Na-ewere cellulose nje dị ka akụrụngwa, synthesize 2-hydroxy-3-sulfate propyate cellulose ether. The infrared spectrometer na-enyocha nhazi ngwaahịa. Ọnọdụ usoro kachasị mma maka njikọ nke cellulose ether nke nje bacteria. Nsonaazụ gosiri na ike mgbanwe nke 2-hydroxy-3-sulfonic acid propyate bacterial ether synthesized n'okpuru ọnọdụ njikarịcha bụ 0.481mmol / g.
Okwu: cellulose nke nje bacteria; 2-hydroxyl-3-sulfonic acid dabeere gornemine cellulose ether; ike mgbanwe
Microbial sịntetik bacterial cellulose yiri osisi cellulose na chemical mejupụtara na molekụla Ọdịdị. Ọ bụ polysaccharide kwụ ọtọ jikọtara ya na D-pyrarot glucoseβ-1, 4-glycoside bond. E jiri ya tụnyere cellulose osisi, cellulose nje nwere àgwà ka mma. Ọ bụ eriri ultra-micro eriri nke nwere eriri ultra-micro mejupụtara. Ọ dị n'ụdị cellulose dị ọcha ma nwee ọtụtụ ọrụ pụrụ iche. A na-eji akụkụ nke akụrụngwa egwu na egwu mmanụ eme ihe n'ọtụtụ ebe.
2-hydroxyl-3-sulfonate cellular cellulose ether bụ ihe dị mkpa cellulose mmepụta nke nwere ike mere nke elu mmiri absorption ihe. Enwere ike iji ya dị ka ịdị ọcha siri ike maka mgbasa ozi nke ion metal dị arọ na protein dị ka cation. Feng Qingqin, Jie Zhefeng na cellulose ndị ọzọ eji na osikapa shei ọka ahịhịa iji kwadebe 2-hydroxyl-3-sulfate cellulose ether ike acid cationic mgbanwe. Edemede a na-eji cellulose nje mee ihe dị ka akụrụngwa, na-emepụta 2-hydroxyl-3-sulfonic acid nke dabeere na nje cellulose ether, ma na-eji nyocha orthogonal na-amụ ọnọdụ ya kacha mma na 2-hydroxyl-3-sulfa-sulfa sulfa kwadebere n'okpuru ọnọdụ a. Ike mgbanwe nke acid gornemine cellulose ether nke dabeere na acid na-enye ndabere usoro maka itinye ngwa nke ihe ahụ n'ezie.
1. Akụkụ nnwale
1.1 Reagents na ngwa
Bacterial cellulose (onwe-mere), sodium hydroxide, sodium carbonate, sodium bisulfite, dioxane, epichlorohydrin, acetone, ethanol, sodium carbonate, reagents n'elu bụ nke nyocha ọkwa.
Igbe incubator / ihicha (Shanghai-Heng Technology Co., Ltd.); GQF-1 igwe igwe jet (Ebe ntụ ntụ, Mahadum Sayensị na Nkà na ụzụ Nanjing); spectrometer infrared anọ (Germany); Agilent AAS-3510 atomiki absorption spectrophotometer.
1.2 Nkwadebe nke 2-hydroxy-3-sulfopropyl nje cellulose ether
1.2.1 Nkwekọrịta nke cellulose nje bacteria na-ejikọta ya
Tinye 10g nke cellulose ntụ ntụ, 60ml nke epichlorohydrin na 125ml nke 2mol.·Ngwọta L-1 NaOH n'ime flask olu atọ nke nwere reflux condenser na onye na-akpali akpali, na-ekpo ọkụ maka reflux maka 1h, nzacha, na cross-saa na acetone na mmiri na-ajụ ihe onwunwe, na akpọnwụwo n'okpuru agụụ na 60.°C iji nweta cellulose nje bacteria na-ejikọta ya.
