Boithuto ka boits'oaro ba rheological ba konjac glucomannan le hydroxypropyl methylcellulose system

Sisteme ea komporo ea konjac glucomannan (KGM) le hydroxypropyl methylcellulose (HPMC) e nkiloe e le ntho ea lipatlisiso, 'me liteko tse tsitsitseng tsa ho kuta, maqhubu le mocheso li ile tsa etsoa tsamaisong ea motsoako ka rheometer e potolohang. Tšusumetso ea karolo ea boima ba tharollo le karo-karolelano ea motsoako ho viscosity le thepa ea rheological ea tsamaiso ea metsoako ea KGM/HPMC e ile ea hlahlojoa. Liphello li bontša hore tsamaiso ea lik'hemik'hale tsa KGM / HPMC ke mokelikeli o seng oa Newtonian, 'me ho eketseha ha karoloana ea boima le lihlahisoa tsa KGM tsa tsamaiso ho fokotsa metsi a motsoako oa motsoako le ho eketsa viscosity. Sebakeng sa sol, liketane tsa limolek'hule tsa KGM le HPMC li theha sebopeho se kopaneng haholoanyane ka litšebelisano tsa hydrophobic. Ho eketsa karolo ea boima ba tsamaiso le litaba tsa KGM li thusa ho boloka botsitso ba sebopeho. Tsamaisong e tlaase ea likaroloana tsa boima, ho eketsa litaba tsa KGM ho molemo ho thehoeng ha li-gel tsa thermotropic; ha e ntse e le tsamaisong e phahameng ea likaroloana, ho eketsa litaba tsa HPMC ho loketse ho thehoa ha li-gel tsa thermotropic.

Mantsoe a bohlokoa:konjac glucomannan; hydroxypropyl methylcellulose; motsoako; boitšoaro ba rheological

Li-polysaccharides tsa tlhaho li sebelisoa haholo indastering ea lijo ka lebaka la thepa ea tsona e teteaneng, e emulsifying le ea gelling. Konjac glucomannan (KGM) ke semela sa tlhaho sa polysaccharide, se entsoeng kaβ-D-glucose leβ-D-mannose ka karo-karolelano ea 1.6: 1, tse peli li kopantsoe keβ-1,4 glycosidic bonds, ho C- Ho na le acetyl e nyenyane sebakeng sa 6 (hoo e ka bang 1 acetyl bakeng sa masala a mang le a mang a 17). Leha ho le joalo, viscosity e phahameng le metsi a fokolang a tharollo ea metsi a KGM a fokotsa ts'ebeliso ea eona tlhahisong. Hydroxypropyl methylcellulose (HPMC) ke propylene glycol ether ea methylcellulose, eo e leng ea non-ionic cellulose ether. HPMC e etsa lifilimi, e qhibiliha ka metsing, ebile e ka nchafatsoa. HPMC e na le viscosity e tlase le matla a gel ka mocheso o tlase, le ts'ebetso e fokolang ea ts'ebetso, empa e ka etsa gel e batlang e le ea viscous solid-joalo ka mocheso o phahameng, kahoo mekhoa e mengata ea tlhahiso e tlameha ho etsoa ka mocheso o phahameng, e leng se bakang tšebeliso e phahameng ea matla a tlhahiso. Litšenyehelo tsa tlhahiso li holimo. Lingoliloeng li bontša hore sehlopha sa mannose se sa nkeng sebaka sa ketane ea molek'hule ea KGM se ka theha sebaka se fokolang sa hydrophobic association le sehlopha sa hydrophobic ka ketane ea molek'hule ea HPMC ka ho sebelisana ha hydrophobic. Sebopeho sena se ka lieha le ho thibela ka mokhoa o sa fellang mocheso oa mocheso oa HPMC le ho theola mocheso oa gel oa HPMC. Ho phaella moo, ka lebaka la thepa e tlaase ea viscosity ea HPMC ka mocheso o batlang o le tlaase, ho boleloa esale pele hore ho kopanya ha eona le KGM ho ka ntlafatsa thepa e phahameng ea viscosity ea KGM le ho ntlafatsa ts'ebetso ea eona ea ts'ebetso. Ka hona, pampiri ena e tla haha ​​​​motsoako oa KGM / HPMC ho hlahloba tšusumetso ea karoloana ea boima ba tharollo le karo-karolelano ea metsoako ho thepa ea rheological ea tsamaiso ea KGM / HPMC, 'me e fane ka boitsebiso ba khopolo-taba bakeng sa tšebeliso ea tsamaiso ea lik'hemik'hale tsa KGM / HPMC ho. indasteri ea lijo.

