Qaadashada cellulose bakteeriyada sida alaabta ceeriin, synthesize 2-hydroxy-3-sulfate propyate cellulose ether. spectrometer-ka infrared ayaa falanqeynaya qaab dhismeedka alaabta. Shuruudaha habka ugu wanaagsan ee isku-dhafka ether-ka bakteeriyada cellulose-ka. Natiijooyinka waxay muujiyeen in awoodda beddelka 2-hydroxy-3-sulfonic acid-ku-saleysan propyate bakteeriyada ether-ka lagu sameeyay xaaladaha hagaajinta ay ahayd 0.481mmol / g.
Erayada Furaha cellulose bakteeriyada; 2-hydroxyl-3-sulfonic acid-ku salaysan gornemine cellulose ether; awoodda isdhaafsiga
Cellulose bakteeriyada synthetic microbial waxay la mid tahay cellulose dhirta ee ka kooban kiimikada iyo qaabdhismeedka kelli. Waa polysaccharide toosan oo ku xidhan gulukoos D-pyrarot lehβ-1, 4-glycoside bonds. Marka la barbardhigo cellulose dhirta, cellulose bakteeriyada waxay leedahay sifo wanaagsan. Waa shabag fiber ultra-micro ah oo ka kooban fiilooyinka ultra-micro. Waxay ku jirtaa qaabka cellulose saafi ah waxayna leedahay hawlo badan oo gaar ah. Dhinacyada qalabka dhawaaqa iyo macdanta saliidda ayaa si weyn loo isticmaalay.
2-hydroxyl-3-sulfonate cellulose ether waa unug muhiim ah oo ka samaysan cellulose kaas oo laga samayn karo qalab sare oo nuugista biyaha ah. Waxa kale oo loo isticmaali karaa nadiifnimo adag oo loogu talagalay adsorption of aions biraha culus iyo borotiinka sida cation. Feng Qingqin, Jie Zhefeng iyo cellulose kale oo loo isticmaalo caws bariis qolof galleyda si ay u diyaariyaan 2-hydroxyl-3-sulfate cellulose ether xoog leh beddelashada cationic acid. Maqaalkani waxa uu isticmaalaa cellulose bakteeriyada sida alaabta ceeriin, synthesizing 2-hydroxyl-3-sulfonic acid-ku salaysan bakteeriyada cellulose ether, iyo isticmaalaa tijaabo orthogonal si ay u bartaan xaaladaha ay synthetic ugu fiican iyo 2-hydroxyl-3-sulfa-sulfa sulfa diyaarisay xaaladdan. Awoodda is-dhaafsiga ee gornemine cellulose ether-ku-salaysan aashitada waxay bixisaa aasaas aragtiyeed oo ku saabsan codsiga dhabta ah ee walxaha.
1. Qaybta tijaabada ah
1.1 Reagents iyo qalabka
Bakteeriyada cellulose (is-sameeyay), sodium hydroxide, sodium carbonate, sodium bisulfite, dioxane, epichlorohydrin, acetone, ethanol, sodium carbonate, reagents kor ku xusan waa darajo gorfaynta.
Incubator/sanduuqa qalajinta (Shanghai-Heng Technology Co., Ltd.); GQF-1 jet Mill (Xarunta budada, Jaamacadda Sayniska iyo Tignoolajiyada ee Nanjing); spectrometer infrared Fourier (Jarmalka); Agilent AAS-3510 spectrophotometer nuugista atomiga.
1.2 Diyaarinta 2-hydroxy-3-sulfopropyl bakteeriyada cellulose eter
1.2.1 Synthesis of cellulose bakteeriyada isku-xiran
Ku dar 10g oo budada cellulose bakteeriyada, 60ml ee epichlorohydrin iyo 125ml oo 2mol ah.·Xalka L-1 NaOH galaas saddex luqun leh oo ku qalabaysan kondenser-ka reflux iyo kiciya, kulaylka dib-u-soo-celinta 1h, filter, iyo iskutallaab ku maydh acetone iyo biyo ilaa hanti dhexdhexaad ah, oo ku qallaji faakuum hoostiisa 60°C si loo helo cellulose bakteeriyada isku-dhafan.
