Gutegura Microsperes ya Hydrogel kuva Hydroxypropyl Methyl Cellulose

Ubu bushakashatsi bwifashishije uburyo bwo guhagarika icyiciro cya polymerisiyoneri, hifashishijwe hydroxypropyl methylcellulose (HPMC) nkibikoresho fatizo, igisubizo cya sodium hydroxide nkicyiciro cyamazi, cyclohexane nkicyiciro cyamavuta, na divinyl sulfone (DVS) nkumuvanga uhuza Tween- 20 na Span-60 nkikwirakwiza, bikurura umuvuduko wa 400-900r / min kugirango utegure microsperes ya hydrogel.

Amagambo y'ingenzi: hydroxypropyl methylcellulose; hydrogel; mikorobe; gutatanya

1.Incamake

1.1 Ibisobanuro bya hydrogel

Hydrogel (Hydrogel) ni ubwoko bwa polymer ndende cyane irimo amazi menshi mumiterere y'urusobe kandi ntishobora gushonga mumazi. Igice cyamatsinda ya hydrophobique hamwe n ibisigazwa bya hydrophilique byinjizwa mumazi ya elegitoronike yamazi hamwe numuyoboro uhuza imiyoboro, hamwe na hydrophilique Ibisigisigi bihuza na molekile zamazi, bigahuza molekile zamazi imbere murusobe, mugihe ibisigazwa bya hydrophobi byabyimbye n'amazi kugirango bibe umusaraba. -bihuza polimeri. Jellies hamwe na lens ya contact mubuzima bwa buri munsi nibicuruzwa bya hydrogel. Ukurikije ubunini n'imiterere ya hydrogel, irashobora kugabanywa muri macroscopique gel na microscopique gel (microsphere), naho iyambere irashobora kugabanywamo inkingi, sponge pore, fibrous, membrane, spherical, nibindi. ufite ubwitonzi bwiza, ubworoherane, ubushobozi bwo kubika amazi hamwe na biocompatibilité, kandi bikoreshwa mubushakashatsi bwibiyobyabwenge byafashwe.

1.2 Akamaro ko guhitamo ingingo

Mu myaka yashize, kugira ngo huzuzwe ibisabwa mu kurengera ibidukikije, ibikoresho bya polymer hydrogel byagiye bikurura abantu benshi kubera imiterere myiza ya hydrophilique na biocompatibilité. Microspheres ya hydrogel yateguwe kuva hydroxypropyl methylcellulose nkibikoresho fatizo muri ubu bushakashatsi. Hydroxypropyl methylcellulose ni selile ya ionic selulose ether, ifu yera, impumuro nziza kandi itaryoshye, kandi ifite ibimenyetso bidasubirwaho nibindi bikoresho bya polymer synthique, kubwibyo bifite agaciro gakomeye mubushakashatsi mumashanyarazi.

1.3 Imiterere yiterambere murugo no hanze

Hydrogel nuburyo bwa farumasi yimiti yakwegereye cyane mumiryango mpuzamahanga yubuvuzi mumyaka yashize kandi yateye imbere byihuse. Kuva Wichterle na Lim basohora umurimo wabo w'ubupayiniya kuri HEMA ihuza hydrogels mu 1960, ubushakashatsi n'ubushakashatsi bwa hydrogel byakomeje kwiyongera. Mu myaka ya za 70 rwagati, Tanaka yavumbuye hydrogène yunvikana na pH igihe yapimaga igipimo cyo kubyimba geli ya acrylamide ishaje, ibyo bikaba ari intambwe nshya mu bushakashatsi bwa hydrogel. igihugu cyanjye kiri mubyiciro byiterambere rya hydrogel. Bitewe nuburyo bunini bwo gutegura imiti gakondo yubushinwa hamwe nibigize ibintu bigoye, biragoye gukuramo ibicuruzwa bimwe byera mugihe ibice byinshi bikorana, kandi dosiye nini, bityo iterambere ryubuvuzi bwa hydrogel hydrogel rishobora gutinda.

1.4 Ibikoresho by'igerageza n'amahame

1.4.1 Hydroxypropyl methylcellulose

Hydroxypropyl methyl selulose (HPMC), ikomoka kuri methyl selulose, ni ether ikomeye ivanze, ikaba ari iy'amazi adafite ionic polymers polymers, kandi nta mpumuro nziza, itaryoshye kandi idafite uburozi.

