Shiri na Hydrogel Microspheres daga Hydroxypropyl Methyl Cellulose

Wannan gwajin yana ɗaukar hanyar juzu'in dakatarwar lokaci ta polymerization, ta amfani da hydroxypropyl methylcellulose (HPMC) azaman albarkatun ƙasa, maganin sodium hydroxide azaman lokacin ruwa, cyclohexane azaman lokacin mai, da divinyl sulfone (DVS) azaman haɗin haɗin haɗin Tween- 20 da Span-60 a matsayin mai rarrabawa, yana motsawa a gudun 400-900r / min don shirya microspheres hydrogel.

Mabuɗin kalmomi: hydroxypropyl methylcellulose; hydrogel; microspheres; watsawa

1.Dubawa

1.1 Ma'anar hydrogel

Hydrogel (Hydrogel) wani nau'in nau'in nau'in polymer ne mai girma wanda ya ƙunshi ruwa mai yawa a cikin tsarin cibiyar sadarwa kuma ba ya narkewa a cikin ruwa. An gabatar da wani ɓangare na ƙungiyoyin hydrophobic da ragowar hydrophilic a cikin polymer mai narkewa mai ruwa tare da tsarin haɗin gwiwar cibiyar sadarwa, da kuma hydrophilic Ragowar suna ɗaure ga kwayoyin ruwa, suna haɗa kwayoyin ruwa a cikin hanyar sadarwa, yayin da ragowar hydrophobic suna kumbura da ruwa don samar da giciye. - hade da polymers. Jellies da ruwan tabarau a rayuwar yau da kullun duk samfuran hydrogel ne. Dangane da girman da siffar hydrogel, ana iya raba shi zuwa macroscopic gel da microscopic gel (microsphere), kuma tsohon za a iya raba shi zuwa columnar, soso mai laushi, fibrous, membranous, mai siffar zobe, da dai sauransu A halin yanzu da aka shirya microspheres da nanoscale microspheres. suna da laushi mai kyau, elasticity, ƙarfin ajiya na ruwa da kuma daidaitawa, kuma ana amfani da su a cikin binciken magungunan da aka kama.

1.2 Muhimmancin zaɓin jigo

A cikin 'yan shekarun nan, don biyan buƙatun kariyar muhalli, kayan aikin polymer hydrogel sun jawo hankalin jama'a a hankali saboda kyawawan kaddarorin hydrophilic da biocompatibility. An shirya microspheres na Hydrogel daga hydroxypropyl methylcellulose azaman albarkatun ƙasa a cikin wannan gwaji. Hydroxypropyl methylcellulose shine ether cellulose maras ionic, farin foda, maras wari kuma maras daɗi, kuma yana da halayen da ba za a iya maye gurbinsu da sauran kayan polymer na roba ba, don haka yana da ƙimar bincike mai girma a cikin filin polymer.

1.3 Matsayin ci gaba a gida da waje

Hydrogel wani nau'i ne na maganin magunguna wanda ya ja hankalin jama'ar likitocin duniya a cikin 'yan shekarun nan kuma ya bunkasa cikin sauri. Tun lokacin da Wichterle da Lim suka buga aikinsu na farko a kan HEMA masu haɗin gwiwar hydrogels a cikin 1960, bincike da bincike na hydrogels sun ci gaba da zurfafawa. A tsakiyar shekarun 1970s, Tanaka ya gano hydrogels masu raɗaɗi na pH lokacin da aka auna yawan kumburin gels na acrylamide, wanda ke nuna sabon mataki a cikin nazarin hydrogels. kasata tana cikin matakin ci gaban hydrogel. Saboda yawan shirye-shiryen magungunan gargajiya na kasar Sin da hadaddun sassa, yana da wahala a fitar da samfur guda daya mai tsafta yayin da abubuwa da yawa suka yi aiki tare, kuma adadin ya yi yawa, don haka ci gaban maganin hydrogel na kasar Sin na iya yin tafiyar hawainiya.

1.4 Kayan gwaji da ka'idoji

1.4.1 Hydroxypropyl methylcellulose

Hydroxypropyl methyl cellulose (HPMC), wanda ya samo asali ne daga methyl cellulose, wani muhimmin ether mai gauraye ne, wanda nasa ne na polymers masu narkewar ruwa marasa ionic, kuma ba shi da wari, mara daɗi kuma mara guba.

