Hoʻomākaukau i nā Microspheres Hydrogel mai Hydroxypropyl Methyl Cellulose

Hoʻohana kēia hoʻokolohua i ke ʻano polymerization hoʻokuʻu ʻana, me ka hoʻohana ʻana i ka hydroxypropyl methylcellulose (HPMC) ma ke ʻano he mea maka, ka solution sodium hydroxide ma ke ʻano o ka wai, cyclohexane ma ke ʻano o ka aila, a me ka divinyl sulfone (DVS) ma ke ʻano o ka hui ʻana o waena o waena. 20 a me Span-60 e like me ka dispersant, e hoʻoulu i ka wikiwiki o 400-900r / min e hoʻomākaukau i nā microspheres hydrogel.

Hua'ōlelo nui: hydroxypropyl methylcellulose; hydrogel; microspheres; dispersant

1.Nānā nui

1.1 Ka wehewehe o ka hydrogel

ʻO Hydrogel (Hydrogel) kahi ʻano polymer molecular kiʻekiʻe i loaʻa ka nui o ka wai i loko o ka ʻōnaehana pūnaewele a hiki ʻole ke hoʻoheheʻe ʻia i ka wai. Hoʻokomo ʻia kahi ʻāpana o nā pūʻulu hydrophobic a me nā koena hydrophilic i loko o ka polymer hiki ke hoʻoheheʻe ʻia i ka wai me kahi ʻōnaehana crosslinked network, a me ka hydrophilic Hoʻopili ʻia nā koena i nā molekole wai, e hoʻopili ana i nā molekole wai i loko o ka pūnaewele, aʻo nā koena hydrophobic e pehu me ka wai e hana kea. - nā polymers pili. ʻO nā jellies a me nā leka pili i ke ola o kēlā me kēia lā nā huahana hydrogel āpau. Wahi a ka nui a me ke ʻano o ka hydrogel, hiki ke hoʻokaʻawale ʻia i loko o ka macroscopic gel a me ka microscopic gel (microsphere), a hiki ke hoʻokaʻawale ʻia ka mua i columnar, porous sponge, fibrous, membranous, spherical, etc. ʻO nā microspheres i hoʻomākaukau ʻia i kēia manawa a me nā microspheres nanoscale he palupalu maikaʻi, elasticity, hiki ke mālama wai a me ka biocompatibility, a hoʻohana ʻia i ka noiʻi ʻana i nā lāʻau i hoʻopaʻa ʻia.

1.2 Koʻikoʻi o ke koho kumuhana

I nā makahiki i hala iho nei, i mea e hoʻokō ai i nā koi o ka mālama ʻana i ke kaiapuni, ua hoʻomāliʻi ʻia nā mea polymer hydrogel ma muli o kā lākou waiwai hydrophilic maikaʻi a me ka biocompatibility. Ua hoʻomākaukau ʻia nā microspheres Hydrogel mai ka hydroxypropyl methylcellulose ma ke ʻano he mea maka i kēia hoʻokolohua. ʻO ka Hydroxypropyl methylcellulose kahi etera cellulose non-ionic, pauka keʻokeʻo, ʻaʻala a me ka ʻono ʻole, a he mau hiʻohiʻona hiki ʻole ke hoʻololi ʻia o nā mea polymer synthetic ʻē aʻe, no laila he waiwai noiʻi kiʻekiʻe ma ka pā polymer.

1.3 Ke kūlana hoʻomohala ma ka home a ma waho

ʻO Hydrogel kahi ʻano lāʻau lāʻau lapaʻau i huki nui ʻia i ke kaiāulu olakino honua i nā makahiki i hala iho nei a ua ulu wikiwiki. Mai ka wā i hoʻopuka ai ʻo Wichterle lāua ʻo Lim i kā lāua hana paionia ma ka HEMA cross-linked hydrogels i ka makahiki 1960, ua hoʻomau ka hohonu o ka noiʻi a me ka ʻimi ʻana i nā hydrogels. I ka waena o 1970s, ua ʻike ʻo Tanaka i nā hydrogels pH-sensitive i ke ana ʻana i ka ratio pehu o nā gels acrylamide kahiko, e hōʻailona ana i kahi hana hou i ke aʻo ʻana i nā hydrogels. Aia koʻu ʻāina i ke kahua o ka hoʻomohala hydrogel. Ma muli o ke kaʻina hana hoʻomākaukau nui o ka lāʻau lapaʻau Kina a me nā mea paʻakikī, paʻakikī ke unuhi i kahi huahana maʻemaʻe hoʻokahi ke hana pū nā ʻāpana he nui, a nui ka dosage, no laila e lohi paha ka ulu ʻana o ka hydrogel lāʻau Kina.

