Nazarin kan halayen rheological na konjac glucomannan da tsarin fili na hydroxypropyl methylcellulose

An dauki tsarin fili na konjac glucomannan (KGM) da hydroxypropyl methylcellulose (HPMC) a matsayin abu na bincike, kuma an gudanar da gwaje-gwaje masu tsauri, mita da zazzabi a kan tsarin fili ta hanyar jujjuyawar rheometer. An yi nazarin tasirin juzu'i mai yawa da kuma mahallin fili akan danko da kaddarorin rheological na tsarin fili na KGM/HPMC. Sakamakon ya nuna cewa tsarin tsarin KGM/HPMC ba shi da ruwa na Newtonian, kuma karuwa a cikin ƙananan juzu'i da KGM na tsarin yana rage yawan ruwa na fili kuma yana ƙara danko. A cikin sol state, KGM da HPMC sarƙoƙi na kwayoyin halitta mafi m tsari ta hanyar hydrophobic hulda. Ƙara yawan juzu'in tsarin tsarin da abun ciki na KGM yana da kyau don kiyaye kwanciyar hankali na tsarin. A cikin ƙananan ƙananan ƙananan ƙananan ƙwayoyin cuta, haɓaka abun ciki na KGM yana da amfani ga samuwar gels na thermotropic; yayin da a cikin babban tsarin juzu'i mai girma, haɓaka abun ciki na HPMC yana da amfani ga samuwar gels na thermotropic.

Mabuɗin kalmomi:konjac glucomannan; hydroxypropyl methylcellulose; fili; rheological hali

Ana amfani da polysaccharides na halitta sosai a cikin masana'antar abinci saboda kauri, emulsifying da kaddarorin gelling. Konjac glucomannan (KGM) shine polysaccharide na halitta, wanda ya ƙunshiβ-D-glucose daβ-D-mannose a cikin rabo na 1.6: 1, an haɗa su biyu ta hanyarβ-1,4 glycosidic bonds, a cikin C- Akwai ƙananan adadin acetyl a matsayi na 6 (kimanin 1 acetyl ga kowane ragowar 17). Koyaya, babban danko da ƙarancin ruwa na KGM ruwa mai ruwa yana iyakance aikace-aikacen sa a samarwa. Hydroxypropyl methylcellulose (HPMC) shine propylene glycol ether na methylcellulose, wanda ke cikin ether cellulose maras ionic. HPMC mai yin fim ne, mai narkewar ruwa, kuma ana iya sabuntawa. HPMC yana da low danko da gel ƙarfi a low yanayin zafi, kuma in mun gwada da matalauta aiki yi, amma zai iya samar da in mun gwada da danko m-kamar gel a high yanayin zafi, don haka da yawa samar matakai dole ne a za'ayi a high yanayin zafi, sakamakon high samar da makamashi amfani. Farashin samarwa yana da yawa. Littattafai sun nuna cewa rukunin mannose da ba a maye gurbinsa ba akan sarkar kwayoyin halitta na KGM na iya samar da yanki mai rauni mai alaƙa da haɗin gwiwar hydrophobic tare da ƙungiyar hydrophobic akan sarkar kwayoyin HPMC ta hanyar hulɗar hydrophobic. Wannan tsarin zai iya jinkirta da wani bangare hana thermal gelation na HPMC da runtse gel zazzabi na HPMC. Bugu da ƙari, bisa la'akari da ƙananan kaddarorin na HPMC a ƙananan yanayin zafi, an yi hasashen cewa haɗawa da KGM na iya inganta haɓakar ƙwararrun KGM da haɓaka aikin sarrafa shi. Sabili da haka, wannan takarda za ta gina tsarin haɗin KGM/HPMC don bincika tasirin juzu'in juzu'i mai yawa da kuma mahaɗar mahalli akan kaddarorin rheological na tsarin KGM/HPMC, da kuma ba da ma'anar ka'idar aikace-aikacen tsarin fili na KGM/HPMC. masana'antar abinci.