Inganda HPMC iri muburyo bwa poro yera cyangwa fibre yera yera, kandi igisubizo cyamazi gifite ibikorwa byubuso, gukorera mu mucyo no gukora neza. Kuberako HPMC ifite umutungo wa gelasi yubushyuhe, ibicuruzwa byamazi yumuriro birashyuha kugirango bibe gel hamwe nigishitsi, hanyuma bigashonga nyuma yo gukonja, nubushyuhe bwa gelation bwibintu bitandukanye biranga ibicuruzwa biratandukanye. Ibiranga ibintu bitandukanye bya HPMC nabyo biratandukanye. Gukemura birahinduka hamwe nubwiza kandi ntibiterwa nagaciro ka pH. Hasi ya viscosity, niko gukomera. Mugihe ibiri mumatsinda ya mikorerexyl bigabanuka, gel gel ya HPMC iriyongera, amazi yo kugabanuka aragabanuka, kandi ibikorwa byubutaka bigabanuka. Mu nganda zikomoka ku binyabuzima, zikoreshwa cyane cyane nkibikoresho bigenzura igipimo cya polymer kubikoresho byo gutwikira, ibikoresho bya firime, hamwe nimyiteguro irekura. Irashobora kandi gukoreshwa nka stabilisateur, guhagarika agent, gufata ibinini, hamwe no kongera ububobere.

1.4.2 Ihame

Ukoresheje uburyo bwa reaction yo guhagarika polymerisiyoneri, ukoresheje Tween-20, Span-60 ikomatanya ikwirakwiza hamwe na Tween-20 nkibitandukanya bitandukanye, menya agaciro ka HLB (surfactant ni amphiphile hamwe nitsinda rya hydrophilique hamwe na lipophilique group Molecule, ingano yubunini n'imbaraga uburinganire hagati yitsinda rya hydrophilique nitsinda rya lipofilique muri molekile ya surfactant isobanurwa nkurugero rwagereranijwe rwa hydrophilique-lipophilic balanse ya surfactant. Cyclohexane ikoreshwa nkicyiciro cyamavuta kandi Cyclohexane irashobora gukwirakwiza igisubizo cya monomer mubigeragezo ubudahwema Igipimo cyikubye inshuro 1-5 cyumuti wamazi wa monomer Hamwe na concentration ya 99% divinyl sulfone nkumukozi uhuza, kandi umubare wibikorwa bihuza bigenzurwa hafi 10% ya misa yumye ya selile, kuburyo molekile nyinshi zumurongo zifatanijwe hamwe kandi zigahuza imiterere y'urusobekerane.

Gukurura ni ngombwa cyane kuri ubu bushakashatsi, kandi umuvuduko muri rusange ugenzurwa nibikoresho bya gatatu cyangwa icya kane. Kuberako ingano yumuvuduko wo kuzenguruka igira ingaruka itaziguye ku bunini bwa microsperes. Iyo umuvuduko wo kuzenguruka urenze 980r / min, hazabaho ibintu bikomeye byo gufunga urukuta, bizagabanya cyane umusaruro wibicuruzwa; Umukozi uhuza ibice bikunda kubyara gele nyinshi, kandi ibicuruzwa ntibishobora kuboneka.

2. Ibikoresho byubushakashatsi nuburyo

2.1 Ibikoresho by'igerageza

Impuzandengo ya elegitoronike, imashini ikora amashanyarazi menshi, microscope ya polarize, isesengura rya ingano ya Malvern.

Gutegura microsperes ya hydrogel hydrogel, imiti nyamukuru ikoreshwa ni cyclohexane, Hagati ya 20, Span-60, hydroxypropyl methylcellulose, divinyl sulfone, hydroxide ya sodium, amazi yatoboye, yose Monomers ninyongeramusaruro zikoreshwa muburyo butavuwe.

2.2 Intambwe zo gutegura selile ya hydrogel microsperes

2.2.1 Gukoresha Hagati ya 20 nkuwatatanye

Iseswa rya hydroxypropylmethylcellulose. Gupima neza 2g ya hydroxide ya sodium hanyuma utegure 2% sodium hydroxide yumuti hamwe na flask ya volmetric 100ml. Fata 80ml yumuti wa sodium hydroxide wateguwe hanyuma ubishyuhe mubwogero bwamazi kugeza kuri 50°C. Koresha silinderi yarangije gupima 120ml ya cyclohexane (icyiciro cyamavuta) mumashanyarazi yamajosi atatu, shushanya 5ml ya Tween-20 mugice cyamavuta hamwe na syringe, hanyuma ukangure kuri 700r / min kumasaha imwe. Fata kimwe cya kabiri cyicyiciro cyamazi cyateguwe hanyuma wongere kuri flask yamajosi atatu hanyuma ukangure amasaha atatu. Ubunini bwa divinyl sulfone ni 99%, bivangwa na 1% n'amazi yatoboye. Koresha umuyoboro ufata 0.5ml ya DVS mumashanyarazi ya 50ml kugirango utegure 1% DVS, 1ml ya DVS ihwanye na 0.01g. Koresha pipette kugirango ufate 1ml mumashanyarazi atatu. Kangura ubushyuhe bwicyumba kumasaha 22.

