Gufata bagiteri selile nkibikoresho fatizo, synthesize 2-hydroxy-3-sulfate propyate selulose ether. Infrared spectrometer isesengura imiterere yibicuruzwa. Uburyo bwiza bwo gutunganya synthèse ya bacteri selile selile ether. Ibisubizo byerekanye ko ubushobozi bwo guhanahana 2-hydroxy-3-sulfonique aside ishingiye kuri propyate bacterial ether ikomatanyirizwa hamwe mubihe byari byiza 0.481mmol / g.
Ijambo ryibanze: selile selile; 2-hydroxyl-3-sulfonic aside ishingiye kuri gornemine selulose ether; ubushobozi bwo guhana
Microbial synthique bacterial selulose isa na selile selile yibimera bigize imiterere ya molekile. Ni polysaccharide igororotse ihujwe na D-pyrarot glucose hamweβ-1, 4-glycoside. Ugereranije na selile selile, bagiteri selile ifite ibyiza biranga. Ni ultra -micro fibre net igizwe na fibre ultra -micro. Ibaho muburyo bwa selile nziza kandi ifite imirimo myinshi idasanzwe. Ibice by'ibikoresho bya acoustic no gucukura peteroli byakoreshejwe cyane.
2-hydroxyl-3-sulfonate selile selulose ether ninkomoko yingenzi ya selile ishobora gukorwa mubikoresho byo kwinjiza amazi menshi. Irashobora kandi gukoreshwa nkisuku ihamye yo kwinjiza ibyuma biremereye ion na proteyine nka cation. Feng Qingqin, Jie Zhefeng hamwe na selile yandi akoreshwa mumashanyarazi y'ibigori yumuceri kugirango bategure 2-hydroxyl-3-sulfate selulose ether ikomeye ya acide cationic. Iyi ngingo ikoresha bacteri selulose nkibikoresho fatizo, ikomatanya 2-hydroxyl-3-sulfonic aside ishingiye kuri bacteri selulose ether, kandi ikoresha ubushakashatsi bwa orthogonal kugirango yige imiterere yubukorikori bwiza na 2-hydroxyl-3-sulfa-sulfa sulfa yateguwe muri ubu buryo. Ubushobozi bwo guhanahana aside-ishingiye kuri gornemine selulose ether itanga ishingiro ryukuri kubikorwa bifatika.
1. Igice cyubushakashatsi
1.1 Ibikoresho n'ibikoresho
Selulose ya bagiteri (yakozwe wenyine), sodium hydroxide, sodium karubone, sodium bisulfite, dioxane, epichlorohydrin, acetone, etanol, karubone ya sodium, reagent yavuzwe haruguru ni murwego rwo gusesengura.
Inkubator / yumisha agasanduku (Shanghai-Heng Technology Co., Ltd.); Uruganda rw'indege rwa GQF-1 (Powder Centre, kaminuza ya siyansi n'ikoranabuhanga ya Nanjing); Fourier infrared spectrometer (Ubudage); Agilent AAS-3510 atomic absorption spectrophotometer.
1.2 Gutegura 2-hydroxy-3-sulfopropyl bacterial selulose ether
1.2.1 Synthesis ya bagiteri ihuza selile
Ongeramo 10g ya puderi ya bacteri selile, 60mL ya epichlorohydrin na 125mL ya 2mol·L-1 NaOH igisubizo mumashanyarazi yamajosi atatu afite kondenseri ya flux na stirrer, ubushyuhe bwo kugaruka kuri 1h, kuyungurura, no gukaraba hamwe na acetone namazi kumitungo iciriritse, hanyuma akumishwa munsi yumuvu kuri 60°C kugirango ubone bagiteri ihuza selile.
1.2.2 Synthesis ya sodium 3-chloro-2 hydroxypropanesulfonate
Gupima 104.0gNaHSO3 hanyuma uyishongeshe muri 200mLH2O, hanyuma ureke yuzuze gaze ya SO2. Shyushya kugeza 70-90°C hamwe no gukurura, hanyuma ongeramo 160mL epichlorohydrin hamwe na feri ya feri, hanyuma ukore kuri 85°C kuri 4h. Igicuruzwa cya reaction cyakonje kugeza munsi ya 5°C gutondekanya ibicuruzwa, hanyuma guswera kuyungurura, gukaraba, no gukama kugirango ubone ibicuruzwa byumuhondo byijimye. Igicuruzwa cyibanze cyongeye gushyirwaho na 1: 1 Ethanol kugirango ibone kristu yera.
1.2.3 Synthesis ya 2-hydroxy-3-sulfopropyl bacterial selulose ether
Ongeramo g 2 ya bacteri selile ihuza selile, umubare munini wa 3-chloro-2-hydroxypropanesulfonate, 0,7 g ya karubone ya sodium, na 70 mL yumuti wamazi wa dioxane mumazi atatu yiziritse hamwe na kondenseri hamwe na stirrer, azote Mu kurinda, igenzura ubushyuhe runaka hanyuma ukangure kugira icyo ukora mugihe runaka, kuyungurura, gukaraba hamwe na acetone namazi kugirango ubogamye, na vacuum-yumye kuri 60°C kugirango ubone umuhondo woroshye.
1.3 Isesengura ryimiterere yibicuruzwa
Ikizamini cya FT-IR: tablet ikomeye ya KBr, igipimo cyibizamini: 500cm-1～4000cm-1.