1. Kayan aiki da hanyoyin

1.1 Materials da reagents

Hydroxypropyl methylcellulose, KIMA CHEMICAL CO., LTD, taro juzu'i 2%, danko 6mPa·s; methoxy taro juzu'i 28% ~ 30%; 7.0% ~ 12% hydroxypropyl

Konjac glucomannan, Wuhan Johnson Konjac Food Co., Ltd., 1 wt% mai ruwa bayani danko≥28000mPa·s.

1.2 Kayan aiki da kayan aiki

MCR92 rotational rheometer, Anton Paar Co., Ltd., Austria; UPT-II-10T ultrapure ruwa inji, Sichuan Youpu Ultrapure Technology Co., Ltd .; AB-50 ma'aunin nazari na lantarki, kamfanin Swiss Mette; LHS-150HC yawan zafin jiki na ruwa mai wanka, Wuxi Huaze Technology Co., Ltd .; JJ-1 Electric Stirrer, Jintan Medical Instrument Factory, Jiangsu.

1.3 Shiri na fili bayani

Auna HPMC da KGM powders tare da wani ma'auni mai haɗawa (rabo mai yawa: 0:10, 3:7, 5:5, 7:3, 10:0), a hankali ƙara su cikin ruwa mai 60.°C ruwa wanka, da dama ga 1.5 ~ 2 h su sa shi tarwatsa ko'ina, da kuma shirya 5 irin gradient mafita tare da jimlar m taro juzu'i na 0.50%, 0.75%, 1.00%, 1.25%, da kuma 1.50%, bi da bi.

1.4 Gwajin rheological Properties na fili bayani

Gwajin juzu'i mai ƙarfi: An auna ma'aunin rheological na KGM/HPMC fili ta hanyar amfani da mazugi na CP50 da farantin karfe, an daidaita rata tsakanin faranti na sama da na ƙasa a 0.1 mm, ma'aunin zafin jiki shine 25.°C, kuma kewayon shear ya kasance 0.1 zuwa 100 s-1.

Binciken matsi (ƙaddamar da yankin viscoelastic na layi): Yi amfani da farantin PP50 don auna yankin viscoelastic na madaidaiciya da ka'idar canjin yanayin KGM/HPMC, saita tazara zuwa 1.000 mm, ƙayyadaddun mitar zuwa 1Hz, da auna zafin jiki zuwa 25°C. Matsakaicin iyaka shine 0.1% ~ 100%.

Shake mitar: Yi amfani da farantin PP50 don auna canjin yanayin da kuma dogaro da mitar maganin mahadi na KGM/HPMC. An saita tazara zuwa 1.000 mm, nau'in shine 1%, ma'aunin zafin jiki shine 25.°C, kuma kewayon mitar shine 0.1-100 Hz.

Ana bincika yanayin zafin jiki: Modules da yanayin zafin sa na maganin KGM/HPMC an auna ta amfani da farantin PP50, an saita tazara zuwa 1.000 mm, ƙayyadaddun mitar ya kasance 1 Hz, nakasar ta kasance 1%, kuma zazzabi ya kasance daga 25 zuwa 90°C.

2. Sakamako da Nazari

2.1 Binciken lankwasa mai gudana na tsarin mahaɗan KGM/HPMC

Danko mai juzu'i tare da magudanar shear na mafita na KGM/HPMC tare da ma'auni daban-daban na haɗewa a sassa daban-daban na taro. Ruwan da ɗankowarsu aikin layi ne na ƙimar shear ana kiran su ruwan Newtonian, in ba haka ba ana kiran su ruwan da ba na Newtonian ba. Ana iya gani daga lankwasa cewa danko na KGM bayani da KGM / HPMC fili bayani yana raguwa tare da karuwa mai girma; mafi girman abun ciki na KGM, mafi girman juzu'in tsarin taro, kuma mafi bayyananniyar yanayin bakin ciki mai ƙarfi na maganin. Wannan yana nuna cewa KGM da KGM/HPMC tsarin fili ba ruwan Newton bane, kuma nau'in ruwa na tsarin fili na KGM/HPMC yawanci ya ƙaddara ta KGM.