1.4 Mea hoʻokolohua a me nā loina

1.4.1 Hydroxypropyl methylcellulose

Hydroxypropyl methyl cellulose (HPMC), he derivative o ka methyl cellulose, he mea nui i hui pū ʻia, nona nā polymers non-ionic wai-soluble, a he ʻala ʻole, ʻono ʻole a me nā mea ʻawaʻawa.

ʻO ka HPMC ʻOihana ma ke ʻano o ka pauka keʻokeʻo a i ʻole ka fiber keʻokeʻo keʻokeʻo, a ʻo kāna hoʻonā wai i loaʻa i ka hana ili, kiʻekiʻe ka ʻike a me ka hana paʻa. No ka mea, HPMC i ka waiwai o thermal gelation, ka huahana aqueous solution ua wela e hana i ka gel a me ka precipitates, a laila hoʻoheheʻe ma hope o cooling, a me ka ana o ka gelation okoa kiko'ī o ka huahana. ʻOkoʻa nā waiwai o nā kikoʻī like ʻole o HPMC. Hoʻololi ka solubility me ka viscosity a ʻaʻole pili i ka waiwai pH. ʻO ka haʻahaʻa o ka viscosity, ʻoi aku ka nui o ka solubility. Ke emi nei ka maʻiʻo o ka hui methoxyl, piʻi ka helu gel o HPMC, hoʻemi ka solubility i ka wai, a emi ka hana ma luna. I loko o ka ʻoihana biomedical, hoʻohana nui ʻia ʻo ia ma ke ʻano he mea polymer e hoʻomalu i ka helu no nā mea uhi, nā mea kiʻiʻoniʻoni, a me nā hoʻomākaukau hoʻokuʻu mau. Hiki ke hoʻohana ʻia ma ke ʻano he stabilizer, suspending agent, tablet adhesive, a me viscosity enhancer.

1.4.2 Kumu

Ke hoʻohana nei i ke ʻano polymerization hoʻokuʻu hope, me ka hoʻohana ʻana iā Tween-20, Span-60 compound dispersant a me Tween-20 ma ke ʻano he dispersants kaʻawale, e hoʻoholo i ka waiwai HLB (ʻo ka surfactant he amphiphile me ka hui hydrophilic a me ka hui lipophilic Molecule, ka nui o ka nui a me ka ikaika. ʻO ke kaulike ma waena o ka pūʻulu hydrophilic a me ka pūʻulu lipophilic i loko o ka mole surfactant i wehewehe ʻia e like me ke ʻano o ka waiwai kaulike hydrophilic-lipophilic o ka surfactant hoʻohana ʻia ʻo Cyclohexane e hiki ke hoʻopuehu maikaʻi i ka hopena monomer a hoʻopau i ka wela i hana ʻia i ka hoʻokolohua hoʻomau ʻia ʻo 1-5 mau manawa o ka monomer aqueous solution Me ka hoʻopaʻa ʻana o 99% divinyl sulfone ma ke ʻano he mea hoʻohui kea, a ua hoʻomalu ʻia ka nui o ka mea hoʻohui keʻa ma kahi o 10% o. ka nuipaʻa cellulose maloʻo, no laila e hoʻopaʻa ʻia nā molekala laina he nui i kekahi i kekahi a hoʻopaʻa ʻia i loko o kahi ʻano pūnaewele He mea e hoʻopaʻa covalently a hoʻomaʻamaʻa paha i ka hoʻokumu ʻana i nā kaulahao polimer.

He mea koʻikoʻi ka hoʻoulu ʻana i kēia hoʻokolohua, a mālama ʻia ka wikiwiki ma ke kolu a ʻehā paha. No ka mea, pili pono ka nui o ka wikiwiki rotational i ka nui o nā microspheres. Ke ʻoi aku ka wikiwiki o ka hoʻololi ʻana ma mua o 980r/min, e ʻike ʻia nā mea paʻa paʻa paʻa, e hōʻemi loa i ka hua o ka huahana; Hiki i ka mea hoʻopili kea ke hana i nā gels nui, a ʻaʻole hiki ke loaʻa nā huahana spherical.

