Diyaarinta Hydrogel Microspheres ka Hydroxypropyl Methyl Cellulose

Tijaabadani waxay qabataa habka dib-u-hakinta wejiga polymerization, iyadoo la adeegsanayo hydroxypropyl methylcellulose (HPMC) sida walxaha ceeriin, xalka sodium hydroxide sida wajiga biyaha, cyclohexane oo ah wajiga saliidda, iyo divinyl sulfone (DVS) oo ah isku dhafka isku dhafka ah ee Tween- 20 iyo Span-60 sida kala firdhiso, walaaqaya xawaare 400-900r / min si loo diyaariyo microspheres hydrogel.

Erayada muhiimka ah: hydroxypropyl methylcellulose; hydrogel; microspheres; kala firdhiyey

1.Dulmar

1.1 Qeexida hydrogel

Hydrogel (Hydrogel) waa nooc ka mid ah polymer-ka molecular sare oo ka kooban xaddi badan oo biyo ah oo ku jira qaab dhismeedka shabakadda oo aan lagu milmi karin biyaha. Qayb ka mid ah kooxaha hydrophobic iyo hadhaaga hydrophilic ayaa lagu soo dhex geliyaa polymer-ka biyaha ku milma oo leh shabakad isku xiran oo isku xiran, iyo hydrophilic Haraaga wuxuu ku xiran yahay molecules biyaha, isku xirka molecules biyaha gudaha shabakada, halka haraaga hydrophobic ay bararaan biyo si ay u sameeyaan iskutallaab. polymers-ku xiran. Jeelliyada iyo muraayadaha indhaha ee nolol maalmeedka waa dhammaan alaabada hydrogel. Marka loo eego cabbirka iyo qaabka hydrogel, waxaa loo qaybin karaa jel makroscopic iyo jel microscopic (microsphere), kan horena waxaa loo qaybin karaa columnar, sponge dalool, fibrous, membranous, spherical, iwm. waxay leeyihiin jilicsanaan wanaagsan, dabacsanaan, awoodda kaydinta dareeraha iyo biocompatibility, waxaana loo isticmaalaa cilmi-baarista dawooyinka ku xayiran.

1.2 Muhiimadda doorashada mowduuca

Sanadihii la soo dhaafay, si loo buuxiyo shuruudaha ilaalinta deegaanka, qalabka polymer hydrogel ayaa si tartiib tartiib ah u soo jiitay dareenka baahsan sababtoo ah sifooyinka wanaagsan ee hydrophilic iyo biocompatibility. Microspheres Hydrogel waxaa laga soo diyaariyey hydroxypropyl methylcellulose sida walxo ceeriin ah tijaabadan. Hydroxypropyl methylcellulose waa ether cellulose ah oo aan ionic ahayn, budo cad, aan ur lahayn oo aan dhadhamin, waxayna leedahay sifooyin aan la bedeli karin oo ah qalabka kale ee polymer synthetic, sidaas darteed waxay leedahay qiimo cilmi baaris sare oo ku yaal beerta polymer.

1.3 Heerka horumarineed ee dal iyo dibadba

Hydrogel waa nooc ka mid ah dawooyinka lagu qiyaaso dawooyinka oo soo jiitay indhaha bulshada caafimaadka adduunka sannadihii u dambeeyay oo si degdeg ah u horumaray. Tan iyo markii Wichterle iyo Lim ay daabaceen shaqadoodii ugu horreysay ee HEMA hydrogels-ku-xiran 1960, cilmi-baarista iyo sahaminta hydrogels ayaa sii waday inay sii qotonto. Bartamihii 1970-meeyadii, Tanaka waxa ay heshay pH-xasaasi ah hydrogels markii la cabbirayay saamiga bararka ee jeelyada acrylamide ee da'da ah, taas oo calaamad u ah tallaabo cusub oo daraasadda hydrogels ah. Dalkaygu wuxuu ku jiraa marxaladda horumarinta hydrogel. Sababtoo ah habka diyaarinta ballaaran ee daawada dhaqameed ee Shiinaha iyo qaybaha adag, way adagtahay in la soo saaro hal badeeco saafi ah marka qaybo badan oo kala duwan ay wada shaqeeyaan, iyo qiyaasta waa weyn, sidaas darteed horumarinta daawada Shiinaha ee hydrogel waxay noqon kartaa mid gaabis ah.