Daga ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun hanyoyin KGM/HPMC tare da ɓangarorin taro daban-daban da ma'auni daban-daban, ana iya ganin cewa ƙimar n na KGM, HPMC da KGM/HPMC fili tsarin duk sun kasance ƙasa da 1, yana nuna cewa mafita shine mafita. duk ruwan pseudoplastic. Don tsarin hadadden tsarin KGM/HPMC, karuwar yawan juzu'i na tsarin zai haifar da haɗuwa da sauran hulɗar tsakanin sassan kwayoyin HPMC da KGM a cikin maganin, wanda zai rage motsi na sassan kwayoyin halitta, ta haka ne rage girman n darajar. tsarin. A lokaci guda, tare da karuwar abun ciki na KGM, hulɗar tsakanin sassan kwayoyin KGM a cikin tsarin KGM/HPMC yana inganta, ta haka ne ya rage motsinsa kuma yana haifar da raguwa a darajar n. Sabanin haka, ƙimar K na KGM/HPMC mahadi yana ƙaruwa tare da haɓakar abubuwan da ake buƙata da kuma abubuwan da ke cikin KGM, wanda galibi saboda haɓakar tsarin tsarin tsarin da abun ciki na KGM, waɗanda duka ke haɓaka abun ciki na kungiyoyin hydrophilic a cikin tsarin. , Ƙarfafa hulɗar kwayoyin halitta a cikin sassan kwayoyin halitta da kuma tsakanin sarƙoƙi, ta haka ne ya kara yawan radius na hydrodynamic na kwayoyin halitta, yana sa ya zama ƙasa da yiwuwar daidaitawa a ƙarƙashin aikin ƙarfin karfi na waje da kuma ƙara danko.

Za'a iya ƙididdige ƙimar ƙimar sifili-shear danko na tsarin fili na KGM/HPMC bisa ga ƙa'idar taƙaitaccen logarithmic da ke sama, kuma ana iya samun ƙimar gwajin sa ta Carren fitting extrapolation na madaidaicin ƙimar juzu'i. Idan aka kwatanta ƙimar da aka annabta na dankon sifili na tsarin fili na KGM/HPMC tare da nau'i-nau'i daban-daban da nau'o'in nau'i daban-daban tare da ƙimar gwaji, ana iya ganin ainihin ƙimar sifili-shear danko na KGM/HPMC fili. Magani ya fi ƙanƙanta darajar ka'idar. Wannan ya nuna cewa an kafa sabon taro tare da tsari mai yawa a cikin hadadden tsarin KGM da HPMC. Binciken da aka yi ya nuna cewa sassan mannose da ba a maye gurbinsu ba a kan sarkar kwayoyin halitta na KGM na iya yin hulɗa tare da ƙungiyoyin hydrophobic a kan sarkar kwayoyin halitta na HPMC don samar da yankin haɗin gwiwar hydrophobic mai rauni. Ana hasashe cewa sabon tsarin taro mai ƙanƙara tsari an samo shi ne ta hanyar hulɗar hydrophobic. Lokacin da rabon KGM ya yi ƙasa (HPMC> 50%), ainihin ƙimar sifili-shear danko na tsarin KGM/HPMC ya yi ƙasa da ƙimar ka'idar, wanda ke nuna cewa a ƙananan abun ciki na KGM, ƙarin ƙwayoyin suna shiga cikin sabon sabo. tsari. A cikin samuwar , an ƙara rage dankon sifili na tsarin.