2. Nā mea hana hoʻokolohua a me nā ʻano hana

2.1 Mea Hana Hoʻokolohua

ʻO ke kaulike uila, ka mea hoʻoulu uila multifunctional, polarizing microscope, Malvern particle size analyzer.

No ka hoʻomākaukau ʻana i nā microspheres hydrogel cellulose, ʻo nā kinikona nui i hoʻohana ʻia ʻo cyclohexane, Tween-20, Span-60, hydroxypropyl methylcellulose, divinyl sulfone, sodium hydroxide, wai distilled, nā Monomers a me nā mea hoʻohui i hoʻohana pololei ʻia me ka ʻole o ka mālama ʻana.

2.2 Nā ʻanuʻu hoʻomākaukau o cellulose hydrogel microspheres

2.2.1 Hoʻohana i waena o 20 ma ke ʻano he dispersant

Hoʻopau i ka hydroxypropylmethylcellulose. E kaupaona pololei i ka 2g o ka sodium hydroxide a e hoʻomākaukau i kahi solution 2% sodium hydroxide me kahi pahu volumetric 100ml. E lawe i 80ml o ka solution sodium hydroxide i hoʻomākaukau ʻia a wela i loko o ka ʻauʻau wai a hiki i ka 50°C, e kaupaona i ka 0.2g o ka cellulose a hoʻohui i ka hopena alkaline, e hoʻoulu iā ia me ke koʻokoʻo aniani, e waiho i loko o ka wai anuanu no ka ʻauʻau hau, a hoʻohana iā ia ma ke ʻano o ka wai ma hope o ka wehewehe ʻana o ka hopena. E hoʻohana i ka pahu puka e ana i 120ml o ka cyclohexane (ʻaila ʻaila) i loko o ka ipu ʻāʻī ʻekolu, e huki i 5ml o Tween-20 i loko o ka ʻaila ʻaila me kahi syringe, a hoʻoulu i 700r/min no hoʻokahi hola. E lawe i ka hapalua o ka māhele wai i hoʻomākaukau ʻia a hoʻohui i loko o kahi hue ʻāʻī ʻekolu a hoʻoulu i ʻekolu hola. ʻO ka manaʻo o ka divinyl sulfone he 99%, hoʻoheheʻe ʻia i ka 1% me ka wai distilled. E hoʻohana i ka pipette e lawe i 0.5ml o DVS i loko o kahi pahu volumetric 50ml e hoʻomākaukau ai i 1% DVS, ua like ka 1ml o DVS me 0.01g. E hoʻohana i ka pipette e lawe i 1ml i loko o ka ipu ʻāʻī ʻekolu. E hoʻoulu i ka lumi wela no 22 mau hola.

2.2.2 E hoʻohana ana i ka span60 a me ka waena-20 ma ke ʻano he dispersants

ʻO ka hapa ʻē aʻe o ka pae wai i hoʻomākaukau ʻia. Kauona i 0.01gspan60 a hookomo i ka paipu hoao, wela ia i loko o ka wai auau 65-degere a hehee wale, alaila, hoolei iho i kekahi mau kulu o ka cyclohexane i loko o ka wai auau me ka pulupulu, a wela ia a hiki i ka waiu keokeo. E hoʻohui i ka pahu ʻāʻī ʻekolu, a laila e hoʻohui i 120ml cyclohexane, holoi i ka paipu hoʻāʻo me ka cyclohexane i nā manawa he nui, wela no 5min, hoʻomaha a hiki i ka lumi wela, a hoʻohui i 0.5ml o Tween-20. Ma hope o ka hoʻoulu ʻana no ʻekolu hola, ua hoʻohui ʻia ka 1ml o DVS i hoʻoheheʻe ʻia. E hoʻoulu i ka lumi wela no 22 mau hola.

2.2.3 Nā hopena hoʻokolohua

Hoʻokomo ʻia ka laʻana i hoʻoulu ʻia i loko o ke koʻokoʻo aniani a hoʻoheheʻe ʻia i loko o 50ml o ka ethanol piha, a ua ana ʻia ka nui o ka ʻāpana ma lalo o ka Malvern particle sizer. ʻO ka hoʻohana ʻana i ka Tween-20 ma ke ʻano he microemulsion dispersant ʻoi aku ka mānoanoa, a ʻo ka nui o ka 87.1% i ana ʻia he 455.2d.nm, a ʻo ka nui o 12.9% ʻo 5026d.nm. Ua like ka microemulsion o Tween-20 a me Span-60 me ka waiu, me 81.7% ka nui o ka 5421d.nm a me ka 18.3% ka nui o ka 180.1d.nm.