1.2.2 Synthesis of sodium 3-chloro-2 hydroxypropanesulfonate
Miisaanka 104.0gNaHSO3 kuna mil 200mLH2O, oo ha lagu dhufto gaaska SO2. Kuleyl ilaa 70-90°C oo walaaqaya, ka dibna ku dar 160mL epichlorohydrin oo leh funnel daadinta, oo ka jawaab 85°C ilaa 4 saacadood. Alaabta falcelinta waxaa lagu qaboojiyey in ka hooseyso 5°C si aad u miir-geliso alaabta, ka dibna nuug la sifeeyo, la dhaqo, oo la qalajiyo si loo helo badeeco cayriin ah oo jaale ah. Badeecada ceeriin ayaa dib loogu celiyay 1:1 ethanol si loo helo kiristaalo cad.
1.2.3 Isku-darka 2-hydroxy-3-sulfopropyl bakteeriyada cellulose eter
Ku dar 2 g oo ah cellulose bakteeriyada isku-tallaabta ah, xaddi go'an oo ah 3-chloro-2-hydroxypropanesulfonate, 0.7 g oo ah sodium carbonate, iyo 70 ml oo ah dioxane aqueous aqueous galaas saddex-luug ah oo ku qalabaysan kondenser reflux ah iyo kiciya. nitrogen ilaalinta hoosteeda, xakamee heerkul cayiman oo walaaq si aad uga falceliso wakhti go'an, shaandhee, ku maydh acetone iyo biyo una badasho dhexdhexaadnimo, oo faakium-qallaji 60°C si aad u hesho jaale khafiif ah oo adag.
1.3 Falanqaynta qaab-dhismeedka alaabta
Tijaabada FT-IR: kiniinka adag ee KBr, baaxadda tijaabada: 500cm-1～4000cm-1.
1.4 Go'aaminta awoodda sarrifka
Qaado 1-2g oo ah 2-hydroxy-3-sulfopropyl bakteeriyada cellulose ether, ku dar qadar ku habboon oo biyo nadiif ah si aad u qoyso, ka dibna ku shub tiirka sarrifka ee walaaqaya, raaci qadar ku habboon oo biyo nadiif ah, ka dibna isticmaal qiyaastii 100ml 5% Biyo raaci hydrochloric acid, xakamee heerka socodka 3mL daqiiqadii. Dabadeed ku maydh biyo la shiiday ilaa aysidhka ka muujin waayaan marka lagu tijaabiyo methyl orange, ka dibna ku rid ilaa 60mL oo ah sodium chloride oo ay la urursan tahay 1mol L-1, xakamee heerka socodka ilaa 3mL/daqiiqo, oo ku ururi dareeraha Qalabkii Erlenmeyer Ka dibna tiirka ku maydh 50-80mL biyo nadiif ah. Xalka la ururiyey waxaa lagu titrateed 0.1mol·L-1 sodium hydroxide xalka caadiga ah iyadoo la adeegsanayo phenolphthalein sida tilmaame, iyo tirada millilitir ee sodium hydroxide la cunay waxay ahayd VNaOH.
2. Natiijooyinka iyo doodaha
2.1 Tilmaamaha qaabdhismeedka ee cellulose bakteeriyada isku-xiran
Sababo la xiriira soo bandhigida cusub ee C-H, cellulose-ka bakteeriyada ee isku-tallaabta ah waa 2922.98cm-1. Gariirka fidsan ee C-H ee giraanta sonkorta waa la wanaajiyey, iyo meelaha ugu sarreeya ee nuugista ee kooxaha hydroxyl ee 1161.76cm-1 iyo 1061.58cm-1 ee xariiqda muraayadda a ayaa daciifay, kuwaas oo ah kuwa ugu sarreeya nuugista ee kooxaha hydroxyl ee cellulose. Marka la joogo 3433.2cm-1, heerka ugu sarreeya ee nuugista gariirka ee kooxda hydroxyl ee laxiriirta ayaa wali jirta, laakiin xoojinta qaraabada ayaa hoos u dhacda, taas oo muujinaysa in kooxda hydroxyl ee giraanta glucoside aan si buuxda loo bedelin.