Masana'antu HPMC ne a cikin nau'i na fari foda ko fari sako-sako da fiber, da ruwa mai ruwa bayani yana da surface aiki, high nuna gaskiya da kuma barga yi. Saboda HPMC yana da mallaki na thermal gelation, samfurin ruwa mai ruwa bayani ne mai tsanani ga samar da wani gel da precipitates, sa'an nan narkar da bayan sanyaya, da gelation zafin jiki na daban-daban bayani dalla-dalla na samfurin ya bambanta. Kaddarorin dalla-dalla daban-daban na HPMC suma sun bambanta. Solubility yana canzawa tare da danko kuma ƙimar pH bai shafe shi ba. Ƙananan danko, mafi girma da solubility. Yayin da abun ciki na ƙungiyar metoxyl ya ragu, ma'anar gel na HPMC yana ƙaruwa, raguwar ruwa yana raguwa, kuma aikin saman yana raguwa. A cikin masana'antar biomedical, ana amfani da shi galibi azaman kayan sarrafa kayan polymer don kayan shafa, kayan fim, da shirye-shiryen sakewa mai dorewa. Hakanan za'a iya amfani dashi azaman stabilizer, wakili mai dakatarwa, mannen kwamfutar hannu, da mai haɓaka danko.

1.4.2 Ka'ida

Yin amfani da hanyar dawo da dakatarwar lokaci ta hanyar polymerization, ta amfani da Tween-20, Span-60 fili mai rarrabawa da Tween-20 azaman masu rarrabawa daban, ƙayyade ƙimar HLB (surfactant shine amphiphile tare da rukunin hydrophilic da ƙungiyar lipophilic Molecule, adadin girman da ƙarfi. ma'auni tsakanin ƙungiyar hydrophilic da ƙungiyar lipophilic a cikin ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta ta Cyclohexane na Cyclohexane na iya yin amfani da zafi mai zafi A cikin gwajin ci gaba da sashi shine sau 1-5 na maganin ruwa na monomer tare da maida hankali na 99% divinyl sulfone a matsayin wakili mai haɗawa, kuma ana sarrafa adadin ma'aunin haɗin gwiwa a kusan 10% na. busasshen cellulose taro, ta yadda mahara linzamin kwamfuta kwayoyin suna bonded da juna da kuma giciye-linked zuwa cibiyar sadarwa tsarin.

Ƙarfafawa yana da mahimmanci ga wannan gwaji, kuma ana sarrafa saurin gabaɗaya a kayan aiki na uku ko na huɗu. Domin girman saurin jujjuyawar kai tsaye yana shafar girman microspheres. Lokacin da saurin juyawa ya fi 980r / min, za a sami mummunan abin da ke danne bango, wanda zai rage yawan amfanin ƙasa; Wakilin haɗin giciye yana kula da samar da gels masu yawa, kuma ba za a iya samun samfurori masu siffar zobe ba.

2. Na'urorin gwaji da hanyoyin

2.1 Kayan Gwaji

Electronic balance, multifunctional lantarki stirrer, polarizing microscope, Malvern barbashi size analyzer.

Don shirya microspheres cellulose hydrogel, manyan sinadarai da ake amfani da su sune cyclohexane, Tween-20, Span-60, hydroxypropyl methylcellulose, divinyl sulfone, sodium hydroxide, ruwa mai narkewa, duk abin da ake amfani da monomers da additives kai tsaye ba tare da magani ba.

2.2 Matakan shiri na cellulose hydrogel microspheres

2.2.1 Amfani da Tween 20 azaman rarrabawa

Rushewar hydroxypropylmethylcellulose. Daidai auna 2g na sodium hydroxide kuma shirya maganin 2% sodium hydroxide tare da flask volumetric 100ml. Ɗauki 80ml na maganin sodium hydroxide da aka shirya da kuma dumama shi a cikin wanka na ruwa zuwa kusan 50°C, auna 0.2g na cellulose kuma ƙara shi a cikin maganin alkaline, motsa shi da sandar gilashi, sanya shi a cikin ruwan sanyi don wanka na kankara, sannan a yi amfani da shi azaman lokacin ruwa bayan an bayyana bayani. Yi amfani da silinda da aka kammala don auna 120ml na cyclohexane (lokacin mai) a cikin kwalba mai wuya uku, zana 5ml na Tween-20 a cikin man fetur tare da sirinji, kuma motsawa a 700r / min na sa'a daya. A samu rabin ruwan da aka shirya a zuba a cikin filasta mai wuya uku a yi ta motsawa na tsawon sa'o'i uku. Matsakaicin divinyl sulfone shine 99%, an diluted zuwa 1% da ruwa mai narkewa. Yi amfani da pipette don ɗaukar 0.5ml na DVS a cikin faifan volumetric 50ml don shirya 1% DVS, 1ml na DVS daidai yake da 0.01g. Yi amfani da pipette don ɗaukar 1ml a cikin kwandon wuyansa uku. Dama a dakin da zafin jiki na tsawon sa'o'i 22.