1.4 Qalabka tijaabada ah iyo mabaadi'da

1.4.1 Hydroxypropyl methylcellulose

Hydroxypropyl methyl cellulose (HPMC), oo ka soo jeeda methyl cellulose, waa eter isku dhafan oo muhiim ah, kaas oo iska leh polymers-ka aan biyaha-ionic ahayn, waana ur, aan dhadhamin oo aan sun ahayn.

Warshadaha HPMC waxay ku jirtaa qaab budo cad ama fiber dabacsan oo cad, iyo xalkeeda aqueous waxay leedahay hawlo dusha sare ah, daahfurnaan sare iyo waxqabad xasilloon. Sababtoo ah HPMC waxay leedahay hantida gelation kulaylka, xal aqueous alaabta la kululeeyo si ay u sameeyaan jel ah oo soo da', ka dibna milaan qaboojinta ka dib, iyo heerkulka gelation ee sifooyinka kala duwan ee alaabta waa ka duwan yahay. Sifooyinka kala duwan ee HPMC sidoo kale way kala duwan yihiin. Solubility waxa uu la beddelaa viscosity oo ma saameeyo qiimaha pH. Maqnaanshaha hoose, ayaa sii weynaan kara milanka. Marka maaddada methoxyl ay hoos u dhacdo, dhibicda gel ee HPMC way kordhisaa, milanka biyaha ayaa hoos u dhacaya, dhaqdhaqaaqa dushana wuu yareeyaa. Warshadaha biomedical-ka, waxa inta badan loo isticmaalaa sidii wax-xakamaynta-xakamaynta walxaha polymer-ka ee agabka daahan, agabka filimada, iyo diyaarinta sii-deynta joogtada ah. Waxa kale oo loo isticmaali karaa xasiliye ahaan, wakiilka hakinta, xabagta kiniinka, iyo kor u qaadka viscosity.

1.4.2 Mabda'a

Isticmaalka habka polymerization-ka-joojinta wejiga, iyadoo la adeegsanayo Tween-20, Span-60 kala firdhinta isku-dhafka ah iyo Tween-20 sida kala-firdhinta kala duwan, go'aamiso qiimaha HLB (surfactant waa amphifile leh koox hydrophilic iyo lipophilic group Molecule, qaddarka xajmiga iyo xoogga dheellitirka u dhexeeya kooxda hydrophilic iyo kooxda lipophilic ee molecule surfactant waxaa lagu qeexay qiyaasta qiyaasta qiyaasta hydrophilic-lipophilic ee dusha sare ee Cyclohexane ayaa si fiican u kala firdhi karta xal monomer ah Tijaabada si joogto ah qiyaasta waa 1-5 jeer ka mid ah xalka monomer-ka ee 99% divinyl sulfone oo ah wakiilka iskutallaabta, iyo qadarka wakiilka iskutallaabta ayaa lagu xakameynayaa qiyaastii 10% cufka cellulose-ka qallalan, si ay unugyo toosan oo badan isugu xidhan yihiin oo ay isku xidhmaan qaab-dhismeedka shabakad.

Kicintu aad bay muhiim ugu tahay tijaabadan, xawaaraha guud ahaana waxa lagu xakameeyaa marsha saddexaad ama afraad. Sababtoo ah xajmiga xawaaraha wareega ayaa si toos ah u saameeya xajmiga microspheres. Marka xawaaraha wareeggu uu ka weyn yahay 980r / min, waxaa jiri doona dhacdo darbi ah oo darbi ah, taas oo si weyn u yareyn doonta wax soo saarka alaabta; Wakiilka iskuxiran wuxuu u janjeeraa inuu soo saaro jel-ka-badan, iyo alaabada wareegsan lama heli karo.