1. Lisebelisoa le mekhoa

1.1 Lisebelisoa le li-reagents

Hydroxypropyl methylcellulose, KIMA CHEMICAL CO.,LTD, karolo ea boima 2%, viscosity 6 mPa·s; karoloana ea boima ba methoxy 28% ~ 30%; karolo ea boima ba hydroxypropyl 7.0% ~ 12%.

Konjac glucomannan, Wuhan Johnson Konjac Food Co., Ltd., 1 wt% viscosity ea tharollo ea metsi≥28 000 mPa·s.

1.2 Lisebelisoa le lisebelisoa

MCR92 rotational rheometer, Anton Paar Co., Ltd., Austria; UPT-II-10T mochini oa metsi oa ultrapure, Sichuan Youpu Ultrapure Technology Co., Ltd.; AB-50 elektroniki analytical balance, Swiss Mette company; LHS-150HC ea ho hlapa metsi kamehla mocheso, Wuxi Huaze Technology Co., Ltd.; JJ-1 Electric Stirrer, Jintan Medical Instrument Factory, Profinseng ea Jiangsu.

1.3 Tokisetso ea motsoako oa motsoako

Beha phofo ea HPMC le KGM ka tekanyo e itseng ea motsoako (karolelano ea boima: 0:10, 3:7, 5:5, 7:3, 10:0), butle-butle u li kenye metsing a hloekisitsoeng ka 60.°C ho hlapa metsi, 'me u hlohlelletse bakeng sa 1.5 ~ 2 h ho etsa hore e qhalakanngoe ka mokhoa o ts'oanang,' me u lokise mefuta e 5 ea litharollo tsa gradient ka likaroloana tse tiileng tsa boima ba 0.50%, 0.75%, 1.00%, 1.25%, le 1.50%, ka ho latellana.

1.4 Teko ea thepa ea rheological ea motsoako oa motsoako

Teko e tsitsitseng ea ho kuta: Mokokotlo oa rheological oa tharollo ea motsoako oa KGM / HPMC o ile oa lekanyetsoa ho sebelisoa CP50 cone le poleiti, lekhalo pakeng tsa lipoleiti tse ka holimo le tse tlaase li ne li behiloe ho 0.1 mm, mocheso oa tekanyo e ne e le 25°C, 'me sekhahla sa sekhahla sa ho kuta e ne e le 0.1 ho isa ho 100 s-1.

Strain scanning (boikemisetso ba sebaka sa linear viscoelastic): Sebelisa poleiti ea PP50 ho lekanya sebaka se melang sa viscoelastic le modulus phetoho molao oa tharollo ea motsoako oa KGM/HPMC, beha sebaka ho 1.000 mm, frequency e tsitsitseng ho 1Hz, le mocheso oa tekanyo ho 25°C. Mofuta oa khatello ke 0.1% ~ 100%.

Ho fiela khafetsa: Sebelisa poleiti ea PP50 ho lekanya phetoho ea modulus le ho itšetleha ka makhetlo a tharollo ea motsoako oa KGM/HPMC. Sebaka se behiloe ho 1.000 mm, khatello ke 1%, mocheso oa tekanyo ke 25°C, 'me lebelo la maqhubu ke 0.1-100 Hz.

Ho hlahlojoa ha mocheso: Modulus le mocheso oa eona o itšetlehileng ka tharollo ea lik'hemik'hale tsa KGM / HPMC li ne li lekantsoe ka poleiti ea PP50, sebaka se behiloe ho 1.000 mm, maqhubu a tsitsitseng e ne e le 1 Hz, deformation e ne e le 1%, 'me mocheso o tsoa ho 25. ho 90°C.

2. Liphetho le Analysis

2.1 Tlhahlobo ea phallo ea phallo ea tsamaiso ea metsoako ea KGM / HPMC

Viscosity khahlano le shear rate curves ea tharollo ea KGM/HPMC e nang le likarohano tse fapaneng tsa motsoako likarolong tse fapaneng tsa boima. Mekelikeli eo viscosity ea eona e leng tšebetso e tšoanang ea sekhahla sa ho kuta e bitsoa maro a Newtonian, ho seng joalo a bitsoa maro a non-Newtonian. E ka bonoa ho tloha mokokotlong hore viscosity ea tharollo ea KGM le tharollo ea metsoako ea KGM / HPMC e fokotseha ka ho eketseha ha sekhahla sa ho kuta; ha litaba tsa KGM li phahame, karolo ea boima ba tsamaiso e phahame, 'me ho totobetse haholoanyane ho fokotsa sekhahla sa tharollo. Sena se bontša hore tsamaiso ea metsoako ea KGM le KGM/HPMC ha se maro a Newtonian, 'me mofuta oa mokelikeli oa tsamaiso ea motsoako oa KGM/HPMC o khethoa haholo ke KGM.

