Uphononongo ngokuziphatha kwe-rheological ye-konjac glucomannan kunye ne-hydroxypropyl methylcellulose system compound

Inkqubo ehlanganisiweyo ye-konjac glucomannan (KGM) kunye ne-hydroxypropyl methylcellulose (HPMC) yathathwa njengento yophando, kunye ne-steady-state shear, i-frequency kunye novavanyo lokutshayela lweqondo lokushisa lwenziwa kwi-compound system nge-rheometer ejikelezayo. Impembelelo yobuninzi besisombululo kunye ne-compound ratio kwi-viscosity kunye neempawu ze-rheological ze-KGM / HPMC inkqubo yekhompawundi yahlalutywa. Iziphumo zibonisa ukuba i-KGM / HPMC i-compound system i-non-Newtonian fluid, kunye nokunyuka kweqhezu lobuninzi kunye nomxholo we-KGM wenkqubo kunciphisa umbane wesisombululo kunye nokwandisa i-viscosity. Kwi-sol state, i-KGM kunye ne-HPMC ye-molecular chain zenza isakhiwo esincinci ngakumbi ngokusebenzisa i-hydrophobic interactions. Ukwandisa iqhezu lobunzima benkqubo kunye nomxholo we-KGM kulungele ukugcina uzinzo lwesakhiwo. Kwinkqubo yeqhekeza le-mass mass, ukwandisa umxholo we-KGM kunenzuzo ekubunjweni kweegels ze-thermotropic; ngelixa kwinkqubo yamaqhezu aphezulu, ukwandisa umxholo we-HPMC kukulungele ukusekwa kweegels ze-thermotropic.

Amagama angundoqo:ikonjac glucomannan; i-hydroxypropyl methylcellulose; ikhompawundi; ukuziphatha kwerheological

I-polysaccharides yendalo isetyenziswa ngokubanzi kwishishini lokutya ngenxa yokuqina, i-emulsifying kunye ne-gelling properties. I-Konjac glucomannan (KGM) sisityalo sendalo se-polysaccharide, esakhiweβ-D-glucose kunyeβ-D-mannose kumlinganiselo we-1.6: 1, ezi zimbini zidibaniswe yiβ-1,4 iibhondi ze-glycosidic, kwi-C- Kukho inani elincinci le-acetyl kwindawo ye-6 (malunga ne-acetyl ye-1 kuzo zonke ii-residues ze-17). Nangona kunjalo, i-viscosity ephezulu kunye ne-fluidity embi ye-KGM yesisombululo samanzi siyanciphisa ukusetyenziswa kwayo kwimveliso. IHydroxypropyl methylcellulose (HPMC) yipropylene glycol ether yemethylcellulose, eyeye-non-ionic cellulose ether. I-HPMC yenza ifilimu, inyibilika emanzini, kwaye iyahlaziyeka. I-HPMC ine-viscosity ephantsi kunye namandla ejeli kumaqondo obushushu aphantsi, kunye nokusebenza kakubi kokucubungula, kodwa inokwenza ijeli eqinileyo efana ne-viscous kumaqondo obushushu aphezulu, ngoko ke iinkqubo ezininzi zokuvelisa kufuneka zenziwe kumaqondo aphezulu, okukhokelela ekusetyenzisweni kwamandla aphezulu kwimveliso. Iindleko zemveliso ziphezulu. Uncwadi lubonisa ukuba iyunithi ye-mannose engatshintshiyo kwikhonkco ye-molecular ye-KGM ingenza ummandla obuthathaka odibeneyo we-hydrophobic association kunye neqela le-hydrophobic kwi-HPMC ye-molecular chain ngokusebenzisa i-hydrophobic interaction. Esi sakhiwo sinokulibazisa kwaye sithintele ngokuyinxenye i-thermal gelation ye-HPMC kunye nokunciphisa ubushushu bejel ye-HPMC. Ukongeza, ngokujonga iipropathi ezisezantsi ze-viscosity ze-HPMC kumaqondo obushushu aphantsi, kuqikelelwa ukuba ukudityaniswa kwayo ne-KGM kunokuphucula iipropathi ze-viscosity eziphezulu ze-KGM kunye nokuphucula ukusebenza kwayo. Ngoko ke, eli phepha liza kwakha inkqubo edibeneyo ye-KGM / HPMC ukuphonononga impembelelo ye-compound mass fraction kunye ne-compound ratio kwiipropati ze-rheological zenkqubo ye-KGM / HPMC, kwaye inike ireferensi yethiyori yokusetyenziswa kwenkqubo ye-KGM / HPMC ishishini lokutya.