2. Qalabka tijaabada iyo hababka

2.1 Qalabka Tijaabada

Dheelitirka elegtarooniga ah, kicinta korantada ee shaqaynaysa, mikroskoob polarizing, cabbirka walxaha Malvern

Si loo diyaariyo microspheres cellulose hydrogel, kiimikooyinka ugu muhiimsan ee loo isticmaalo waa cyclohexane, Tween-20, Span-60, hydroxypropyl methylcellulose, divinyl sulfone, sodium hydroxide, biyo nadiif ah, kuwaas oo dhammaantood Monomers iyo wax lagu daro si toos ah loo isticmaalo daaweyn la'aan.

2.2 Tallaabooyinka diyaarinta ee cellulose hydrogel microspheres

2.2.1 Isticmaalka Tween 20 sida kala firdhisan

Kala dirida hydroxypropylmethylcellulose. Si sax ah u miisaam 2g oo sodium hydroxide ah una diyaari 2% sodiyam hydroxide dareere leh 100ml caag mug leh. Qaado 80ml oo ah xalalka sodium hydroxide ee la diyaariyey oo ku kululee biyaha qubeyska ilaa 50°C, miisaan 0.2g oo cellulose ah oo ku dar xalalka alkaline, ku walaaq ul dhalo ah, ku rid biyo qabow si aad baraf u qabsato, una isticmaal sida marxaladda biyaha ka dib marka la caddeeyo xalka. Isticmaal dhululubo qalin jabisay si aad u cabbirto 120ml of cyclohexane (wajiga saliidda) geli weel saddex luqun leh, ku soo jiid 5ml ee Tween-20 wajiga saliidda siringe, kuna walaaq 700r/min hal saac. Qaado nus ka mid ah wejiga aqueous ee la diyaariyey oo ku dar galaas saddex luqun ah oo walaaq saddex saacadood. Xaddiga divinyl sulfone waa 99%, lagu qasi jiray 1% biyo nadiif ah. Isticmaal pipette si aad 0.5ml oo DVS ah geliso 50ml caag mug leh si aad u diyaariso 1% DVS, 1ml DVS waxay u dhigantaa 0.01g. Isticmaal pipette si aad 1ml ugu qaaddo weelka saddexda luqun. Ku walaaq heerkulka qolka 22 saacadood.

2.2.2 Isticmaalka span60 iyo Tween-20 sida kala firdhis ahaan

Qaybta kale ee wejiga biyaha ee hadda la diyaariyey. Miisaanka 0.01gspan60 kuna dar tuubada tijaabada, kuleyli biyaha qubeyska 65-degree ilaa ay dhalaalayso, ka dibna ku rid dhowr dhibcood oo cyclohexane ah biyaha qubeyska iyada oo la raacayo dhibco caag ah, oo kuleyli ilaa uu xalku noqdo caddaan caano ah. Ku dar galaas saddex luqun ah, ka dibna ku dar 120ml of cyclohexane, ku mayr tuubada tijaabada cyclohexane dhowr jeer, kuleyl 5min, qabooji heerkulka qolka, ku dar 0.5ml ee Tween-20. Ka dib markaad walaaqdo saddex saacadood, 1ml oo DVS ah oo la qasi jiray ayaa lagu daray. Ku walaaq heerkulka qolka 22 saacadood.

2.2.3 Natiijooyinka tijaabada ah

Muunadda la walaaqay waxaa lagu dhex rusheeyay ul dhalo waxaana lagu milmay 50ml oo ethanol ah oo dhammaystiran, cabbirka walxaha waxaa lagu cabbiray cabbirka walxaha Malvern. Isticmaalka Tween-20 sida microemulsion kala firdhisan waa ka dhumuc weyn yahay, iyo cabbirka walxaha la qiyaasay ee 87.1% waa 455.2d.nm, cabbirka qaybta 12.9% waa 5026d.nm. Microemulsion-ka Tween-20 iyo Span-60 kala firdhiso isku dhafan waxay la mid tahay kan caanaha, oo leh 81.7% cabbirka walxaha 5421d.nm iyo 18.3% cabbirka walxaha 180.1d.nm.