Ho tsoa ho index ea phallo le coefficient ea viscosity ea tharollo ea KGM / HPMC e nang le likaroloana tse fapaneng tsa boima le likarolo tse fapaneng tsa metsoako, ho ka bonoa hore litekanyetso tsa n tsa KGM, HPMC le KGM / HPMC litsamaiso tsa komporo kaofela li ka tlase ho 1, ho bonts'a hore tharollo ke maro a pseudoplastic. Bakeng sa tsamaiso ea lik'hemik'hale tsa KGM / HPMC, ho eketseha ha karolo ea bongata ba tsamaiso ho tla baka ho ts'oaroa le likamano tse ling pakeng tsa liketane tsa molek'hule tsa HPMC le KGM ka tharollo, e leng ho tla fokotsa ho tsamaea ha liketane tsa limolek'hule, ka hona ho fokotsa boleng ba n. tsamaiso. Ka nako e ts'oanang, ka keketseho ea litaba tsa KGM, tšebelisano pakeng tsa liketane tsa limolek'hule tsa KGM tsamaisong ea KGM / HPMC ea ntlafatsoa, ​​kahoo e fokotsa ho tsamaea ha eona le ho fella ka ho fokotseha ha boleng ba n. Ho fapana le hoo, boleng ba K ea tharollo ea kompone ea KGM/HPMC bo ntse bo eketseha ka ho eketseha ha karoloana ea boima ba tharollo le litaba tsa KGM, e leng haholo-holo ka lebaka la keketseho ea karoloana ea boima ba tsamaiso le litaba tsa KGM, tseo ka bobeli li eketsang litaba tsa lihlopha tsa hydrophilic tsamaisong. , ho eketsa tšebelisano ea limolek'hule ka har'a ketane ea limolek'hule le pakeng tsa liketane, ka tsela eo ho eketsa radius ea hydrodynamic ea molek'hule, ho etsa hore ho se ke ha e-ba bonolo ho sekamela tlas'a ketso ea matla a ho kuta ka ntle le ho eketsa viscosity.

Boleng ba theory ea viscosity ea zero-shear ea sistimi ea komporo ea KGM / HPMC e ka baloa ho latela molao-motheo o ka holimo oa logarithmic summation, mme boleng ba eona ba liteko bo ka fumanoa ka Carren e loketseng expolation ea viscosity-shear rate curve. Ha ho bapisoa boleng bo boletsoeng esale pele ba viscosity ea zero-shear ea tsamaiso ea lik'hemik'hale ea KGM / HPMC e nang le likaroloana tse fapaneng tsa boima le likaroloana tse fapaneng tse kopanyang le boleng ba liteko, ho ka bonoa hore boleng ba sebele ba viscosity ea zero-shear ea motsoako oa KGM / HPMC. tharollo e nyane ho feta boleng ba theory. Sena se bontšitse hore kopano e ncha e nang le mohaho o teteaneng e thehiloe tsamaisong e rarahaneng ea KGM le HPMC. Liphuputso tse seng li ntse li le teng li bontšitse hore lihlopha tsa mannose tse sa kenngoeng ketane ea limolek'hule tsa KGM li ka sebelisana le lihlopha tsa hydrophobic ka ketane ea molek'hule ea HPMC ho theha sebaka se fokolang sa hydrophobic association. Ho nahanoa hore mohaho o mocha oa kopano o nang le mohaho o batlang o teteane o thehoa haholo-holo ka ho sebelisana ha hydrophobic. Ha karo-karolelano ea KGM e le tlase (HPMC> 50%), boleng ba sebele ba zero-shear viscosity ea tsamaiso ea KGM/HPMC bo ka tlaase ho boleng ba khopolo-taba, e leng se bontšang hore ka litaba tse tlaase tsa KGM, limolek'hule tse ngata li kenya letsoho ho tse ncha tse teteaneng. sebopeho. Ha ho thehoa , viscosity ea zero-shear ea tsamaiso e fokotsehile le ho feta.