1. Izinto kunye neendlela

1.1 Izixhobo kunye nee-reagents

IHydroxypropyl methylcellulose, KIMA CHEMICAL CO.,LTD, iqhezu lobunzima 2%, viscosity 6 mPa·s iqhezu le-methoxy mass 28% ~ 30%; i-hydroxypropyl mass fraction 7.0% ~ 12%.

Konjac glucomannan, Wuhan Johnson Konjac Food Co., Ltd., 1 wt% i-viscosity yesisombululo samanzi≥28 000 mPa·s.

1.2 Izixhobo kunye nezixhobo

I-MCR92 i-rheometer ejikelezayo, i-Anton Paar Co., Ltd., e-Austria; UPT-II-10T umatshini wamanzi we-ultrapure, iSichuan Youpu Ultrapure Technology Co., Ltd.; I-AB-50 ye-electronic analytical balance, inkampani yaseSwitzerland Mette; I-LHS-150HC yokuhlamba rhoqo ubushushu bamanzi, iWuxi Huaze Technology Co., Ltd.; JJ-1 Electric Stirrer, Jintan Medical Instrument Factory, kwiPhondo Jiangsu.

1.3 Ukulungiswa kwesisombululo esihlanganisiweyo

Yenza umlinganiselo we-HPMC kunye ne-KGM powders kunye nomlinganiselo othile wokudibanisa (i-mass ratio: 0:10, 3:7, 5:5, 7:3, 10:0), ngokucothayo wongeze emanzini adiyoni kwi-60.°C amanzi okuhlamba, kwaye ugxobhoze i-1.5 ~ 2 h ukuyenza ihlakazeke ngokulinganayo, kwaye ulungiselele iintlobo ezi-5 zezisombululo ze-gradient kunye namaqhezu obunzima obunzima be-0.50%, 0.75%, 1.00%, 1.25%, kunye ne-1.50% ngokulandelelana.

1.4 Uvavanyo lweepropati ze-rheological zesisombululo esixutywe

Uvavanyo lwe-shear oluzinzileyo: I-rheological curve ye-KGM / HPMC isisombululo sekhompawundi yalinganiswa ngokusebenzisa i-cone ye-CP50 kunye nepleyiti, i-gap phakathi kwamacwecwe angaphezulu nangaphantsi agxininiswe kwi-0.1 mm, ubushushu bokulinganisa be-25°C, kwaye umlinganiselo wokucheba wawuyi-0.1 ukuya kwi-100 s-1.

Ukuskena koxinzelelo (ukuzimisela kwengingqi ye-viscoelastic): Sebenzisa i-PP50 ipleyiti ukulinganisa ummandla we-viscoelastic kunye nomthetho wokutshintsha kwemodulus yesisombululo sekhompawundi ye-KGM / HPMC, setha isithuba kwi-1.000 mm, i-frequency esisigxina kwi-1Hz, kunye nobushushu bokulinganisa kwi-25°C. Uluhlu loxinzelelo yi-0.1% ~ 100%.

Ukutshayela rhoqo: Sebenzisa ipleyiti yePP50 ukulinganisa imodyuli yokutshintsha kunye nokuxhomekeka rhoqo kwesisombululo sekhompawundi ye-KGM/HPMC. Isithuba sibekwe kwi-1.000 mm, ubunzima yi-1%, ubushushu bomlinganiselo yi-25°C, kunye ne-frequency range yi-0.1-100 Hz.

Ukuskena kobushushu: I-modulus kunye nokuxhomekeka kwayo kwiqondo lokushisa kwe-KGM / HPMC isisombululo sekhompawundi yalinganiswa kusetyenziswa ipleyiti ye-PP50, isithuba sibekwe kwi-1.000 mm, i-frequency esisigxina yayiyi-1 Hz, i-deformation yayiyi-1%, kwaye ubushushu buvela kwi-25. ukuya ku90°C.

2. Iziphumo kunye noHlalutyo

2.1 Uhlalutyo lwe-curve ejikelezayo ye-KGM / HPMC inkqubo yekhompawundi

I-Viscosity ngokuchasene ne-chear rate curves ye-KGM/HPMC izisombululo ezinomlinganiselo wokudityaniswa ohlukeneyo kumaqhezu obunzima ohlukeneyo. Ulwelo oluthi i-viscosity ibe ngumsebenzi ongqameneyo wesantya sokucheba kuthiwa lulwelo lwe-Newtonian, kungenjalo kuthiwa lulwelo olungelulo olwase-Newtonian. Inokubonwa kwi-curve ukuba i-viscosity yesisombululo se-KGM kunye ne-KGM / HPMC isisombululo sekhompawundi siyancipha ngokunyuka kwesantya sokucheba; okukhona uphezulu umxholo we-KGM, kokukhona uphezulu inkqubo yobunzima beqhezu, kwaye kokukhona kucaca ngakumbi isenzeko sokucheba esibhityileyo sesisombululo. Oku kubonisa ukuba i-KGM kunye ne-KGM/HPMC i-compound system ayilulwelo olungelulo olwase-Newtonian, kwaye uhlobo lwe-fluid lwe-KGM/HPMC lwe-compound system lumiselwa ikakhulu yi-KGM.