2.2.2 Amfani da span60 da Tween-20 azaman masu rarrabawa

Sauran rabin lokaci na ruwa da aka shirya yanzu. Auna nauyin 0.01gspan60 sannan a zuba a cikin bututun gwajin, sai a yi zafi a cikin ruwan wanka mai digiri 65 har sai ya narke, sai a sauke digo kadan na cyclohexane a cikin wankan ruwa tare da digon roba, sai a yi zafi har sai maganin ya zama fari fari. Ƙara shi a cikin kwalban wuyansa uku, sa'an nan kuma ƙara 120ml na cyclohexane, wanke bututun gwaji tare da cyclohexane sau da yawa, zafi don 5min, kwantar da hankali zuwa zafin jiki, kuma ƙara 0.5ml na Tween-20. Bayan yin motsawa na tsawon sa'o'i uku, an ƙara 1ml na diluted DVS. Dama a dakin da zafin jiki na tsawon sa'o'i 22.

2.2.3 Sakamakon gwaji

Samfurin da aka zuga an tsoma shi a cikin sandar gilashi kuma an narkar da shi a cikin 50ml na cikakken ethanol, kuma an auna girman barbashi a ƙarƙashin madaidaicin ƙwayar ƙwayar cuta na Malvern. Yin amfani da Tween-20 a matsayin microemulsion mai watsawa yana da kauri, kuma girman ƙwayar da aka auna na 87.1% shine 455.2d.nm, kuma girman barbashi na 12.9% shine 5026d.nm. The microemulsion na Tween-20 da Span-60 garwayayye dispersant ne kama da na madara, tare da 81.7% barbashi girman 5421d.nm da 18.3% barbashi girman 180.1d.nm.

3. Tattaunawar sakamakon gwaji

Don emulsifier don shirya microemulsion mai juyayi, sau da yawa yana da kyau a yi amfani da fili na surfactant hydrophilic da lipophilic surfactant. Wannan shi ne saboda solubility na surfactant guda ɗaya a cikin tsarin yana da ƙasa. Bayan an haɗa su biyun, ƙungiyoyin hydrophilic na Junansu da ƙungiyoyin lipophilic suna haɗin gwiwa tare da juna don samun sakamako mai narkewa. Ƙimar HLB kuma fihirisar da aka saba amfani da ita yayin zabar emulsifiers. Ta hanyar daidaita ƙimar HLB, za'a iya inganta rabon emulsifier mai sassa biyu, kuma ana iya shirya ƙarin microspheres iri ɗaya. A cikin wannan gwaji, an yi amfani da Span-60 mai rauni (HLB=4.7) da hydrophilic Tween-20 (HLB=16.7) azaman mai rarrabawa, kuma Span-20 an yi amfani da shi kaɗai azaman mai rarrabawa. Daga sakamakon gwaji, ana iya ganin cewa fili Tasirin ya fi mai rarraba guda ɗaya. Microemulsion na rarrabuwa na fili yana da ƙarancin daidaituwa kuma yana da daidaituwa kamar madara; da microemulsion ta amfani da guda dispersant yana da ma high danko da fari barbashi. Karamin kololuwar yana bayyana a ƙarƙashin mahaɗar fili na Tween-20 da Span-60. Dalili mai yiwuwa shi ne cewa tashin hankali na tsaka-tsaki na tsarin fili na Span-60 da Tween-20 yana da girma, kuma mai rarraba kanta ya rushe a ƙarƙashin babban ƙarfin motsawa don samar da ƙananan ƙwayoyin cuta za su shafi sakamakon gwaji. Rashin lahani na Tween-20 mai watsawa shine yana da adadi mai yawa na sarƙoƙi na polyoxyethylene (n = 20 ko makamancin haka), wanda ke sa tsangwama tsakanin ƙwayoyin surfactant ya fi girma kuma yana da wahala ya zama mai yawa a wurin dubawa. Yin la'akari da haɗuwa da zane-zane na girman barbashi, farin barbashi na iya zama cellulose wanda ba a tarwatsa ba. Sabili da haka, sakamakon wannan gwajin ya nuna cewa tasirin amfani da kayan tarwatsawa ya fi kyau, kuma gwajin zai iya kara rage yawan Tween-20 don sanya microspheres da aka shirya su zama daidai.

Bugu da ƙari, wasu kurakurai a cikin tsarin aikin gwaji ya kamata a rage su, kamar shirye-shiryen sodium hydroxide a cikin tsarin narkar da HPMC, dilution na DVS, da dai sauransu, ya kamata a daidaita daidai da yadda zai yiwu don rage kuskuren gwaji. Abu mafi mahimmanci shine adadin masu rarrabawa, saurin gudu da ƙarfin motsawa, da adadin ma'auni na haɗin giciye. Sai kawai lokacin da aka sarrafa da kyau za a iya shirya microspheres na hydrogel tare da watsawa mai kyau da girman nau'in nau'in nau'in.