E lawe i ka cellulose bacteria ma ke ʻano he kumu, hoʻohui i ka 2-hydroxy-3-sulfate propyate cellulose ether. Hoʻopili ka spectrometer infrared i ke ʻano huahana. ʻO nā kūlana kaʻina hana maikaʻi loa no ka synthesis o ka base bacteria cellulose ether. Ua hōʻike nā hopena i ka hiki ke hoʻololi o 2-hydroxy-3-sulfonic acid-based propyate bacterial ether synthesized ma lalo o nā kūlana optimization he 0.481mmol / g.
Hua'ōlelo: cellulose bacteria; 2-hydroxyl-3-sulfonic acid-based gornemine cellulose ether; hiki ke hoololi
Ua like ka microbial synthetic bacterial cellulose me ka mea kanu cellulose i loko o ke kinikini a me ka molecular structure. He polysaccharide pololei ia e pili ana i ka glucose D-pyrarot meβ-1, 4-glycoside mea paa. Ke hoʻohālikelike ʻia me ka cellulose mea kanu, ʻoi aku ka maikaʻi o ka cellulose bacteria. He ʻupena ultra-micro fiber i haku ʻia me nā fibers ultra-micro. Aia ia ma ke ʻano o ka cellulose maʻemaʻe a he nui nā hana kūikawā. Ua hoʻohana nui ʻia nā hiʻohiʻona o nā mea hana acoustic a me ka ʻaila ʻaila.
ʻO 2-hydroxyl-3-sulfonate cellular cellulose ether he mea waiwai nui ka cellulose derivative i hiki ke hana ʻia me nā mea hoʻoheheʻe wai kiʻekiʻe. Hiki ke hoʻohana ʻia ma ke ʻano he maʻemaʻe paʻa no ka adsorption o nā ion metala kaumaha a me ka protein ma ke ʻano he cation. ʻO Feng Qingqin, Jie Zhefeng a me nā cellulose ʻē aʻe i hoʻohana ʻia i ka mauʻu kulina huila raiki e hoʻomākaukau ai i ka 2-hydroxyl-3-sulfate cellulose ether ikaika acid cationic hoʻololi. Ke hoʻohana nei kēia ʻatikala i ka cellulose bacteria ma ke ʻano he kumu, hoʻohui i ka 2-hydroxyl-3-sulfonic acid-based bacterial cellulose ether, a hoʻohana i nā hoʻokolohua orthogonal e aʻo i kona kūlana synthetic maikaʻi loa a me 2-hydroxyl-3-sulfa-sulfa sulfa i hoʻomākaukau ʻia ma lalo o kēia ʻano. ʻO ka hiki ke hoʻololi o ka gornemine cellulose ether e pili ana i ka waikawa e hāʻawi i kumu kumu no ka hoʻohana maoli ʻana o ka mea.
1. Māhele hoʻokolohua
1.1 Reagents a me na mea hana
ʻO ka cellulose bacterial (hana ponoʻī), sodium hydroxide, sodium carbonate, sodium bisulfite, dioxane, epichlorohydrin, acetone, ethanol, sodium carbonate, ʻo nā reagents i luna o ka helu analytical.
Incubator / pahu maloʻo (Shanghai-Heng Technology Co., Ltd.); GQF-1 jet mill (Powder Center, Nanjing University of Science and Technology); Fourier infrared spectrometer (Kelemānia); Agilent AAS-3510 atomic absorption spectrophotometer.
1.2 Hoʻomākaukau i ka 2-hydroxy-3-sulfopropyl bacterial cellulose ether
1.2.1 Hoʻohui ʻia o ka cellulose bacteria i hoʻopili ʻia
E hoʻohui i 10g o ka pauka cellulose bacteria, 60mL o epichlorohydrin a me 125mL o 2mol·L-1 NaOH hoʻonā i loko o ka ipu ʻāʻī ʻekolu i hoʻolako ʻia me ka reflux condenser a me kahi mea hoʻouluulu, wela i ka reflux no 1h, kānana, a holoi keʻa me ka acetone a me ka wai i nā waiwai liʻiliʻi, a maloʻo ma lalo o ka momi ma 60°C no ka loaʻa ʻana o ka cellulose bacteria i hoʻopaʻa ʻia.