Ukusuka kwisalathiso sokuhamba kunye ne-viscosity coefficient ye-KGM / HPMC izisombululo ezinamaqhezu ahlukeneyo obunzima kunye ne-compound ratios ezahlukeneyo, kunokubonwa ukuba ixabiso le-n ye-KGM, i-HPMC kunye ne-KGM / HPMC i-compound systems zonke zingaphantsi kwe-1, ebonisa ukuba izisombululo zonke ii-pseudoplastic fluids. Kwinkqubo ye-KGM / HPMC yekhompawundi, ukwanda kweqhezu lobunzima benkqubo kuya kubangela ukuxhamla kunye nokunye ukusebenzisana phakathi kwe-HPMC kunye ne-KGM ye-molecular chains kwisisombululo, esiya kunciphisa ukuhamba kwamakhonkco e-molecular, ngaloo ndlela ukunciphisa ixabiso le-n inkqubo. Ngexesha elifanayo, ngokunyuka komxholo we-KGM, intsebenziswano phakathi kwamaketanga e-molecular ye-KGM kwinkqubo ye-KGM / HPMC iphuculwe, ngaloo ndlela inciphisa ukuhamba kwayo kwaye ibangele ukuhla kwexabiso le-n. Ngokuchasene noko, ixabiso le-K lesisombululo se-KGM / HPMC liyanda ngokuqhubekayo kunye nokwanda kweqhezu lobuninzi besisombululo kunye nomxholo we-KGM, obangelwa kakhulu kukwanda kweqhekeza lenkqubo kunye nomxholo we-KGM, zombini ezonyusa umxholo we amaqela e-hydrophilic kwinkqubo. , ukunyusa intsebenziswano yeemolekyuli ngaphakathi kwekhonkco leemolekyuli kunye naphakathi kwamatyathanga, ngaloo ndlela inyusa i-radius ye-hydrodynamic ye-molecule, iyenza ukuba kungabi lula ukuqhelaniswa phantsi kwesenzo samandla e-shear yangaphandle kunye nokwandisa i-viscosity.

Ixabiso lethiyori ye-zero-shear viscosity ye-KGM/HPMC yekhompawundi yenkqubo ingabalwa ngokomgaqo-siseko we-logarithmic summation ongentla, kwaye ixabiso layo lokulinga linokufunyanwa ngeCarren fitting extrapolation ye-viscosity-shear rate curve. Ukuthelekisa ixabiso eliqikelelweyo le-viscosity ye-zero-shear ye-KGM/HPMC inkqubo edibeneyo kunye namaqhezu ahlukeneyo obunzima kunye nemilinganiselo eyahlukeneyo yokudibanisa kunye nexabiso lokulinga, kunokubonwa ukuba ixabiso lokwenene le-viscosity ye-zero-shear ye-KGM / HPMC ikhompawundi. isisombululo sincinci kunexabiso lethiyori. Oku kubonise ukuba indibano entsha enesakhiwo esixineneyo yasekwa kwinkqubo entsonkothileyo ye-KGM kunye ne-HPMC. Izifundo ezikhoyo zibonise ukuba iiyunithi ze-mannose ezingabandakanyiyo kwikhonkco le-molecular ye-KGM zinokusebenzisana namaqela e-hydrophobic kwi-HPMC ye-molecular chain ukwenza ummandla obuthathaka odibeneyo we-hydrophobic association. Kucingelwa ukuba isakhiwo esitsha sendibano esinesakhiwo esixineneyo senziwa ikakhulu ngokusebenzisana kwe-hydrophobic. Xa umlinganiselo we-KGM uphantsi (HPMC> 50%), ixabiso langempela le-zero-shear viscosity yenkqubo ye-KGM/HPMC iphantsi kunexabiso lethiyori, elibonisa ukuba kumxholo ophantsi we-KGM, iimolekyuli ezininzi zithatha inxaxheba kwi-denser entsha. isakhiwo. Ekuqulunqweni kwe-, i-viscosity ye-zero-shear yenkqubo iyancipha ngakumbi.