2.2.2 Ukoresheje span60 na Tween-20 nkabatatanye

Igice cya kabiri cyicyiciro cyamazi cyateguwe. Gupima 0.01gspan60 hanyuma uyongereze mumiyoboro yipimisha, shyushya mubwogero bwa dogere 65 kugeza ushonge, hanyuma utere ibitonyanga bike bya cyclohexane mubwogero bwamazi hamwe nigitonyanga, hanyuma ubishyuhe kugeza igisubizo gihindutse amata yera. Ongera kuri flask yamajosi atatu, hanyuma ongeramo 120ml ya cyclohexane, kwoza umuyoboro wikizamini hamwe na cyclohexane inshuro nyinshi, shyushya 5min, ukonje kugeza ubushyuhe bwicyumba, hanyuma wongereho 0.5ml ya Tween-20. Nyuma yo gukurura amasaha atatu, 1ml ya DVS yongewemo. Kangura ubushyuhe bwicyumba kumasaha 22.

2.2.3 Ibisubizo byubushakashatsi

Icyitegererezo cyakuweho cyinjijwe mu nkoni yikirahure hanyuma gishonga muri 50ml ya Ethanol yuzuye, kandi ingano yapimwe yapimwe munsi yubunini bwa Malvern. Gukoresha Tween-20 nka microemuliyoni ikwirakwiza ni ndende, kandi ingano yapimwe ingana na 87.1% ni 455.2d.nm, naho ingano ya 12.9% ni 5026d.nm. Microemuliyoni ya Tween-20 na Span-60 ivanze ikwirakwizwa isa niy'amata, hamwe na 81.7% ingano ya 5421d.nm na 18.3% ingano ya 180.1d.nm.

3. Kuganira kubisubizo byubushakashatsi

Kuri emulisiferi yo gutegura microemuliyoni ihindagurika, akenshi nibyiza gukoresha ikomatanya rya hydrophilique surfactant na lipophilic surfactant. Ibi ni ukubera ko solubile ya surfactant imwe muri sisitemu iba mike. Nyuma yibi byombi bimaze guhuzwa, Amatsinda ya hydrophilique nandi matsinda ya lipofilique arafatanya kugirango bigire ingaruka. Agaciro ka HLB nako gakunze gukoreshwa mugihe uhitamo emulisiferi. Muguhindura agaciro ka HLB, igipimo cyibice bibiri bigize emulisiferi irashobora gutezimbere, kandi microsperes imwe irashobora gutegurwa. Muri ubu bushakashatsi, hakoreshejwe lipofilique Span-60 (HLB = 4.7) na hydrophilique Tween-20 (HLB = 16.7) yakoreshejwe nk'ikwirakwiza, naho Span-20 yakoreshejwe yonyine nk'iyitatanya. Uhereye kubisubizo byubushakashatsi, birashobora kugaragara ko ifumbire Ingaruka iruta imwe imwe. Microemuliyoni yo gukwirakwiza ibice birasa kandi ifite amata asa; microemuliyoni ikoresheje ikwirakwiza imwe ifite ubukonje bukabije hamwe nuduce twera. Impinga ntoya igaragara munsi yikwirakwizwa rya Tween-20 na Span-60. Impamvu ishoboka nuko impagarara zintera za sisitemu yo guhuza Span-60 na Tween-20 ari ndende, kandi ikwirakwiza ubwayo iracika munsi yubushyuhe bukabije butera gukora Ibice byiza bizagira ingaruka kubisubizo byubushakashatsi. Ingaruka zo gutatanya Tween-20 ni uko ifite umubare munini wiminyururu ya polyoxyethylene (n = 20 cyangwa irenga), bigatuma inzitizi zikomeye hagati ya molekile ya surfactant iba nini kandi biragoye kuba yuzuye kuri interineti. Urebye guhuza ibishushanyo mbonera by'ibice, ibice byera imbere bishobora kuba selile idasobanutse. Kubwibyo, ibisubizo byubu bushakashatsi byerekana ko ingaruka zo gukoresha imiti ikwirakwiza ari nziza, kandi ubushakashatsi bushobora kurushaho kugabanya ingano ya Tween-20 kugirango microsperes zateguwe zirusheho kuba imwe.

Byongeye kandi, amakosa amwe mumikorere yubushakashatsi agomba kugabanywa, nko gutegura hydroxide ya sodium mugikorwa cyo gusesa HPMC, kugabanuka kwa DVS, nibindi, bigomba gushyirwaho uburyo bushoboka kugirango hagabanuke amakosa yubushakashatsi. Ikintu cyingenzi cyane ni ingano yo gutatanya, umuvuduko nimbaraga zo gukangura, nubunini bwumukozi uhuza. Gusa iyo igenzuwe neza irashobora gutegurwa microsperes ya hydrogel hamwe no gutatanya neza hamwe nubunini bumwe.