu hwehukuru uye simba. kuenzana pakati peboka re hydrophilic neboka re lipophilic mu surfactant molecule inotsanangurwa seyakaenzana yehuwandu hwehuwandu hwehydrophilic-lipophilic yeCyclohexane inoshandiswa sechikamu chemafuta cheCyclohexane inogona kuparadzira zviri nani mhinduro ye monomer mukuedza kuenderera mberi, 1-5 nguva yemonomer aqueous solution Iine 99% divinyl sulfone semuchinjiro we-cross-linking agent, uye chiyero che-cross-linking agent chinodzorwa pane 10% ye. iyo yakaoma cellulose mass, kuitira kuti akawanda mutsara mamorekuru anosungirirwa kune rimwe nerimwe uye kuyambuka-akabatanidzwa mune network chimiro Chinhu chinosungirirana kana kufambisa kana ionic bond kuumbwa pakati pepolymer molecular cheni.

Kukurudzira kwakakosha pakuyedza uku, uye kumhanya kunowanzo kudzorwa pagiya rechitatu kana rechina. Nekuti saizi yekutenderera kumhanya inobata zvakananga saizi ye microspheres. Kana kukurumidza kutenderera kwakakura kupfuura 980r/min, pachava neyakakomba yemadziro inonamira chiitiko, iyo inozoderedza zvakanyanya goho rechigadzirwa; Muchinjikwa-muchinjika unowanzo gadzira gels dzakawanda, uye zvigadzirwa zve spherical hazvigone kuwanikwa.

2. Zvishandiso zvekuedza uye nzira

2.1 Kuedza Zviridzwa

Electronic balance, multifunctional electric stirrer, polarizing microscope, Malvern particle size analyzer.

Kugadzirira cellulose hydrogel microspheres, makemikari makuru anoshandiswa cyclohexane, Tween-20, Span-60, hydroxypropyl methylcellulose, divinyl sulfone, sodium hydroxide, mvura yakasvibiswa, iyo yose iyo Monomers uye additives inoshandiswa zvakananga pasina kurapwa.

2.2 Kugadzirira matanho e cellulose hydrogel microspheres

2.2.1 Kushandisa Tween 20 se dispersant

Kuparara kwehydroxypropylmethylcellulose. Nyatsoyera 2g yesodium hydroxide uye gadzira 2% sodium hydroxide solution ine 100ml volumetric flask. Tora 80ml yeyakagadzirirwa sodium hydroxide solution woipisa mubhavhu remvura kusvika pamakumi mashanu°C, uremu 0.2g ye cellulose uye woiwedzera kune alkaline solution, simbisai netsvimbo yegirazi, iise mumvura inotonhora yekugeza echando, uye shandisa iyo sechikamu chemvura mushure mokunge mhinduro yacho yajekeswa. Shandisa silinda yakapedza kudzidza kuyera 120ml ye cyclohexane (chikamu chemafuta) mubhokisi rine mitsipa mitatu, tora 5ml yeTween-20 muchikamu chemafuta nesirinji, uye simbisa pa 700r / min kweawa imwe. Tora hafu yechikamu chakagadzirirwa chemvura uye uwedzere kune furasiki ine mitsipa mitatu uye simbisa kwemaawa matatu. Iko kusanganiswa kwe divinyl sulfone ndeye 99%, yakaderedzwa kusvika 1% nemvura yakasvibiswa. Shandisa pipette kutora 0.5ml yeDVS mu50ml volumetric flask kugadzira 1% DVS, 1ml yeDVS yakaenzana ne0.01g. Shandisa pipette kutora 1ml mumoto wemitsipa mitatu. Kurudzira pakamuri tembiricha kwemaawa makumi maviri nemaviri.

2.2.2 Kushandisa span60 neTween-20 sevanoparadzira

Imwe hafu yechikamu chemvura ichangobva kugadzirirwa. Weresa 0.01gspan60 uye woiwedzera kune test tube, kupisa mumvura yekugezera ye65-degree kusvika yanyunguduka, wobva wadonhedza madonhwe mashoma ecyclohexane mumvura yekugezesa nerubber dropper, woipisa kusvika mhinduro yachinja mukaka chena. Iwedzere kune katatu-mitsipa flask, wobva wawedzera 120ml ye cyclohexane, simesa test tube ne cyclohexane kakawanda, kupisa kwe5min, kutonhora kusvika kune imwe nzvimbo, uye kuwedzera 0.5ml yeTween-20. Mushure mekukurudzira kwemaawa matatu, 1ml yeDVS yakasvibiswa yakawedzerwa. Kurudzira pakamuri tembiricha kwemaawa makumi maviri nemaviri.