2.2 Uhlalutyo lwe-strain sweep curves ye-KGM/HPMC system compound

Ukusuka kwiigophe zobudlelwane bemodulus kunye noxinzelelo lwe-shear lwezisombululo ze-KGM/HPMC ezinamaqhezu ahlukeneyo obunzima kunye nemilinganiselo eyahlukeneyo edibeneyo, kunokubonwa ukuba xa ukucheba kungaphantsi kwe-10%, i-G"kunye noG″yenkqubo edityanisiweyo ngokwesiseko ayinyuki kunye noxinzelelo lokucheba. Nangona kunjalo, kubonisa ukuba ngaphakathi kolu luhlu lwe-shear strain, inkqubo ye-compound inokusabela kwi-stimuli yangaphandle ngokutshintshwa kwe-molecular chain conformation, kwaye isakhiwo senkqubo ye-compound ayonakaliswa. Xa i-shear strain i> 10%, yangaphandle Ngaphantsi kwesenzo samandla okucheba, isantya sokuqhawula amatyathanga eemolekyuli kwinkqubo entsonkothileyo sikhulu kunesantya sokungena, G"kunye noG″iqala ukuhla, kwaye inkqubo ingena kwindawo ye-viscoelastic engabonakaliyo. Ngoko ke, kuvavanyo olulandelayo lwe-frequency dynamic, iparameter ye-shear strain yakhethwa njenge-1% yokuvavanya.

2.3 Uhlalutyo lokutshayela rhoqo lwe-KGM / HPMC inkqubo edibeneyo

Iigophe eziguquguqukayo zemodyuli yokugcina kunye nemodyuli yokulahleka kunye ne-frequency ye-KGM / HPMC izisombululo ezinemilinganiselo eyahlukeneyo yokudibanisa phantsi kwamaqhezu ahlukeneyo obunzima. Imodyuli G' yogcino imele amandla anokubuyiselwa emva kokugcinwa okwethutyana kuvavanyo, kwaye imodyuli yokulahleka G” ithetha amandla afunekayo ekuqukuqeni kokuqala, eyilahleko engenakubuyiseka kwaye ekugqibeleni iguqulwe ibe bubushushu beshear. Inokubonwa ukuba, njengoko i-oscillation frequency isanda, ilahleko yemodyuli G″isoloko inkulu kunemodyuli yogcino G", ebonisa ukuziphatha kolwelo. Kuluhlu lwamaza ovavanyo, imodyuli yogcino G' kunye nemodyuli yelahleko G” yonyuka ngokunyuka kwe-oscillation frequency. Oku kubangelwa kakhulu kukuba ngokunyuka kwe-oscillation frequency, amacandelo e-molecular chain chain kwinkqubo ayinalo ixesha lokubuyisela kwi-deformation ngexesha elifutshane Imeko yangaphambili, ngaloo ndlela ibonisa into yokuba amandla amaninzi anokugcinwa ( mkhulu G") okanye kufuneka ilahleke (G″).

Ngokunyuka kwe-oscillation frequency, i-modulus yokugcina yenkqubo ihla ngokukhawuleza, kwaye ngokunyuka kweqhezu lobunzima kunye nomxholo we-KGM wenkqubo, i-frequency point of drops ngokukhawuleza iyanda. Ukuhla ngokukhawuleza kunokuba ngenxa yokutshatyalaliswa kwesakhiwo esidibeneyo esenziwe ngumbutho we-hydrophobic phakathi kwe-KGM kunye ne-HPMC kwinkqubo ngokucheba kwangaphandle. Ngaphezu koko, ukunyuka kwecandelo lobunzima benkqubo kunye nomxholo we-KGM kunenzuzo ukugcina uzinzo lwesakhiwo esixineneyo, kunye nokwandisa ixabiso lefrikhwensi yangaphandle etshabalalisa isakhiwo.

