Nkwadebe nke Hydrogel Microspheres sitere na Hydroxypropyl Methyl Cellulose

Nnwale a na-anabata usoro nkwusioru nkwusioru nke oge polymerization, na-eji hydroxypropyl methylcellulose (HPMC) dị ka akụrụngwa, sodium hydroxide ngwọta dị ka mmiri mmiri, cyclohexane dị ka mmanụ mmanụ, na divinyl sulfone (DVS) dị ka cross-njikọ ngwakọta nke Tween- 20 na Span-60 dị ka onye na-ekesa, na-akpali na ọsọ nke 400-900r / min iji kwadebe microspheres hydrogel.

Isi okwu: hydroxypropyl methylcellulose; hydrogel; microspheres; na-ekesa

1.Nchịkọta

1.1 Nkọwa nke hydrogel

Hydrogel (Hydrogel) bụ ụdị polymer molekụla dị elu nke nwere nnukwu mmiri n'ime usoro netwọkụ na-adịghị edozi ya na mmiri. A na-ewebata akụkụ nke otu hydrophobic na hydrophilic residues n'ime polymer soluble mmiri nwere usoro njikọ njikọ netwọk, na hydrophilic The residues na-ejikọta na mmiri mmiri, na-ejikọta mkpụrụ ndụ mmiri n'ime netwọk ahụ, ebe ihe fọdụrụ na hydrophobic na-ejupụta na mmiri na-emepụta obe. - njikọ polymers. Jellies na lenses kọntaktị na ndụ kwa ụbọchị bụ ngwaahịa hydrogel niile. Dị ka size na ọdịdị nke hydrogel, ọ nwere ike kewaa macroscopic gel na microscopic gel (microsphere), na mbụ nwere ike kewaa columnar, porous sponge, fibrous, membranous, spherical, wdg The ugbu a kwadebere microspheres na nanoscale microspheres. nwere ịdị nro dị mma, ngbanwe, ikike nchekwa mmiri na biocompatibility, ma jiri ya mee nchọpụta nke ọgwụ ndị a na-etinye n'ime.

1.2 Mkpa nhọrọ isiokwu

N'ime afọ ndị na-adịbeghị anya, iji mezuo ihe ndị a chọrọ maka nchebe gburugburu ebe obibi, ihe ndị polymer hydrogel ejiriwo nwayọọ nwayọọ dọta uche zuru ụwa ọnụ n'ihi na ha dị mma hydrophilic Njirimara na biocompatibility. A kwadebere microspheres Hydrogel site na hydroxypropyl methylcellulose dị ka akụrụngwa na nnwale a. Hydroxypropyl methylcellulose bụ ihe na-abụghị ionic cellulose ether, na-acha ọcha ntụ ntụ, na-esi ísì ụtọ na-enweghị ụtọ, na nwere irreplaceable e ji mara ndị ọzọ sịntetik polymer ihe, n'ihi ya, o nwere nnukwu nnyocha uru na polymer ubi.

1.3 Ọnọdụ mmepe n'ụlọ na mba ọzọ

Hydrogel bụ ụdị usoro onunu ogwu nke dọtara mmasị nke ukwuu na obodo ahụike mba ụwa n'afọ ndị na-adịbeghị anya ma tolite ngwa ngwa. Ebe Wichterle na Lim bipụtara ọrụ ọsụ ụzọ ha na HEMA hydrogels cross-linked na 1960, nyocha na nyocha nke hydrogels anọgidewo na-amụbawanye. N'etiti 1970s, Tanaka chọpụtara pH-sensitive hydrogels mgbe ọ na-atụle nha nha nke acrylamide gels merela agadi, na-akara akara ọhụrụ n'ime ọmụmụ nke hydrogels. obodo m nọ na ogbo mmepe hydrogel. N'ihi usoro nkwadebe dị ukwuu nke ọgwụ ndị China omenala na ihe mgbagwoju anya, ọ na-esiri ike wepụ otu ngwaahịa dị ọcha mgbe ọtụtụ ihe na-arụkọ ọrụ ọnụ, na usoro ọgwụgwọ dị ukwuu, ya mere mmepe nke hydrogel ọgwụ ndị China nwere ike ịdị nwayọọ.

1.4 Ihe nnwale na ụkpụrụ

1.4.1 Hydroxypropyl methylcellulose

Hydroxypropyl methyl cellulose (HPMC), ihe sitere na methyl cellulose, bụ ihe dị mkpa agwakọta ether, nke bụ nke na-abụghị ionic mmiri-soluble polymers, na-enweghị isi, enweghị ụtọ na-adịghị egbu egbu.