1.2.2 Synthesis nke sodium 3-chloro-2 hydroxypropanesulfonate
Tulee 104.0gNaHSO3 wee gbarie ya na 200mLH2O, ka ejupụta ya na gas SO2. Kpoo ọkụ ruo 70-90°C na-akpali akpali, wee tinye 160mL epichlorohydrin na nsị nsị, wee meghachi omume na 85.°C maka 4h. Emere ngwaahịa mmeghachi omume ahụ ka ọ dị n'okpuru 5°C iji mee ka ngwaahịa ahụ kristal, wee kpochaa, sachaa, ma akpọnwụọ iji nweta ngwaahịa crude edo edo. Ejiri 1: 1 ethanol megharịa ngwaahịa crude ahụ iji nweta kristal ọcha.
1.2.3 Nkwekọrịta nke 2-hydroxy-3-sulfopropyl nje cellulose ether
Tinye 2 g nke cellulose nje na-ejikọta ya, ụfọdụ ego nke 3-chloro-2-hydroxypropanesulfonate, 0.7 g sodium carbonate, na 70 ml nke dioxane mmiri mmiri n'ime flask atọ nwere olu nke nwere reflux condenser na onye na-akpali akpali. nitrogen N'okpuru nchebe, jikwaa ụfọdụ okpomọkụ ma kpalie imeghachi omume ruo oge ụfọdụ, nzacha, sachaa na acetone na mmiri na-atụgharị gaa na nnọpụiche, wee kpochaa na 60.°C iji nweta ìhè edo edo siri ike.
1.3 Ngwaahịa nhazi nyocha
Nnwale FT-IR: mbadamba nkume KBr siri ike, oke ule: 500cm-1～4000 cm - 1.
1.4 Mkpebi nke ike mgbanwe
Were 1-2g nke 2-hydroxy-3-sulfopropyl bacterial cellulose ether, tinye kwesịrị ekwesị ego nke distilled mmiri ka sook, wee wụsa ya n'ime mgbanwe kọlụm na-akpali akpali, itucha na kwesịrị ekwesị ego nke distilled mmiri, wee na-eji banyere 100mL 5% Hydrochloric acid sachaa, jikwaa ọsọ ọsọ nke 3ml kwa nkeji. Mgbe ahụ, were mmiri dị nro sachaa ruo mgbe ọ naghị egosi acidity mgbe a na-anwale ya site na orange methyl, wee jiri ihe dị ka 60mL nke sodium chloride na ntinye nke 1mol L-1, na-achịkwa ọnụego na-erute n'ihe dịka 3mL / min, wee kpokọta ihe na-esi na ya. Erlenmeyer flask. Mgbe ahụ, sachaa kọlụm na 50-80mL mmiri distilled. A na-etinye ihe ngwọta a chịkọtara na 0.1mol·Ngwọta ọkọlọtọ L-1 sodium hydroxide na-eji phenolphthalein dị ka ihe ngosi, yana ọnụọgụ milliliters nke sodium hydroxide riri bụ VNaOH.
2. Nsonaazụ na mkparịta ụka
2.1 Nhazi nhazi nke cellulose nje bacteria na-ejikọta ya
N'ihi iwebata nke ọhụrụ C-H, cellulose nje bacteria na-ejikọta cross bụ 2922.98cm-1. Mgbatị na-agbatị nke C-H na mgbanaka shuga na-abawanye, na njirimara mmịpụta nke otu hydroxyl dị na 1161.76cm-1 na 1061.58cm-1 nke ahịrị spectral a na-ebelata, nke bụ njirimara nnabata nke otu hydroxyl na cellulose. Na 3433.2cm-1, mgbanaka mgbakasị ahụ nke otu hydroxyl jikọtara ya ka dị, mana ike ikwu ya na-ebelata, na-egosi na agbanwebeghị otu hydroxyl na mgbanaka glucoside.
