Te Whakaritenga o nga Mokooru Hydrogel mai i te Hydroxypropyl Methyl Cellulose

Ko tenei whakamatautau ka whakamahi i te tikanga whakakao whakatarewatanga polymerization, ma te whakamahi i te hydroxypropyl methylcellulose (HPMC) hei rauemi mata, te otinga konutai hydroxide hei waahanga wai, te cyclohexane hei waahanga hinu, me te divinyl sulfone (DVS) hei hono-whakawhitinga Rahui o te Waa-rua. 20 me Span-60 hei dispersant, whakaoho i te tere o 400-900r / min ki te whakarite moroiti hydrogel.

kupu matua: hydroxypropyl methylcellulose; hydrogel; moroiti; marara

1.Tirohanga

1.1 Whakamaramatanga o te hydrogel

Ko te Hydrogel (Hydrogel) he momo polymer molecular teitei kei roto te nui o te wai i roto i te hanganga whatunga me te kore e rewa i te wai. Ko tetahi waahanga o nga roopu hydrophobic me nga toenga hydrophilic ka whakauruhia ki roto i te polymer wairewa-wai me te hanganga honohono whatunga, me te hydrophilic Ka herea nga toenga ki nga ngota ngota wai, e hono ana i nga ngota ngota wai i roto i te whatunga, i te wa e pupuhi ana nga toenga hydrophobic ki te wai hei hanga ripeka - polymers hono. Ko nga tiēre me nga arotahi whakapiri i roto i te oranga o ia ra he hua hydrogel katoa. E ai ki te rahi me te ahua o te hydrogel, ka taea te wehea ki roto i te kiri macroscopic me te miihini miihini (microsphere), a ka taea te wehea te mua ki te poupou, hautai porous, fibrous, membranous, spherical, me etahi atu. he pai te ngawari, te elasticity, te kaha rokiroki wai me te biocompatibility, ka whakamahia ki te rangahau o nga raau taero kua mau.

1.2 Te hiranga o te whiriwhiri kaupapa

I nga tau tata nei, ki te whakatutuki i nga whakaritenga o te whakamarumaru taiao, ko nga rauemi polymer hydrogel kua kaha te aro nui ki te aro nui na te mea he pai nga ahuatanga hydrophilic me te pai o te koiora. I whakaritea nga moroiti hydrogel mai i te hydroxypropyl methylcellulose hei rauemi mata o tenei whakamatautau. Ko te Hydroxypropyl methylcellulose he ether cellulose kore-ionic, he paura ma, he kakara me te reka, he ahua kore e taea te whakakapi o etahi atu rauemi polymer waihanga, na reira he nui te uara rangahau i roto i te mara polymer.

1.3 Te mana whakawhanake i te kainga me te whenua o waho

Ko te Hydrogel he momo inenga rongoa kua aro nui ki te hapori rongoa o te ao i enei tau kua pahure ake nei, kua tere te tipu. Mai i te wa i whakaputahia e Wichterle raua ko Lim a raua mahi paionia mo te HEMA cross-linked hydrogels i te tau 1960, kua hohonu haere tonu te rangahau me te torotoro i nga hydrogels. I waenganui o te tekau tau atu i 1970, i kitea e Tanaka nga wairere-pH-tairongo i te ine i te owehenga pupuhi o nga reera acrylamide tawhito, e tohu ana i tetahi waahanga hou i roto i te rangahau o nga wairere. Ko taku whenua kei te waahi o te whanaketanga hydrogel. Na te nui o te whakaritenga o te rongoa Hainamana tuku iho me nga waahanga uaua, he uaua ki te tango i te hua parakore kotahi ka mahi tahi nga waahanga maha, a he nui te inenga, na reira ka puhoi te whanaketanga o te rongoa Hainamana hydrogel.

1.4 Nga rauemi me nga maapono whakamatautau

1.4.1 Hydroxypropyl methylcellulose

Ko te Hydroxypropyl methyl cellulose (HPMC), he takenga o te methyl cellulose, he etera whakauru nui, no nga polymers wairewa-kore katote, he hongi, he reka, he paitini.