1.4 Kugena ubushobozi bwo guhanahana amakuru
Fata 1-2g ya 2-hydroxy-3-sulfopropyl bacterial selulose ether, ongeramo umubare ukwiye wamazi yatoboye kugirango ushire, hanyuma uyasuke mumurongo wo guhanahana ukoresheje, ukarabe n'amazi akwiye, hanyuma ukoreshe hafi 100mL 5% Hydrochloric aside yoge, igenzure umuvuduko wa 3mL kumunota. Noneho kwoza n'amazi yatoboye kugeza igihe aterekanye acide mugihe ugeragejwe na methyl orange, hanyuma ukureho hafi 60mL ya sodium chloride hamwe na 1mol L-1, ugenzure umuvuduko wa 3mL / min, hanyuma ukusanyirize imyanda hamwe na Erlenmeyer flask. Noneho kwoza inkingi n'amazi ya 50-80mL. Igisubizo cyakusanyirijwe hamwe cyanditseho 0.1mol·L-1 sodium hydroxide igisubizo gisanzwe ukoresheje phenolphthalein nkikimenyetso, kandi umubare wa mililitiro ya sodium hydroxide yakoreshejwe ni VNaOH.
2. Ibisubizo n'ibiganiro
2.1 Imiterere iranga bacteri selile ihuza selile
Bitewe no kumenyekanisha C.-H, bacteri selile ihuza selile ni 2922.98cm-1. Kuzunguruka kurambuye kwa C.-H ku mpeta y'isukari yongerewe imbaraga, kandi impanuro yo kwinjiza amatsinda ya hydroxyl kuri 1161.76cm-1 na 1061.58cm-1 y'umurongo wa spécran a a intege nke, zikaba arizo mpinga zo kwinjiza amatsinda ya hydroxyl muri selile. Kuri 3433.2cm-1, impinga yo kwinjiza vibrational yo mumatsinda ya hydroxyl ifitanye isano iracyahari, ariko ubukana bugereranije buragabanuka, byerekana ko itsinda rya hydroxyl kumpeta ya glucoside ritigeze risimburwa rwose.
2.2 Ibiranga imiterere ya sodium 3-chloro-2-hydroxypropanesulfonate
3525～3481cm-1 ni ihindagurika rirambuye ryishyirahamwe hydroxyl O.-H inkwano, 2930.96cm-1 ni ihindagurika rya asimmetrike irambuye ya C.-H, 2852.69cm ni ihindagurika rirambuye rya C.-H, 1227.3cm-1, 1054.-Cl, byerekana ko ibicuruzwa bigenewe byakozwe.
2.3 Ibiranga imiterere ya 2-hydroxy-3-sulfopropyl bacterial selulose ether
34. URUBUGA Rurambuye kunyeganyega.
2.4 Gukwirakwiza imiterere ya synthesis ya 2-hydroxy-3-sulfopropyl bacterial selulose ether
Mu bushakashatsi, ubushobozi bwo guhanahana amakuru bwakoreshejwe mu gupima ubuziranenge bwa 2-hydroxy-3-sulfopropyl bacterial selulose ether. Ingano ya 3-chloro-2 hydroxypropanesulfonate sodium yongewemo mubitekerezo, kwibumbira hamwe kwamazi yo mu mazi ya dioxane, igihe cyo kubyitwaramo nubushyuhe bwakoze ibintu bine hamwe ninzego eshatu zubushakashatsi bwa orthogonal kugirango isesengure ingaruka za buri kintu kuri bacteri selulose xanthate. . Ingaruka z'umutungo wa ester.
Ubushakashatsi bwa orthogonal bwerekana ko guhuza neza ibintu 4 ari A2B1C3D. 1 Isesengura ryerekana ko ubushyuhe bwa reaction bugira uruhare runini kumikorere ya adsorption ya 2-hydroxy-3-sulfopropyl selulose ether, kandi intera ni 1. 914, ikurikirwa no kwibanda kumwanya, dioxyde, no kugaburira 3 -chloro-2 hydroxypropanesulfonate sodium. Ubushobozi bwo guhanahana 2-hydroxy-3-sulfopropyl bacterial selulose ether yateguwe mubihe byiza byari 0.481mmol / g, ibyo bikaba byari hejuru yubwa SE-selile yo mu bwoko bwa SE-selile ikomeye yo guhana ibiti bivugwa mu gitabo.
3. Umwanzuro
Muguhindura selile ya bagiteri, 2-hydroxy-3-sulfonic acide propyl bacterial selulose ether yarashizwemo, kandi imiterere yaranzwe irapimwa ubushobozi bwo guhanahana amakuru. Hafashwe imyanzuro ikurikira: 1) 2-hydroxy-3 - Uburyo bwiza bwo gutunganya uburyo bwa synthesis ya bacteri ya sulfopropyl selulose ether ni: 2g ihuza bagiteri selile selile, 3.5g 3-chloro-2-hydroxypropanesulfonate sodium, 0,7g sodium karubone na 7OmI30% dioxane Igisubizo cyamazi, reaction kuri 70°C mukurinda azote kuri 1h, 2-hydroxy-3-sulfonic acide propyl bacterial selulose ether yateguwe muriki kibazo ifite ubushobozi bwo guhanahana amakuru; )
Igihe cyo kohereza: Werurwe-06-2023