3. Ka doodista natiijooyinka tijaabada ah

Emulsifier-ga loogu talagalay diyaarinta microemulsion-ga rogan, inta badan way fiicantahay in la isticmaalo isku dhafka surfactant hydrophilic iyo lipophilic surfactant. Tani waa sababta oo ah milanka hal surfactant ee nidaamka ayaa hooseeya. Ka dib marka labada la isku daro, Kooxaha hydrophilic-ka midba midka kale iyo kooxaha lipophilic ayaa iska kaashada midba midka kale si ay u yeeshaan saameyn milan. Qiimaha HLB sidoo kale waa tilmaame caadi ah oo la isticmaalo marka la dooranayo emulsifiers. Marka la hagaajiyo qiimaha HLB, saamiga laba qaybood oo ka kooban emulsifier waa la wanaajin karaa, waxaana la diyaarin karaa microspheres lebbisan oo badan. Tijaabadan, lipophilic daciif ah Span-60 (HLB=4.7) iyo hydrophilic Tween-20 (HLB=16.7) ayaa loo adeegsaday kala firdhiye ahaan, Span-20 ayaa keligiis loo adeegsaday kala firdhiyey. Natiijooyinka tijaabada ah, waxaa la arki karaa in isku dhafka saameyntu ay ka fiican tahay hal qayb oo kala firdhisan. Microemulsion-ka kala firdhisanaanta xaruntu waa mid lebbisan oo waxay leedahay joogtayn caano u eg; Microemulsion-ka iyadoo la isticmaalayo hal qayb oo kala firdhiso waxay leedahay viscosity aad u sarreeya iyo qaybo cadcad. Meesha ugu sarreysa waxay ka hoos muuqataa kala firdhisanaanta xarunta ee Tween-20 iyo Span-60. Sababta suurtogalka ah ayaa ah in xiisadda isdhexgalka ee nidaamka isku-dhafka ah ee Span-60 iyo Tween-20 ay sarreeyaan, kala-soocida lafteeda ayaa la jejebiyey iyada oo la kariyey xoojinta sare si loo sameeyo Qaybaha ganaaxa ayaa saameyn doona natiijooyinka tijaabada ah. Dhibaatada Tween-20 ee kala firdhisan waa in ay leedahay tiro badan oo silsilado polyoxyethylene ah (n=20 ama wax ka badan), taas oo ka dhigaysa caqabada adag ee u dhexeeya molecules surfactant weyn oo ay adagtahay in ay cufan ku noqoto isdhexgalka. Marka la eego isku dhafka jaantusyada cabbirka walxaha, qaybaha cad ee gudaha ku jira waxay noqon karaan cellulose aan kala firdhin. Sidaa darteed, natiijooyinka tijaabadani waxay soo jeedinayaan in saameynta isticmaalka kala-baxa isku-dhafka ah ay ka fiican tahay, tijaabaduna waxay sii yareyn kartaa qadarka Tween-20 si loo sameeyo microspheres-ka diyaarsan oo dheeraad ah.

Intaa waxaa dheer, qaar ka mid ah khaladaadka habka hawlgalka tijaabada ah waa in la yareeyaa, sida diyaarinta sodium hydroxide ee habka kala dirida ee HPMC, milanka DVS, iwm, waa in la qiyaaso inta ugu badan ee suurtogalka ah si loo yareeyo khaladaadka tijaabada ah. Waxa ugu muhiimsan waa qadarka kala firdhinta, xawaaraha iyo xoojinta kicinta, iyo qadarka wakiilka isku-xidhka ah. Kaliya marka si habboon loo xakameeyo ayaa la diyaarin karaa microspheres hydrogel oo leh kala firdhisan wanaagsan iyo cabbirka walxaha isku midka ah.