2.2.3 Zvakabuda mukuedza

Sample yakamutswa yakanyikwa mutsvimbo yegirazi uye yakanyungudutswa mu50ml yeabsolute ethanol, uye saizi yechikamu yakayerwa pasi peMalvern particle sizer. Kushandisa Tween-20 se dispersant microemulsion yakakura, uye yakayerwa particle size ye 87.1% i455.2d.nm, uye saizi ye12.9% i5026d.nm. Iyo microemulsion yeTween-20 uye Span-60 yakasanganiswa dispersant yakafanana neyemukaka, ine 81.7% particle size ye5421d.nm uye 18.3% particle size ye180.1d.nm.

3. Hurukuro yemigumisiro yekuedza

Kune iyo emulsifier yekugadzirira inverse microemulsion, kazhinji zviri nani kushandisa musanganiswa we hydrophilic surfactant uye lipophilic surfactant. Izvi zvinodaro nekuti kunyungudika kweimwe surfactant musystem kwakadzikira. Mushure mekunge maviri aya asanganiswa, mapoka emumwe nemumwe ane hydrophilic mapoka uye lipophilic mapoka anoshandira pamwe chete kuti ave nesolubilizing mhedzisiro. HLB kukosha indekisi inowanzoshandiswa pakusarudza emulsifiers. Nekugadzirisa kukosha kweHLB, chiyero chezvikamu zviviri emulsifier inogona kugadziriswa, uye mamwe mayunifomu ma microspheres anogona kugadzirwa. Muchiedzo ichi, isina simba lipophilic Span-60 (HLB = 4.7) uye hydrophilic Tween-20 (HLB = 16.7) yakashandiswa seanoparadzira, uye Span-20 yakashandiswa yoga seanoparadzira. Kubva pamigumisiro yekuedza, zvinogona kuonekwa kuti musanganiswa Mhedzisiro iri nani pane imwe chete inoparadzira. Iyo microemulsion yekomponi dispersant ine yunifomu uye ine mukaka-kufanana kuenderana; iyo microemulsion inoshandisa dispersant imwechete ine yakawandisa viscosity uye machena particles. Iyo diki yepamusoro inoonekwa pasi peiyo komputa inoparadzira yeTween-20 uye Span-60. Chikonzero chinogoneka ndechekuti kupindirana kwemukati kweiyo kompani system yeSpan-60 uye Tween-20 yakakwirira, uye dispersant pachayo yakaputsika pasi pepamusoro-soro inokurudzira kuumba Iyo yakanaka particles ichakanganisa mhedzisiro yekuedza. Kuipa kweiyo dispersant Tween-20 ndeyekuti ine nhamba yakakura ye polyoxyethylene cheni (n = 20 kana zvakadaro), izvo zvinoita kuti steric chipingamupinyi pakati pe surfactant molecules ive yakakura uye yakaoma kuve dense pane iyo interface. Tichitarisa kubva mukusanganiswa kwematayagiramu esaizi, zvimedu zvichena zviri mukati zvinogona kunge zvisina cellulose. Nokudaro, migumisiro yekuedza iyi inoratidza kuti kushandiswa kwekushandisa komputa dispersant kuri nani, uye kuedza kunogona kuwedzera kuderedza huwandu hweTween-20 kuita kuti microspheres yakagadzirirwa yunifomu.

Pamusoro pezvo, zvimwe zvikanganiso mukuyedza mashandiro ekuita zvinofanirwa kudzikiswa, sekugadzirira kwesodium hydroxide mukugadzirisa kweHPMC, dilution yeDVS, nezvimwewo, inofanirwa kuve yakajairwa sezvinobvira kuderedza zvikanganiso zvekuedza. Chinhu chinonyanya kukosha chiyero chekuparadzira, kukurumidza uye kusimba kwekukurudzira, uye chiyero chemuchinjiro wekubatanidza. Chete kana ichinyatsodzorwa zvakanaka ndipo panogona kugadzirirwa hydrogel microspheres ine kupararira kwakanaka uye yunifomu saizi yemhando.