2.2 Tlhahlobo ea li-curve tsa khatello ea methapo ea tsamaiso ea KGM/HPMC

Ho tsoa ho li-curve tsa likamano tsa modulus le khatello ea ho kuta ea tharollo ea KGM/HPMC e nang le likaroloana tse fapaneng tsa boima le likarolo tse fapaneng tsa motsoako, ho ka bonoa hore ha khatello ea ho kuta e ka tlase ho 10%, G."le G″ea tsamaiso ea motsoako ha e le hantle u se ke ua eketseha ka khatello ea ho kuta. Leha ho le joalo, e bontša hore ka har'a mefuta ena ea khatello ea ho kuta, tsamaiso ea lik'hemik'hale e ka arabela ts'ebetsong ea ka ntle ka ho fetoha ha li-molecular chain conformation, 'me sebopeho sa tsamaiso ea metsoako ha se senyehe. Ha khatello ea ho kuta e le> 10%, e ka ntle Tlas'a ketso ea matla a ho kuta, lebelo la ho arohana ha liketane tsa limolek'hule tsamaisong e rarahaneng e kholo ho feta lebelo la ho kenngoa, G."le G″qala ho fokotseha, 'me tsamaiso e kena sebakeng se sa tloaelehang sa viscoelastic. Ka hona, tekong e latelang ea matla a maqhubu, parameter ea khatello ea shear e khethiloe e le 1% bakeng sa tlhahlobo.

2.3 Ts'ebetso ea khafetsa ea sweep curve ea tsamaiso ea metsoako ea KGM/HPMC

Phapang ea li-curve tsa modulus ea polokelo le modulus ea tahlehelo e nang le khafetsa bakeng sa tharollo ea KGM/HPMC e nang le likarohano tse fapaneng tse kopanyang tlasa likaroloana tse fapaneng tsa boima. Modulus ea polokelo ea G' e emela matla a ka fumanoang ka mor'a ho bolokoa ka nakoana tekong, 'me tahlehelo ea modulus G "e bolela matla a hlokahalang bakeng sa phallo ea pele, e leng tahlehelo e ke keng ea etsolloa 'me qetellong e fetoloa mocheso oa shear. Hoa bonahala hore, ha maqhubu a oscillation a ntse a eketseha, tahlehelo ea modulus G″e lula e le kholo ho feta modulus ea polokelo ea G", e bontšang boitšoaro ba mokelikeli. Boemong ba maqhubu a teko, modulus ea polokelo ea G' le modulus ea tahlehelo ea G” lia eketseha ka ho eketseha ha maqhubu a oscillation. Sena se bakoa haholo ke taba ea hore ka ho eketseha ha maqhubu a oscillation, likarolo tsa ketane ea limolek'hule tsamaisong ha li na nako ea ho hlaphoheloa ho deformation ka nako e khutšoanyane 'Muso o fetileng, kahoo o bontša ketsahalo ea hore matla a mangata a ka bolokoa. e kholoanyane G") kapa e hloka ho lahleha (G″).

Ka ho eketseha ha maqhubu a oscillation, modulus ea polokelo ea tsamaiso e theoha ka tšohanyetso, 'me ka ho eketseha ha karoloana ea boima le KGM ea tsamaiso, sekhahla sa ho theoha ka tšohanyetso se eketseha butle-butle. Ho theoha ha tšohanyetso ho ka 'na ha e-ba ka lebaka la ho senyeha ha mohaho o kopanetsoeng o entsoeng ke mokhatlo oa hydrophobic pakeng tsa KGM le HPMC tsamaisong ka ho kuta ka ntle. Ho feta moo, keketseho ea karoloana ea boima ba tsamaiso le litaba tsa KGM li molemo ho boloka botsitso ba sebopeho se teteaneng, le ho eketsa boleng ba maqhubu a kantle a senyang sebopeho.