1.2.2 Synthesis o ka sodium 3-chloro-2 hydroxypropanesulfonate
Kauona i 104.0gNaHSO3 a hoʻoheheʻe ʻia i 200mLH2O, a hoʻopiha ʻia me ke kinoea SO2. E wela a hiki i 70-90°C me ka hoʻoulu ʻana, a laila e hoʻohui i ka 160mL epichlorohydrin me kahi funnel e hāʻule ana, a hana i ka 85.°C no 4h. Ua hoʻomaha ʻia ka huahana hopena ma lalo o 5°C no ka crystallize i ka huahana, a laila kānana ʻia, holoi ʻia, a hoʻomaloʻo ʻia no ka loaʻa ʻana o ka hua melemele melemele. Ua hana hou ʻia ka hua palaka me 1:1 ethanol e loaʻa ai nā kristal keʻokeʻo.
1.2.3 Hoʻohui ʻia o 2-hydroxy-3-sulfopropyl bacterial cellulose ether
E hoʻohui i 2 g o ka cellulose bacteri cross-linked, kahi nui o 3-chloro-2-hydroxypropanesulfonate, 0.7 g o ka sodium carbonate, a me 70 mL o ka dioxane aqueous solution i loko o kahi ipu ʻāʻī ʻekolu i hoʻolako ʻia me ka reflux condenser a me kahi mea hoʻoulu. nitrogen Ma lalo o ka pale, e hoʻomalu i kekahi mahana a hoʻoulu e hana no kekahi manawa, kānana, holoi me ka acetone a me ka wai i ka neutrality, a maloʻo maloʻo ma 60°C no ka loaʻa ʻana o kahi paʻa melemele māmā.
1.3 Ke kālailai hoʻolālā huahana
Ho'āʻo FT-IR: papa KBr paʻa, ka laulā hoʻāʻo: 500cm-1～4000cm-1.
1.4 Hoʻoholo i ka hiki ke hoʻololi
E lawe i 1-2g o 2-hydroxy-3-sulfopropyl bacterial cellulose ether, e hoʻohui i ka nui kūpono o ka wai i hoʻomaʻemaʻe ʻia e hoʻoheheʻe ʻia, a laila ninini i loko o ke kolamu hoʻololi me ka hoʻoulu ʻana, holoi me ka nui o ka wai i hoʻoheheʻe ʻia, a laila hoʻohana ma kahi o 100mL 5% Holoi waika Hydrochloric, hoʻomalu i ka kahe kahe o 3mL i kēlā me kēia minuke. A laila holoi me ka wai hoʻomaʻemaʻe a hiki i ka ʻike ʻole ʻia o ka ʻakika ke hoʻāʻo ʻia e ka methyl alani, a laila elute me kahi 60mL o ka sodium chloride me kahi ʻano o 1mol L-1, e hoʻomalu i ka kahe kahe ma kahi o 3mL/min, a e hōʻiliʻili i ka effluent me kahi Pahu Erlenmeyer. A laila holoi i ke kolamu me 50-80mL wai hoʻoheheʻe. Hoʻopili ʻia ka hopena i hōʻiliʻili ʻia me ka 0.1mol·L-1 sodium hydroxide solution maʻamau e hoʻohana ana i ka phenolphthalein ma ke ʻano he hōʻailona, a ʻo ka helu o nā milliliters o ka sodium hydroxide i hoʻopau ʻia ʻo VNaOH.
2. Nā hopena a me ke kūkākūkā
2.1 ʻO ke ʻano o ke ʻano o ka cellulose bacteria i hoʻopili ʻia
Ma muli o ka hoʻomaka ʻana o ka C—H, he 2922.98cm-1 ka cellulose bacteria i hoʻopili ʻia. ʻO ka haʻalulu o C—Hoʻonui ʻia ka H ma ke apo kō, a ua nāwaliwali nā hiʻohiʻona absorption peaks o nā pūʻulu hydroxyl ma 1161.76cm-1 a me 1061.58cm-1 o ka laina spectral a, ʻo ia nā hiʻohiʻona absorption peaks o nā pūʻulu hydroxyl i cellulose. Ma ka 3433.2cm-1, e mau ana ka peak absorption vibrational o ka hui hydroxyl e pili ana, akā e emi ana ka ikaika pili, e hōʻike ana ʻaʻole i hoʻololi loa ʻia ka hui hydroxyl ma ke apo glucoside.