3. Kūkākūkā o nā hopena hoʻokolohua

No ka emulsifier no ka hoʻomākaukau ʻana i ka microemulsion inverse, ʻoi aku ka maikaʻi o ka hoʻohana ʻana i ka hui o hydrophilic surfactant a me lipophilic surfactant. ʻO kēia no ka haʻahaʻa o ka solubility o kahi surfactant hoʻokahi i loko o ka ʻōnaehana. Ma hope o ka hoʻohui ʻia ʻana o nā mea ʻelua, hui pū nā pūʻulu hydrophilic a me nā pūʻulu lipophilic kekahi i kekahi e loaʻa kahi hopena solubilizing. ʻO ka waiwai HLB kekahi helu kuhikuhi maʻamau i ke koho ʻana i nā emulsifiers. Ma ka hoʻololi ʻana i ka waiwai HLB, hiki ke hoʻonui ʻia ka ratio o ka emulsifier hui ʻelua, a hiki ke hoʻomākaukau ʻia nā microspheres like ʻole. Ma kēia hoʻokolohua, ua hoʻohana ʻia ka lipophilic Span-60 (HLB = 4.7) a me ka hydrophilic Tween-20 (HLB = 16.7) ma ke ʻano he dispersant, a ua hoʻohana ʻia ʻo Span-20 wale nō e like me ka dispersant. Mai nā hopena hoʻokolohua, hiki ke ʻike ʻia ka hui ʻoi aku ka maikaʻi ma mua o kahi dispersant hoʻokahi. ʻO ka microemulsion o ka dispersant pūhui he ʻano like ʻole a he ʻano like ka waiū; ʻO ka microemulsion me ka hoʻohana ʻana i hoʻokahi dispersant he kiʻekiʻe loa ka viscosity a me nā ʻāpana keʻokeʻo. ʻIke ʻia ka piko liʻiliʻi ma lalo o ka dispersant pūhui o Tween-20 a me Span-60. ʻO ke kumu hiki ke kiʻekiʻe ke ʻano o ka interfacial tension o ka ʻōnaehana hui o Span-60 a me Tween-20, a ua haki ka dispersant ponoʻī ma lalo o ka hoʻoulu ʻana i ka ikaika nui e hoʻopili i nā hopena hoʻokolohua. ʻO ka hemahema o ka dispersant Tween-20, ʻo ia ka nui o nā kaulahao polyoxyethylene (n=20 a i ʻole), ka mea e hoʻonui ai i ka pale steric ma waena o nā molekala surfactant a paʻakikī ke paʻakikī ma ke kikowaena. Ma ka hoʻoholo ʻana mai ka hui pū ʻana o nā kiʻikuhi nui, ʻo nā ʻāpana keʻokeʻo i loko paha he cellulose ʻaʻole i hoʻokaʻawale ʻia. No laila, hōʻike nā hopena o kēia hoʻokolohua ʻoi aku ka maikaʻi o ka hoʻohana ʻana i ka dispersant pūhui, a hiki i ka hoʻokolohua ke hōʻemi hou i ka nui o Tween-20 e hana i nā microspheres i hoʻomākaukau ʻia.

Eia kekahi, pono e hoʻemi ʻia kekahi mau hewa i ka hana hoʻokolohua, e like me ka hoʻomākaukau ʻana o ka sodium hydroxide i ke kaʻina hoʻoheheʻe o HPMC, ka dilution o DVS, a me nā mea ʻē aʻe, pono e hoʻohālikelike ʻia e like me ka hiki ke hōʻemi i nā hewa hoʻokolohua. ʻO ka mea nui loa ka nui o ka dispersant, ka wikiwiki a me ka ikaika o ka hoʻoulu ʻana, a me ka nui o ka mea hoʻopili cross-linking. Ke hoʻomalu pono ʻia hiki ke hoʻomākaukau ʻia nā microspheres hydrogel me ka hoʻopuehu maikaʻi a me ka nui o nā ʻāpana like ʻole.