2.4 Uhlalutyo lokuskena lobushushu lwenkqubo ehlanganisiweyo ye-KGM/HPMC

Ukusuka kwiigophe zemodyuli yokugcina kunye ne-modulus yokulahleka kwe-KGM / HPMC izisombululo ezinamaqhezu ahlukeneyo obunzima kunye nemilinganiselo eyahlukeneyo yokudibanisa, kunokubonwa ukuba xa i-fraction mass yenkqubo i-0.50%, i-G"kunye noG″yesisombululo HPMC kunzima ukutshintsha ubushushu. kunye, kunye noG″> G", i-viscosity yenkqubo ilawula; xa iqhezu lobunzima linyuka, i-G"yesisombululo se-HPMC kuqala ihlala ingatshintshi kwaye inyuke kabukhali, kwaye i-G"kunye noG″ukunqumla malunga ne-70°C (Iqondo lokushisa le-intersection liyi-gel point), kwaye inkqubo yenza i-gel ngeli xesha, ngaloo ndlela ibonisa ukuba i-HPMC i-gel ye-thermal-induced. Kwisisombululo se-KGM, xa iqhezu lobunzima benkqubo yi-0.50% kunye ne-0.75%, i-G"kunye no-G wenkqubo “ubonisa indlela ehlayo; xa iqhezu lobuninzi linyuka, i-G' kunye ne-G yesisombululo se-KGM iyancipha kuqala kwaye inyuke kakhulu, nto leyo ebonisa ukuba isisombululo se-KGM sibonisa iimpawu ezifana nejeli kumaqhezu aphezulu kunye namaqondo obushushu aphezulu .

Ngokunyuka kwamaqondo obushushu, iG"kunye noG″yenkqubo entsonkothileyo ye-KGM/HPMC yaqala yehla yaza yanda kakhulu, kwaye i-G"kunye noG″kwavela iindawo zokuhlangana, kwaye inkqubo yenza ijeli. Xa iimolekyuli ze-HPMC zikwiqondo lobushushu eliphantsi, ukudityaniswa kwe-hydrogen kwenzeka phakathi kwamaqela e-hydrophilic kwitsheyina lemolekyuli kunye neemolekyuli zamanzi, kwaye xa ubushushu buphakama, ubushushu obusetyenzisiweyo butshabalalisa iibhondi ze-hydrogen ezenziwe phakathi kwe-HPMC kunye neemolekyuli zamanzi, okukhokelela ekusekweni kwe-HPMC macromolecular. imixokelelwane. Amaqela e-hydrophobic ebusweni abonakaliswe, i-hydrophobic association iyenzeka, kwaye i-gel ye-thermotropic yenziwa. Kwinkqubo yamaqhezu anobunzima obuphantsi, umxholo omninzi we-KGM unokwenza ijeli; kwinkqubo yeqhezu lobunzima obuphezulu, umxholo omninzi we-HPMC unokwenza ijeli. Kwinkqubo yeqhekeza ubunzima obuphantsi (0.50%), ubukho beemolekyuli ze-KGM kunciphisa amathuba okwenza iibhondi ze-hydrogen phakathi kwee-molecule ze-HPMC, ngaloo ndlela kwandisa amathuba okuvezwa kwamaqela e-hydrophobic kwii-molecule ze-HPMC, ezenza kube lula ukubunjwa kweejeli ze-thermotropic. Kwinkqubo ye-fraction ye-mass mass, ukuba umxholo we-KGM uphezulu kakhulu, i-viscosity yenkqubo iphezulu, engahambelani nombutho we-hydrophobic phakathi kwe-HPMC kunye ne-KGM molekyuli, engabandakanyi ukubunjwa kwe-gel thermogenic.

3. Isiphelo

Kweli phepha, ukuziphatha kwe-rheological yenkqubo edibeneyo ye-KGM kunye ne-HPMC ifundwa. Iziphumo zibonisa ukuba i-compound system ye-KGM/HPMC ayilulwelo olungelulo olwase-Newtonian, kwaye uhlobo lwe-fluid lwe-compound system ye-KGM/HPMC lumiselwa ikakhulu yi-KGM. Ukwandisa inkqubo yobuninzi beqhezu kunye nomxholo we-KGM zombini kunciphisa ubuninzi besisombululo esixutywe kunye nokwandisa i-viscosity yayo. Kwi-sol state, i-molecular chains ye-KGM kunye ne-HPMC yenza isakhiwo esixineneyo ngokusebenzisa i-hydrophobic interactions. Isakhiwo kwinkqubo kutshatyalaliswa ukucheba kwangaphandle, okubangela ukuhla ngokukhawuleza kwimodyuli yokugcina inkqubo. Ukonyuka kweqhezu le-mass mass kunye nomxholo we-KGM kunenzuzo ukugcina uzinzo lwesakhiwo esixineneyo kunye nokwandisa ixabiso lefrikhwensi yangaphandle etshabalalisa isakhiwo. Kwinkqubo yeqhezu lobunzima obuphantsi, umxholo omninzi we-KGM ulungele ukwenziwa kwejeli; kwinkqubo yeqhezu lobunzima obuphezulu, umxholo ongaphezulu we-HPMC ulungele ukwenziwa kwejeli.