2.2 Nhazi nhazi nke sodium 3-chloro-2-hydroxypropanesulfonate.
3525～3481cm-1 bụ ịma jijiji na-agbatị nke njikọ hydroxyl O-H bond, 2930.96cm-1 bụ asymmetric na-agbatị ịma jijiji nke C-H, 2852.69cm bụ ịma jijiji na-agbatị symmetrical nke C-H, 1227.3cm-1, 1054. 95cm-1 bụ ihe na-agbatị vibration nke S = O, 810.1cm-1 bụ ndị na-agbatị vibration nke COS, na 727.4cm-1 bụ na-agbatị vibration nke C.-Cl, na-egosi na e mepụtara ngwaahịa ezubere iche.
2.3 Nhazi nhazi nke 2-hydroxy-3-sulfopropyl nje cellulose ether
3431cm-1 bụ OH na-agbatị elu ịma jijiji, 2917cm-1 bụ juputara CH na-agbatị vibration elu, 1656cm-1 bụ CC na-agbatị vibration elu, 1212 ~ 1020cm-1 bụ -SO2-antisymmetric na symmetric na-agbatị vibration, 658cm-1 bụ. na SO bond na-agbatị vibration.
2.4 nkwalite nke ọnọdụ njikọ maka 2-hydroxy-3-sulfopropyl nje cellulose ether
Na nnwale ahụ, a na-eji ikike mgbanwe iji nwalee àgwà nke 2-hydroxy-3-sulfopropyl nje cellulose ether. Ọnụ ego nke 3-chloro-2 hydroxypropanesulfonate sodium agbakwunyere na mmeghachi omume, ntinye nke ngwọta dioxane aqueous, oge mmeghachi omume na okpomọkụ emeela ihe anọ na ọkwa atọ nke nyocha orthogonal iji nyochaa mmetụta nke ihe ọ bụla na cellulose xanthate nje bacteria. . Mmetụta nke akụrụngwa ester.
Nnwale orthogonal na-egosi na ngwakọta kacha mma nke ihe anọ bụ A2B1C3D. 1 Nyocha nyocha na-egosi na okpomọkụ mmeghachi omume nwere mmetụta kachasị ukwuu na arụmọrụ adsorption nke 2-hydroxy-3-sulfopropyl cellulose ether, na nso nso bụ 1. 914, na-esote ntinye oge, dioxane, na nri nri nke 3. chloro-2 hydroxypropanesulfonate sodium. Ike mgbanwe nke 2-hydroxy-3-sulfopropyl nje cellulose ether nke a kwadebere n'okpuru ọnọdụ kachasị mma bụ 0.481mmol/g, nke dị elu karịa nke ụdị SE-ụdị cellulose siri ike acid cation mgbanwe osisi ndị a kọrọ na akwụkwọ ntuziaka.
3. Nkwubi okwu
Site n'ịgbanwe cellulose nke nje bacteria, 2-hydroxy-3-sulfonic acid propyl bacterial cellulose ether na-emepụta, na e ji mara usoro ya ma tụọ ike mgbanwe ya. Edepụtara nkwubi okwu ndị a: 1) 2-hydroxy-3 - Ọnọdụ usoro kachasị mma maka njikọ nke sulfopropyl bacterial cellulose ether bụ: 2g cross-linked bacterial cellulose, 3.5g 3-chloro-2-hydroxypropanesulfonate sodium, 0.7g sodium carbonate. na 7OmI30% dioxane Aqueous ngwọta, mmeghachi omume na 70°C n'okpuru nchebe nitrogen maka 1h, 2-hydroxy-3-sulfonic acid propyl bacterial cellulose ether kwadebere n'okpuru ọnọdụ a nwere ikike mgbanwe dị elu; 2) 2-hydroxy-3-sulfonic acid otu Ike mgbanwe nke propyl bacterial cellulose ether dị elu karịa nke ụdị SE ụdị cellulose siri ike acid mgbanwe resin nke a kọrọ na akwụkwọ ntuziaka.
Oge nzipu: Mar-06-2023