Ko te HPMC Ahumahi te ahua o te paura ma, te muka wetewete ma ranei, a ko tana wairewa wai he mahi mata, he tino marama me te mahi pumau. Na te mea kei a HPMC te taonga o te waiariki, ka whakamahanahia te wairewa wai o te hua ki te hanga i te reera ka heke, ka rewa i muri i te whakamatao, a he rereke te pāmahana o te waiariki o nga whakaritenga rereke o te hua. He rereke ano nga ahuatanga o nga korero rereke o te HPMC. Ka huri te memehatanga ki te pokey me te kore e pa ki te uara pH. Ko te iti o te pokey, ka nui ake te whakarewatanga. I te mea ka heke te ihirangi o te roopu methoxyl, ka piki ake te waahi o te HPMC, ka heke te wairewa wai, ka heke te mahi o te mata. I roto i te ahumahi biomedical, ka whakamahia te nuinga hei rauemi polymer reiti mo te whakakikorua i nga taonga, nga taonga kiriata, me nga whakaritenga tuku pumau. Ka taea hoki te whakamahi hei whakapumau, hei kaiwhakarewa irirangi, hei whakapiri papa, hei whakaniko i te pokey.

1.4.2 Kaupapa

Ma te whakamahi i te tikanga polymerization whakatārewatanga whakamuri, te whakamahi i te Tween-20, te Span-60 dispersant pūhui me te Tween-20 hei dispersants motuhake, whakatauhia te uara HLB (ko te surfactant he amphiphile me te roopu hydrophilic me te roopu lipophilic Molecule, te nui o te rahi me te kaha. Ko te taurite i waenga i te roopu hydrophilic me te roopu lipophilic i roto i te ngota haumanu ka tautuhia ko te awhe tata o te uara toenga hydrophilic-lipophilic o te surfactant ka whakamahia te Cyclohexane hei waahanga hinu ka taea e te Cyclohexane te whakamarara i te otinga monomer me te whakakore i te wera i roto i te whakamatautau tonu Ko te inenga ko te 1-5 nga wa o te wairewa wai monomer Me te kukū o te 99% divinyl sulfone hei kaihoko whakawhiti, me te nui o te kaihoko whakawhiti i te 10% o. te papatipu cellulose maroke, kia herea ai te maha o nga ngota ngota raina ki tetahi ki tetahi, ka hono-whakawhiti ki roto i te hanganga whatunga He matū e herea ana, e whakangawari ana ranei i te hanga hononga katote i waenga i nga mekameka ngota waerau.

He mea nui te whakakorikori ki tenei whakamatautau, a ko te tere te whakahaere i te taputapu tuatoru, tuawha ranei. No te mea ko te rahi o te tere hurihuri e pa ana ki te rahi o nga microspheres. Ka nui ake te tere o te hurihanga i te 980r/min, ka nui te ahua o te piri o te pakitara, ka tino whakaitihia te hua o te hua; Ko te kaihoko hononga-whakawhitinga ka kaha ki te whakaputa i nga ra maha, a kaore e taea te whiwhi hua porohita.

2. Nga taputapu me nga tikanga whakamatautau

2.1 Taputapu Whakamatau

Te toenga hiko, te whakakorikori hiko maha, te karu polarizing, te kaitirotiro rahi matūriki Malvern.

Hei whakarite i nga microspheres cellulose hydrogel, ko nga matū matua e whakamahia ana ko te cyclohexane, Tween-20, Span-60, hydroxypropyl methylcellulose, divinyl sulfone, konutai hydroxide, te wai maturu, ko enei Monomers me nga taapiri ka whakamahia tika me te kore maimoatanga.

2.2 Nga takahanga o te moroiti waiora cellulose

2.2.1 Te whakamahi i waenganui i te 20 hei whakamarara

Te whakakore i te hydroxypropylmethylcellulose. Kia tika te pauna i te 2 karamu o te konutai waiwai ka whakarite i te wairewa konutai waikawa 2% me te ipu 100ml. Tangohia te 80ml o te wairewa konutai waikawa ka whakamahana ki roto i te pati wai ki te 50 pea°C, paunatia te 0.2g o te cellulose ka tapiri atu ki te wairewa kawakore, whakakorikoria ki te rakau karaihe, tuu ki te wai matao mo te pati hukapapa, ka whakamahia hei waahi wai i muri i te whakamaramatanga o te otinga. Whakamahia he rango kua oti te tohu hei ine i te 120ml o te cyclohexane (waa hinu) ki roto i te ipu kaki-toru, toia te 5ml o Tween-20 ki roto i te wahanga hinu me te syringe, ka whakaoho i te 700r/min mo te haora kotahi. Tangohia te haurua o te wahanga wai kua rite, ka tapirihia ki roto i te ipu kaki-toru me te whakaoho mo nga haora e toru. Ko te kukū o te divinyl sulfone he 99%, diluted ki te 1% ki te wai makuku. Whakamahia he paipa hei tango i te 0.5ml o te DVS ki roto i te ipu volumetric 50ml hei whakarite i te 1% DVS, he rite te 1ml o te DVS ki te 0.01g. Whakamahia he pipette hei tango i te 1ml ki roto i te ipu kaki-toru. Oho i te pāmahana rūma mo te 22 haora.