2.2 Nazari na ƙwanƙwasa ɓarna na tsarin fili na KGM/HPMC

Daga ma'aunin dangantaka na modulus da nau'in nau'i na maganin KGM/HPMC tare da ɓangarorin taro daban-daban da nau'o'in haɗin kai daban-daban, ana iya ganin cewa lokacin da nau'in shear ya kasance ƙasa da 10%, G."kuma G"na fili tsarin m kada ka ƙara tare da karfi iri. Duk da haka, yana nuna cewa a cikin wannan nau'i na nau'i mai nau'i, tsarin haɗin gwiwar zai iya amsawa ga abubuwan da suka faru na waje ta hanyar sauya tsarin sarkar kwayoyin halitta, kuma tsarin tsarin fili bai lalace ba. Lokacin da nau'in shear ya kasance> 10%, na waje A ƙarƙashin aikin ƙarfin ƙarfi, saurin rarrabuwa na sarƙoƙi na ƙwayoyin cuta a cikin tsarin hadaddun ya fi saurin haɗuwa, G"kuma G"fara raguwa, kuma tsarin ya shiga yankin viscoelastic mara kyau. Sabili da haka, a cikin gwajin mitar mai ƙarfi na gaba, an zaɓi ma'aunin juzu'i azaman 1% don gwaji.

2.3 Matsakaicin la'akari da lankwasa na tsarin KGM/HPMC

Bambance-bambancen ma'auni na ma'auni da ma'aunin asara tare da mitar don mafita na KGM/HPMC tare da ma'auni daban-daban na haɓakawa a ƙarƙashin ɓangarori daban-daban. Ma'ajiyar modul G' tana wakiltar kuzarin da za'a iya dawo dasu bayan ajiya na wucin gadi a cikin gwajin, kuma ma'aunin asarar G' yana nufin makamashin da ake buƙata don kwararar farko, wanda shine asarar da ba za a iya jurewa ba kuma a ƙarshe an canza shi zuwa zafi mai ƙarfi. Ana iya ganin cewa, tare da Yayin da mitar oscillation ke ƙaruwa, ƙarancin ƙarancin G"koyaushe yana girma fiye da ma'ajin ajiya G", nuna halin ruwa. A cikin kewayon mitar gwaji, ma'aunin ma'ajin G' da asara modules G” suna ƙaruwa tare da haɓaka mitar oscillation. Wannan shi ne yafi saboda gaskiyar cewa tare da karuwa na mitar oscillation, sassan sassan kwayoyin halitta a cikin tsarin ba su da lokaci don dawowa zuwa nakasar a cikin ɗan gajeren lokaci Jihar da ta gabata, don haka yana nuna alamar cewa za a iya adana karin makamashi (mafi girma). babba G") ko yana buƙatar bata (G").

Tare da haɓakar mitar oscillation, tsarin ajiya na tsarin yana faduwa ba zato ba tsammani, kuma tare da haɓakar juzu'i mai yawa da abun ciki na KGM na tsarin, ƙimar mitar raguwa kwatsam a hankali yana ƙaruwa. Digowar kwatsam na iya zama saboda lalata ƙaƙƙarfan tsarin da aka kafa ta ƙungiyar hydrophobic tsakanin KGM da HPMC a cikin tsarin ta hanyar shear waje. Bugu da ƙari, haɓaka tsarin tsarin tsarin da abun ciki na KGM yana da amfani don kula da kwanciyar hankali na tsari mai yawa, kuma yana ƙara ƙimar mita na waje wanda ke lalata tsarin.