2.4 Tlhahlobo ea curve ea ho hlahloba mocheso oa tsamaiso e kopanetsoeng ea KGM/HPMC

Ho tloha ho li-curve tsa modulus ea polokelo le tahlehelo ea modulus ea tharollo ea KGM / HPMC e nang le likaroloana tse fapaneng tsa boima le likarolo tse fapaneng tsa motsoako, ho ka bonoa hore ha karolo ea boima ba tsamaiso e le 0.50%, G."le G″tharollo ea HPMC ha e fetohe le mocheso. , le G″> G", viscosity ea tsamaiso e laola; ha karolo ea boima e eketseha, G"ea tharollo ea HPMC pele e lula e sa fetohe ebe e eketseha haholo, 'me G"le G″ho pota-pota hoo e ka bang 70°C (Mocheso oa ntlha ea mateano ke sebaka sa gel), 'me tsamaiso e etsa gel ka nako ena, kahoo e bontša hore HPMC ke gel e entsoeng ka mocheso. Bakeng sa tharollo ea KGM, ha karolo e boima ea tsamaiso e le 0.50% le 0.75%, G."le G ea tsamaiso “e bontša mokhoa o ntseng o fokotseha; ha karolo ea bongata e eketseha, G' le G” ea tharollo ea KGM e qala ho fokotseha ebe e eketseha haholo, e leng se bontšang hore tharollo ea KGM e bontša thepa e kang ea gel likarolong tse phahameng tsa boima le mocheso o phahameng .

Ka ho eketseha ha mocheso, G"le G″ea tsamaiso e rarahaneng ea KGM/HPMC e ile ea qala ho fokotseha 'me ea eketseha haholo, 'me G"le G″ho ile ha hlaha libaka tsa mateano, 'me tsamaiso ea theha gel. Ha limolek'hule tsa HPMC li le mocheso o tlaase, ho kopana ha hydrogen ho etsahala pakeng tsa lihlopha tsa hydrophilic holim'a ketane ea molek'hule le limolek'hule tsa metsi, 'me ha mocheso o phahama, mocheso o sebelisoang o senya likamano tsa hydrogen tse entsoeng pakeng tsa HPMC le limolek'hule tsa metsi, e leng se etsang hore ho thehoe HPMC macromolecular. diketane. Lihlopha tsa hydrophobic tse holim'a metsi li pepesitsoe, mokhatlo oa hydrophobic o etsahala, 'me ho thehoa gel ea thermotropic. Bakeng sa tsamaiso ea likaroloana tsa boima bo tlaase, litaba tse ngata tsa KGM li ka etsa gel; bakeng sa sistimi e phahameng ea likarolo, litaba tse ngata tsa HPMC li ka theha gel. Lenaneong le tlase la karolo ea boima (0.50%), ho ba teng ha limolek'hule tsa KGM ho fokotsa monyetla oa ho theha maqhama a haedrojene pakeng tsa limolek'hule tsa HPMC, ka hona ho eketsa monyetla oa ho pepeseha ha lihlopha tsa hydrophobic ka limolek'hule tsa HPMC, tse loketseng ho thehoa ha li-gel tsa thermotropic. Tsamaisong e phahameng ea likaroloana, haeba litaba tsa KGM li phahame haholo, viscosity ea tsamaiso e phahame, e sa lumellaneng le mokhatlo oa hydrophobic pakeng tsa HPMC le limolek'hule tsa KGM, tse sa lumellaneng le ho thehoa ha gel ea thermogenic.

3. Qetello

Ka pampiri ena, boitšoaro ba rheological ba tsamaiso ea metsoako ea KGM le HPMC bo ithutoa. Liphello li bontša hore tsamaiso ea metsoako ea KGM/HPMC ke mokelikeli oo e seng oa Newtonian, 'me mofuta oa mokelikeli oa tsamaiso ea motsoako oa KGM/HPMC o khethoa haholo ke KGM. Ho eketsa karolo ea boima ba tsamaiso le litaba tsa KGM ka bobeli li fokolitse metsi a tharollo ea motsoako le ho eketsa viscosity ea eona. Sebakeng sa Sol, liketane tsa limolek'hule tsa KGM le HPMC li theha sebopeho se teteaneng ka ho sebelisana ha hydrophobic. Sebopeho sa tsamaiso se senngoa ke ho kuta ka ntle, ho fella ka ho theoha ka tšohanyetso modulus ea polokelo ea tsamaiso. Keketseho ea karolo ea boima ba sistimi le litaba tsa KGM li molemong oa ho boloka botsitso ba sebopeho se teteaneng le ho eketsa boleng ba maqhubu a kantle a senyang sebopeho. Bakeng sa tsamaiso ea likaroloana tsa boima bo tlaase, litaba tse ngata tsa KGM li loketse ho thehoa ha gel; bakeng sa tsamaiso ea likarolo tse phahameng tsa boima, litaba tse ngata tsa HPMC li loketse ho thehoa ha gel.