2.2.2 Te whakamahi i te span60 me te Tween-20 hei whakamarara

Ko te haurua o te wahanga wai katahi ano ka rite. Paunatia te 0.01gspan60 ka tāpirihia ki te ipuipu, whakamahana i roto i te kaukau wai 65-tohu kia rewa, katahi ka makahia etahi pata o te cyclohexane ki roto i te pati wai me te topata rapa, ka whakamahana kia huri ra ano te wairewa kia ma. Tāpirihia ki te ipu kakī-toru, kātahi ka tāpirihia te 120ml o te cyclohexane, ka horoia te ipuipu ki te cyclohexane kia maha nga wa, whakamahana mo te 5min, whakamatao ki te pāmahana rūma, ka tapirihia te 0.5ml o Tween-20. I muri i te whakaoho mo nga haora e toru, ka tapirihia te 1ml o te DVS waimeha. Oho i te pāmahana rūma mo te 22 haora.

2.2.3 Nga hua whakamatautau

I toua te tauira i whakakorikoria ki roto i te rakau karaihe ka rewa ki te 50ml o te waihano tino, ka ine te rahi o te matūriki i raro i te rahi o te matūriki Malvern. Ko te whakamahi i te Tween-20 hei microemulsion dispersant he matotoru ake, a ko te rahi o te matūriki o te 87.1% ko te 455.2d.nm, ko te rahi o te matūriki o te 12.9% he 5026d.nm. He rite te microemulsion o Tween-20 me Span-60 ki te miraka, he 81.7% te rahi matūriki o te 5421d.nm me te 18.3% te rahi o te matūriki o te 180.1d.nm.

3. Te matapaki mo nga hua whakamatautau

No te emulsifier no te faaineine i te microemulsion inverse, e mea maitai a‘e ia faaohipa i te pueraa o te hydrophilic surfactant e te lipophilic surfactant. Ko tenei na te mea he iti te memehatanga o tetahi surfactant kotahi i roto i te punaha. Whai muri i te whakakotahitanga o nga mea e rua, ka mahi tahi nga roopu hydrophilic me nga roopu lipophilic o tetahi ki tetahi ki te whai i te mana whakarewa. Ko te uara HLB he tohu e whakamahia nuitia ana i te wa e whiriwhiri ana i nga whakaemi. Na roto i te whakatikatika i te uara HLB, ka taea te whakatika i te ōwehenga o te whakaemulsifier pūhui e rua-waahanga, ka taea te whakarite i nga microspheres rite. I roto i tenei whakamatautau, he ngoikore te lipophilic Span-60 (HLB = 4.7) me te hydrophilic Tween-20 (HLB = 16.7) i whakamahia hei dispersant, me te Span-20 i whakamahia anake hei dispersant. Mai i nga hua whakamatautau, ka kitea he pai ake te puhui Ko te paanga ki te marara kotahi. Ko te microemulsion o te dispersant pūhui he ahua rite, he rite tonu te rite ki te miraka; te microemulsion e whakamahi ana i te dispersant kotahi he nui rawa te pokey me nga matūriki ma. Ka puta te tihi iti i raro i te dispersant pūhui o Tween-20 me Span-60. Ko te take pea he nui te awangawanga atanga o te punaha puhui o Span-60 me Tween-20, a ko te dispersant ano ka pakaru i raro i te kaha nui o te whakakorikori ki te hanga Ko nga matūriki pai ka pa ki nga hua whakamatautau. Ko te kino o te dispersant Tween-20 ko te maha o nga mekameka polyoxyethylene (n=20 neke atu ranei), ka nui ake te aukati i waenga i nga ngota ngota surfactant me te uaua ki te kiato ki te atanga. Ki te whakatau i te whakakotahitanga o nga hoahoa rahi matūriki, ko nga matūriki ma o roto pea he cellulose kore e marara. No reira, ko nga hua o tenei whakamatautau he pai ake te painga o te whakamahi i te dispersant pūhui, a ka taea e te whakamatautau te whakaiti ake i te nui o Tween-20 kia pai ake ai te ahua o nga microspheres kua rite.

I tua atu, me whakaiti etahi hapa i roto i te tukanga mahi whakamatautau, penei i te whakarite o te konutai waikawa i roto i te tukanga whakakore o te HPMC, te waimeha o te DVS, me etahi atu, me whakataurite i nga mea ka taea ki te whakaiti i nga hapa whakamatautau. Ko te mea nui ko te nui o te dispersant, te tere me te kaha o te whakaoho, me te nui o te kaihoko whakawhiti. Ma te whakahaere tika ka taea te whakarite i nga microspheres hydrogel me te marara pai me te rahi matūriki rite.