2.4 Zazzabi bincike mai lankwasa na KGM/HPMC

Daga madaidaicin ma'auni na ma'auni da ƙarancin asarar KGM/HPMC mafita tare da nau'i-nau'i daban-daban da ma'auni daban-daban, ana iya ganin cewa lokacin da yawancin tsarin ya kasance 0.50%, G."kuma G"na maganin HPMC da wuya ya canza tare da zafin jiki. , da G"> G", danko na tsarin ya mamaye; lokacin da yawan juzu'in ya karu, G"na maganin HPMC da farko ya kasance baya canzawa sannan ya karu sosai, kuma G"kuma G"shiga tsakani a wajen 70°C (Ma'aunin zafin jiki shine ma'anar gel), kuma tsarin yana samar da gel a wannan lokacin, don haka yana nuna cewa HPMC shine gel mai zafi. Don maganin KGM, lokacin da yawan adadin tsarin shine 0.50% da 0.75%, G"da G na tsarin “yana nuna raguwar yanayin; lokacin da yawan juzu'i ya karu, G' da G" na maganin KGM sun fara raguwa sannan kuma suna karuwa sosai, wanda ke nuna cewa maganin KGM yana nuna kaddarorin gel-kamar a manyan juzu'i da yanayin zafi.

Tare da karuwar zafin jiki, G"kuma G"na tsarin hadadden tsarin KGM/HPMC ya fara raguwa sannan ya karu sosai, kuma G"kuma G"ya bayyana wuraren haɗin gwiwa, kuma tsarin ya kafa gel. Lokacin da kwayoyin HPMC ke cikin ƙananan zafin jiki, haɗin gwiwar hydrogen yana faruwa tsakanin ƙungiyoyin hydrophilic akan sarkar kwayoyin halitta da kwayoyin ruwa, kuma lokacin da zafin jiki ya tashi, zafin da ake amfani da shi yana lalata haɗin haɗin hydrogen da aka samu tsakanin HPMC da kwayoyin ruwa, wanda ya haifar da samuwar HPMC macromolecular. sarƙoƙi. Ƙungiyoyin hydrophobic a saman suna fallasa, ƙungiyar hydrophobic yana faruwa, kuma an kafa gel na thermotropic. Don ƙananan tsarin juzu'i, ƙarin abubuwan KGM na iya samar da gel; don babban tsarin juzu'i, ƙarin abun ciki na HPMC na iya samar da gel. A cikin ƙananan tsarin juzu'i (0.50%), kasancewar ƙwayoyin KGM yana rage yiwuwar samar da haɗin gwiwar hydrogen tsakanin kwayoyin HPMC, don haka yana ƙara yiwuwar bayyanar da ƙungiyoyin hydrophobic a cikin kwayoyin HPMC, wanda ya dace da samuwar gels na thermotropic. A cikin babban tsarin juzu'i mai girma, idan abun ciki na KGM ya yi yawa, dankon tsarin yana da girma, wanda ba shi da amfani ga ƙungiyar hydrophobic tsakanin kwayoyin HPMC da KGM, wanda ba shi da kyau ga samuwar gel na thermogenic.

3. Kammalawa

A cikin wannan takarda, ana nazarin halayen rheological na tsarin fili na KGM da HPMC. Sakamakon ya nuna cewa tsarin fili na KGM/HPMC wani ruwa ne wanda ba na Newton ba ne, kuma nau'in ruwa na tsarin fili na KGM/HPMC an ƙaddara shi ne ta KGM. Ƙara yawan juzu'i na tsarin da abun ciki na KGM duka sun rage yawan ruwa na maganin fili kuma ya ƙara danko. A cikin jihar sol, sassan kwayoyin halitta na KGM da HPMC suna samar da tsari mai yawa ta hanyar hulɗar hydrophobic. Tsarin da ke cikin tsarin ya lalace ta hanyar tsagewar waje, yana haifar da raguwa kwatsam a cikin tsarin ajiya na tsarin. Ƙara yawan juzu'in tsarin tsarin da abun ciki na KGM yana da amfani don kula da kwanciyar hankali na tsari mai yawa da kuma ƙara ƙimar mitar waje wanda ke lalata tsarin. Don ƙananan tsarin juzu'i, ƙarin abun ciki na KGM yana dacewa da samuwar gel; don babban tsarin juzu'i mai yawa, ƙarin abun ciki na HPMC yana dacewa da samuwar gel.