Industrial HPMC bụ n'ụdị ọcha ntụ ntụ ma ọ bụ na-acha ọcha rụrụ eriri, na aqueous ngwọta nwere elu ọrụ, elu nghọta na mụ arụmọrụ. N'ihi na HPMC nwere ihe onwunwe nke thermal gelation, ngwaahịa aqueous ngwọta na-ekpo ọkụ na-etolite a gel na precipitates, wee dissolves mgbe obi jụrụ, na gelation okpomọkụ nke dị iche iche nkọwa nke ngwaahịa dị iche iche. Njirimara nke nkọwapụta dị iche iche nke HPMC dịkwa iche. Solubility na-agbanwe na viscosity na uru pH adịghị emetụta ya. Na ala viscosity, ka ukwuu solubility. Ka ọdịnaya nke methoxyl na-ebelata, ebe gel nke HPMC na-abawanye, mmiri solubility na-ebelata, ọrụ elu na-ebelata. N'ime ụlọ ọrụ biomedical, a na-ejikarị ya dị ka ihe polymer na-achịkwa ọnụego maka ihe mkpuchi, ihe nkiri, na nkwado nkwado nkwado. Enwere ike iji ya dị ka ihe nkwụsi ike, ihe nkwụsịtụ, nrapado mbadamba, na nkwalite viscosity.

1.4.2 Ụkpụrụ

N'iji usoro nkwusioru nke nkwusioru nke usoro polymerization, na-eji Tween-20, Span-60 compound dispersant na Tween-20 dị ka ndị na-ekesa dị iche iche, chọpụta uru HLB (surfactant bụ amphiphile na ìgwè hydrophilic na lipophilic Group Molecule, ọnụ ọgụgụ nke nha na ike). A na-akọwa nguzozi n'etiti ìgwè hydrophilic na lipophilic group na surfactant molecule dị ka nso nso nke hydrophilic-lipophilic itule uru nke surfactant na-eji dị ka mmanụ mmanụ Cyclohexane nwere ike ka mma gbasasịa monomer ngwọta na igbasa okpomọkụ eme na nnwale na-aga n'ihu na usoro onunu ogwu bụ 1-5 ugboro nke monomer aqueous ngwọta Na a ịta nke 99% divinyl sulfone dị ka cross-njikọ gị n'ụlọnga, na ego nke cross-njikọ mmadụ na-achịkwa na banyere 10% nke. na akọrọ cellulose uka, nke mere na otutu linear ụmụ irighiri na-njikọ na onye ọ bụla ọzọ na cross-njikọ n'ime a netwọk Ọdịdị A ihe na covalently agbụ ma ọ bụ mmemmem ma ọ bụ ionic njikọ n'etiti polymer molekul chains.

Mkpali dị ezigbo mkpa maka nnwale a, a na-achịkwa ọsọ ọsọ na ngwa nke atọ ma ọ bụ nke anọ. N'ihi na nha nke ntụgharị ntụgharị na-emetụta kpọmkwem nha nke microspheres. Mgbe ọsọ ntụgharị dị ukwuu karịa 980r / min, a ga-enwe nnukwu ihe na-ejide mgbidi, nke ga-ebelata oke ngwaahịa ngwaahịa; Onye na-emekọrịta ihe na-eme ka ọ na-emepụta nnukwu gels, na enweghị ike ịnweta ngwaahịa gburugburu.

2. Ngwa na ụzọ nnwale

2.1 Ngwa nnwale

Eletrọnịkị itule, multifunctional eletriki mkpagharị, polarizing microscope, Malvern urughuru size analyzer.

Iji kwadebe cellulose hydrogel microspheres, isi kemịkalụ na-eji bụ cyclohexane, Tween-20, Span-60, hydroxypropyl methylcellulose, divinyl sulfone, sodium hydroxide, distilled mmiri, ihe niile Monomers na mgbakwunye na-eji ozugbo na-enweghị ọgwụgwọ.

2.2 Nkwadebe nke cellulose hydrogel microspheres

2.2.1 Iji Tween 20 dị ka onye na-ekesa

Mgbasa nke hydroxypropylmethylcellulose. Were nha nke ọma 2g nke sodium hydroxide wee kwadebe ngwọta sodium hydroxide 2% yana flask volumetric 100ml. Were 80ml nke ngwọta sodium hydroxide a kwadebere ma kpoo ya n'ime mmiri ịsa mmiri ruo ihe dịka 50°C, tụọ 0.2g nke cellulose ma gbakwunye ya na ngwọta alkaline, kpalite ya na mkpanaka iko, tinye ya na mmiri oyi maka ịsa mmiri ice, ma jiri ya dị ka mmiri mmiri mgbe edozichara ngwọta ahụ. Jiri cylinder gụsịrị akwụkwọ iji tụọ 120ml nke cyclohexane (mmanụ mmanụ) n'ime flask atọ nwere olu, dọta 5ml nke Tween-20 n'ime mmanụ mmanụ na sirinji, ma kpalie na 700r / min maka otu awa. Were ọkara nke usoro mmiri a kwadebere ma tinye ya na flask olu atọ ma kpalie ruo awa atọ. Ntụle nke divinyl sulfone bụ 99%, gbazere na 1% na mmiri distilled. Jiri pipette were 0.5ml nke DVS n'ime 50ml volumetric flask iji dozie 1% DVS, 1ml nke DVS bụ 0.01g. Jiri pipette were 1ml banye n'olu atọ. Tinye ọkụ n'ime ụlọ maka awa 22.