2.2 Qaab dhismeedka sodium 3-chloro-2-hydroxypropanesulfonate
3525～3481cm-1 waa gariir fidsan ee ururka hydroxyl O-H bond, 2930.96cm-1 waa gariirka fidinta asymmetric ee C-H, 2852.69cm waa gariir isku fidsan ee C-H, 1227.3cm-1, 1054. 95cm-1 waa gariir fidsan ee S=O, 810.1cm-1 waa gariir fidsan ee COS, iyo 727.4cm-1 waa gariir fidsan ee C-Cl, oo muujinaysa in badeecada la beegsanayo la sameeyay.
2.3 Tilmaamaha qaabdhismeedka ee 2-hydroxy-3-sulfopropyl bakteeriyada cellulose ether
3431cm-1 waa OH fidinta gariirka ugu sarreeya, 2917cm-1 waa heerka ugu sarreeya ee gariirka fidinta CH, 1656cm-1 waa CC fidinta gariirka ugu sarreeya, 1212 ~ 1020cm-1 waa -SO2-antisymmetric iyo gariir fidsan, 658cm-1 gariirka fidinta dammaanadda SO.
2.4 Hagaajinta xaaladaha isku-dhafka ee 2-hydroxy-3-sulfopropyl bakteeriyada cellulose ether
Tijaabada, awoodda sarrifka ayaa loo isticmaalay si loo tijaabiyo tayada 2-hydroxy-3-sulfopropyl ether bakteeriyada cellulose. Qadarka 3-chloro-2 hydroxypropanesulfonate sodium ee lagu daray fal-celinta, xoojinta dioxane xal aqueous, wakhtiga falcelinta iyo heerkulka ayaa sameeyay afar arrimood iyo saddex heer oo tijaabo ah oo orthogonal ah si loo falanqeeyo saameynta arrin kasta oo ku saabsan bakteeriyada cellulose xanthate. . Saamaynta guryaha ester-ka.
Tijaabooyinka orthogonal waxay muujinayaan in isku dhafka ugu fiican ee 4 arrimood ay tahay A2B1C3D. 1 Falanqaynta kala duwan waxay muujinaysaa in heerkulka falcelintu uu saameyn weyn ku leeyahay waxqabadka xayeysiinta ee 2-hydroxy-3-sulfopropyl cellulose ether, iyo qiyaasta 1. 914, oo ay ku xigto xoojinta wakhtiga, dioxane, iyo qadarka quudinta ee 3. -chloro-2 hydroxypropanesulfonate sodium. Awoodda beddelka 2-hydroxy-3-sulfopropyl ether bakteeriyada cellulose ee lagu diyaariyey xaaladaha la hagaajiyey waxay ahayd 0.481mmol/g, taas oo ka sarraysa ta la mid ah geedaha beddelka aashitada ee SE-nooca lagu sheegay buug-gacmeedka.
3. Gabagabo
Iyada oo wax laga beddelayo cellulose bakteeriyada, 2-hydroxy-3-sulfonic acid propyl bakteeriyada cellulose ether ayaa la sameeyay, qaab-dhismeedkeedana waxaa lagu sifeeyay oo la cabbiray awooddeeda sarrifka. Gabagabada soo socota ayaa la soo saaray: 1) 2-hydroxy-3 - Shuruudaha habka ugu wanaagsan ee isku dhafka bakteeriyada cellulose ether sulfopropyl waa: 2g cellulose bakteeriyada iskutallaabta, 3.5g 3-chloro-2-hydroxypropanesulfonate sodium, 0.7g sodium carbonate iyo 7OmI30% dioxane Aqueous solution, falcelinta 70°C ee hoos yimaada ilaalinta nitrogen ee 1h, 2-hydroxy-3-sulfonic acid propyl bakteeriyada cellulose ether ee lagu diyaariyey xaaladdan ayaa leh awood sare oo sarrifka; 2) Kooxda 2-hydroxy-3-sulfonic acid Awoodda beddelka ee ether bakteeriyada propyl waxay ka sarraysaa ta la midka ah SE cellulose resin beddelka aashitada xooggan ee lagu sheegay buug-gacmeedka.
Waqtiga boostada: Mar-06-2023