2.2 Hōʻikeʻike ʻia o ka sodium 3-chloro-2-hydroxypropanesulfonate
3525～ʻO 3481cm-1 ka haʻalulu lōʻihi o ka hui hydroxyl O—ʻO ka pilina H, ʻo 2930.96cm-1 ka haʻalulu asymmetric o C—ʻO H, 2852.69cm ka haʻalulu kikoo like o C—H, 1227.3cm-1, 1054. ʻO 95cm-1 ka haʻalulu kikoo o S=O, ʻo 810.1cm-1 ka haʻalulu kikoo o COS, a ʻo 727.4cm-1 ka haʻalulu kikoo o C—Cl, e hōʻike ana ua hoʻokumu ʻia ka huahana i hoʻopaʻa ʻia.
2.3 Nā hiʻohiʻona o ka 2-hydroxy-3-sulfopropyl bacterial cellulose ether
ʻO 3431cm-1 ke kiʻekiʻe o ka haʻalulu OH, ʻo 2917cm-1 ka puʻupuʻu haʻalulu CH saturated, ʻo 1656cm-1 ka CC e kau ana i ka vibration peak, 1212 ~ 1020cm-1 ʻo -SO2-antisymmetric a me ka symmetric kikoo vibration, 658cm ʻo ka haʻalulu hoʻopaʻa SO SO.
2.4 Hoʻonui i nā kūlana synthesis no 2-hydroxy-3-sulfopropyl bacterial cellulose ether
I ka hoʻokolohua, ua hoʻohana ʻia ka mana hoʻololi e hoʻāʻo i ka maikaʻi o ka 2-hydroxy-3-sulfopropyl bacterial cellulose ether. ʻO ka nui o ka sodium 3-chloro-2 hydroxypropanesulfonate i hoʻohui ʻia i ka hopena, ka neʻe ʻana o ka dioxane aqueous solution, ka manawa hoʻonā a me ka mahana i hana i ʻehā mau kumu a me ʻekolu pae o nā hoʻokolohua orthogonal e nānā i ka hopena o kēlā me kēia kumu i ka bacterial cellulose xanthate . Ka hopena o nā waiwai ester.
Hōʻike nā hoʻokolohua Orthogonal ʻo ka hui maikaʻi loa o nā kumu 4 ʻo A2B1C3D. 1 Hōʻike ka hōʻike ʻana i ka hopena i ka hopena nui loa i ka hana adsorption o 2-hydroxy-3-sulfopropyl cellulose ether, a ʻo ka laulā he 1. 914, ukali ʻia e ka ʻike o ka manawa, dioxane, a me ka nui o ka hānai ʻana o 3 -chloro-2 hydroxypropanesulfonate sodium. ʻO ka hiki ke hoʻololi o 2-hydroxy-3-sulfopropyl bacterial cellulose ether i hoʻomākaukau ʻia ma lalo o nā kūlana i hoʻopaʻa ʻia ʻo 0.481mmol/g, ʻoi aku ka kiʻekiʻe ma mua o ka SE-type cellulose ikaika cation hoʻololi lāʻau i hōʻike ʻia ma ka manual.
3. Ka hopena
Ma ka hoʻololi ʻana i ka cellulose bacteria, 2-hydroxy-3-sulfonic acid propyl bacterial cellulose ether i synthesized, a ua ʻike ʻia kona ʻano a ua ana ʻia kona hiki ke hoʻololi. Ua unuhi ʻia kēia mau hopena: 1) 2-hydroxy-3 - ʻO nā kūlana kaʻina hana maikaʻi loa no ka synthesis o ka sulfopropyl bacterial cellulose ether: 2g cross-linked bacterial cellulose, 3.5g 3-chloro-2-hydroxypropanesulfonate sodium, 0.7g sodium carbonate a me 7OmI30% dioxane Waiwai, pane ma 70°C ma lalo o ka naikokene pale no 1h, ka 2-hydroxy-3-sulfonic acid propyl bacterial cellulose ether i hoomakaukauia ma lalo o keia kulana, ua oi aku ka mana hoololi; 2) 2-hydroxy-3-sulfonic acid pūʻulu ʻO ka hiki ke hoʻololi o ka propyl bacterial cellulose ether i ʻoi aku ka kiʻekiʻe ma mua o ka mea like SE type cellulose strong acid cation exchange resin i hōʻike ʻia ma ka puke.
Ka manawa hoʻouna: Mar-06-2023