2.2.2 Iji span60 na Tween-20 dị ka ndị na-ekesa

Ọkara nke ọzọ nke usoro mmiri nke edoziworo. Na-atụ 0.01gspan60 ma gbakwunye ya na tube ule, kpoo ya na mmiri mmiri dị ogo 65 ruo mgbe ọ gbazee, wee tinye obere cyclohexane ole na ole n'ime mmiri ịsa ahụ na mmiri rọba, ma kpoo ya ruo mgbe ngwọta ahụ na-acha ọcha. Tinye ya na iko olu atọ, wee tinye 120ml nke cyclohexane, kpochaa tube ule na cyclohexane ọtụtụ ugboro, kpoo ọkụ maka 5min, dị jụụ n'ime ụlọ, tinye 0.5ml nke Tween-20. Mgbe emechara maka awa atọ, a na-agbakwunye 1ml nke DVS diluted. Tinye ọkụ n'ime ụlọ maka awa 22.

2.2.3 Nsonaazụ nnwale

A na-etinye ihe nlele ahụ a kpalitere n'ime mkpanaka iko wee gbazee na 50ml nke ethanol zuru oke, ma tụọ nha nha ahụ n'okpuru nha nke Malvern. Iji Tween-20 dị ka a dispersant microemulsion bụ thicker, na tụrụ urughuru size nke 87.1% bụ 455.2d.nm, na urughuru size nke 12.9% bụ 5026d.nm. The microemulsion nke Tween-20 na Span-60 agwakọta dispersant yiri nke mmiri ara ehi, na 81.7% urughuru size nke 5421d.nm na 18.3% urughuru size nke 180.1d.nm.

3. Mkparịta ụka nke nsonaazụ nnwale

Maka emulsifier maka ịkwadebe microemulsion inverse, ọ na-aka mma iji ngwakọta nke surfactant hydrophilic na lipophilic surfactant. Nke a bụ n'ihi na solubility nke otu surfactant na usoro dị ala. Mgbe emechara nke abụọ ahụ, otu hydrophilic nke ọ bụla na ndị otu lipophilic na-akwado ibe ha ka ha nwee mmetụta solubilizing. Uru HLB bụkwa ihe ndeksi a na-ejikarị eme ihe mgbe ị na-ahọrọ emulsifiers. Site n'ịgbanwe uru HLB, oke nke emulsifier nwere akụkụ abụọ nwere ike imeziwanye, enwere ike ịkwado microspheres ndị ọzọ edo edo. N'ime nnwale a, lipophilic Span-60 (HLB = 4.7) na hydrophilic Tween-20 (HLB=16.7) na-eji dị ka onye na-ekesa, na Span-20 na-eji naanị ya dị ka onye na-ekesa. Site na nsonaazụ nnwale, enwere ike ịhụ na ogige ahụ Mmetụta dị mma karịa otu onye na-ekesa. The microemulsion nke onyinye dispersant bụ dịtụ edo na nwere a mmiri ara ehi-dị ka agbanwe agbanwe; na microemulsion iji otu dispersant nwere oke viscosity na ọcha ahụ. Obere elu na-apụta n'okpuru ihe nkesa nke Tween-20 na Span-60. Ihe nwere ike ime bụ na esemokwu interfacial nke usoro nhazi nke Span-60 na Tween-20 dị elu, na onye na-ekesa ya n'onwe ya na-agbaji n'okpuru nnukwu ike na-akpali akpali na-emepụta ihe ndị dị mma ga-emetụta nsonaazụ nnwale. Ọdịmma nke dispersant Tween-20 bụ na o nwere ọnụ ọgụgụ buru ibu nke polyoxyethylene chains (n ​​= 20 ma ọ bụ otú), nke na-eme ka stric mgbochi n'etiti surfactant ụmụ irighiri ibu na ọ na-esi ike ịbụ ok na interface. Na-ekpe ikpe site na Nchikota nke urughuru size eserese, na-acha ọcha ahụ n'ime nwere ike undispersed cellulose. Ya mere, nsonaazụ nke nnwale a na-atụ aro na mmetụta nke iji ihe na-ekesa ihe na-emepụta ihe dị mma, na nnwale ahụ nwere ike belata ọnụ ọgụgụ nke Tween-20 iji mee ka microsphere ndị a kwadebere dịkwuo mma.

Tụkwasị na nke a, ekwesịrị ibelata ụfọdụ njehie na usoro ọrụ nnwale, dị ka nkwadebe nke sodium hydroxide na usoro mgbasa nke HPMC, dilution nke DVS, wdg, kwesịrị ịhazi dịka o kwere mee iji belata mmejọ nnwale. Ihe kachasị mkpa bụ ọnụọgụ nke mgbasa ozi, ọsọ ọsọ na ike nke ịkpali, na ọnụ ọgụgụ nke onye na-emekọrịta ihe. Naanị mgbe a na-achịkwa nke ọma ka enwere ike ịkwado microspheres hydrogel nwere mgbasa ozi ọma na nha nha nha.